Tanımlayıcı Ġstatistikler

• Sabe-se pelos estudos bibliográficos que a linhagem de Pseudomonas aeruginosa mantém-se ativa em uma faixa muito ampla de pH (4 a 10) e que o mesmo influência significativamente na produção de biossurfactante (HOROWITS et al., 1991, MAKKAR & CAMEOTRA, 1997, PERSSON et al. 1988, DEL'ARCOS, 1999, FERREIRA, 1999). Em virtude disso, propõe-se realizar ensaios no reator, variando o pH em 5,0, 6,0 e 8,0 mantendo-se constante as demais variáveis (temperatura, agitação e aeração). Este estudo poderá ser realizado nas condições operacionais otimizadas.

• Neste estudo foi determinada a importância da adição de nitrogênio no meio de produção (2° planejamento), para síntese de biossurfactante por Pseudomonas aeruginosa PALC. Assim, propõe-se testar outras duas fontes de nitrogênio, sendo elas: NaNO3 e (NH4)2SO4 encontradas na literatura (MERCADÉ et al., 1992, MAKKAR et

al., 1996, TURKOVSKAYA et al., 1999, KIM et al., 1999, ABALOS et al., 2000, SANTA ANNA et al., 2001, SANTOS et al., 2002). Poderá ser mantidas as condições operacionais selecionadas anteriormente, e estas fontes serão testadas na mesma concentração de nitrogênio otimizado.

• Caracterizar a estrutura molecular do raminolipídeo e determinar sua quantidade produzida;

• Determinar a cinética de consumo de substrato durante as 48 horas de processo fermentativo;

• Aplicar o biossurfactante produzido em planta de recuperação de petróleo ou em processo de biorremediação.

REFERÊNCIAS BIBLIOGRÁFICAS

ABALOS, A., PINAZO, A., INFANTE, M. R. CASALS, M., GARCIA, F., MANRESA, A. Physicochemical and antimicrobial properties of new rhamnolipid produced by Pseudomonas aeruginosa AT10 from oil refinery wastes. Langmuir, v. 17, p. 1367-1371, 2001.

ANDRÉS, C.; MERCADÉ, E.; GUINEA, J.; MANRESA, A. 7,10-Dihydroxy-8 (E) – octadecanoic acid produced by Pseudomonas 42 A2: evaluation of different cultural parameters of the fermentation. World Journal of Microbiology and Biotechnology, v. 10: p.106-109, 1994.

ANGGRAINI-SÜß: Reprocessing of used vegetable oil as fuel for diesel engines or heaters. Artigo apresentado no I Congresso Brasileiro-Alemão para Energias Renováveis, Fortaleza, setembro de 1999.

ARIMA, K., KAKINUMA, A., TAMURA, G. Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of Fibrin clot formation. Biochemical and Biophysical Research Communications, v.31, n.3, 1968.

ASSOCIAÇÃO BRASILEIRA DAS INDÚSTRIAS DE ÓLEOS VEGETAIS (ABIOVE). Complexo soja no Brasil em 2007, São Paulo. Disponível em (http://www.abiove.com.br). Acesso em: 14 abril 2007.

BABU, K.S.; AJITH-KUMAN, P.V.; KUNHI, A.A.M. Simultaneous degradation of 3- chlorobenzoate and phenolic compounds by a defined mixed culture of Pseudomonas aeruginosa ssp. World Journal of Microbial & Biotechnology, v. 11, p. 148-152, 1995.

BAILEY, J. E., & OLLIS, D. F.. Biochemical Engineering Fundamentals, second edition, McGraw-Hill, Singapore, pp. 404, (1986).

BANAT, I. The isolation of a thermophilic biosurfactant producing Bacillus sp. Biotechnology Letters, v.15, n.6, p.591-594, 1993.

BANAT, I.M., SAMARAH, N., MURAD, M., ROENE, R., BANERJU, S. Biosurfactant production and use in oil tank clean-up. World Journal of Microbiology and Biotechnology, v.7, p.80-88,1991.

BANAT, I. M. Biosurfactant production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a review. Bioresearch Technology, 51: 1- 12, 1995.

BANAT, I. M.; Biosurfactant. v. 41, p. 198, 2000.

BANAT, I. M., RHAMAN, K. S. M., RHAMAN, T. J., MARCHANT, R., MCCLEAN, S. Rhamnolipid bi8osurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials. Biotechnology Prog. v. 18, p. 1277-1281, 2002. BATISTA, E., MONNERAT, S., KATO, K., Liquid–Liquid Equilibrium for Systems of

Canola Oil, Oleic Acid, and Short – Chain Alcohols, J. Chem. Eng. Data, v.44, n.6, p.1360 – 1364, 1999.

BENINCASA, M., CONTIERO, J., MANRESA, M. A., MORAES, I. O. Rhamnolipid production by Pseudomonas aeruginosa LBI growing on soapstock as the sole carbon source. Journal of Food Engineering 54 p. 283-288, 2002.

BERNHEIMER, A.W., AVIGAD, L.S. Nature and properties of cytological agent produced by Bacillus subtilis. Journal of General Microbiology, v. 61, p.361-369, 1970.

BERTRAND, J.C., BONIN, P., GOUTX, M., GAUTHIER, M., MILLE, G. The potential application of biosurfactants in combatting hydrocarbon pollution in marine environments. Research in Microbiology, v.145, n.1, 1994.

BILLEK.G. Heated fats in the diet. In: PADLEY, F. B.; PODMORE. J (Eds). The role of fats in human nutrition. Chichester: Ellis Horwood, cap. 12, p. 163-172, 1985. BIODIESEL BRASIL. Em casa e nas escolas. Departamento. de Química - USP/RP.

Ribeirão Preto-SP n. 274, 2007. Disponível em www.biodieselbrasil.com.br. Acesso em 25 julho.

BOGNOLO, G. Biosurfactants as emulsifying agents of hydrocarbons. Colloids and surfaces A: Physicochemical and Engineering Aspects. 152: 41 - 52, 1999.

BODOUR, A. A., DREES, K. P., MAIER, M. R. Distribution of Biosurfactant- Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils. Applied and Environmental Microbiology, v. 69, n. 6, p. 3280–3287, 2003.

BOULTON, C. A., RATLEDGE, C. Biosynthesis of lipid percursors to surfactant production. Biosurfactants and Biotechnology, New York (USA): Ed. KOSARIC, N.; CAIRNS, W. L. & GRAY, N. C. C.; DEKKER, M. Inc., p. 47 - 87, 1987. BOX, G. E. P. HUNTER, W. G., HUNTER, T.S. Statistics for Experimenters. United

States of America and Canada, John Wiley & Sons, Inc., 1978.

BRADY, J.E., HUMISTON,G.E. Química Geral. Rio de Janeiro: Livros Técnicos e Científicos, 1a ed, 1981.

CABALLERO-CÓRDOBA, G.M.; PACHECO, M.T.B.; SGARBIERI, V.C. Composição química de biomassa de levedura integral (Saccharomyces cereveisiae) e determinação do valor nutritivo da proteína, em células íntegras ou rompidas mecanicamente. Ciência e Tecnologia de Alimentos, v. 17, n. 2, p. 102- 106, 1997.

CABRAL FILHO, S.L.S. Avaliação do resíduo de cervejaria em dietas de ruminantes através de técnicas nucleares e correlatadas. Piracicaba, 1999.

CAMEOTRA, S.S.; MAKKAR, R.S. Synthesis of biosurfactants in extreme conditions. Applied microbiology and Biotechnology, v. 50, p. 520-529, 1998.

CAMERON, D. R., COOPER, D. G., NEUFELD, R. J. The mannoprotein of Saccharomyces cerevisiae is an effective biomulsifier. Applied and Environmental Microbiology, Vol. 54, n. 6, p. 1420-1525, 1988.

CIRIGLIANO, M. C., CARMAN, G. M., Isolation of a Bioemulsifier from Torulopsis lipolytica. Applied end Environmental Microbiology, Vol. 48, n. 4, p. 747-750, 1984.

COOPER, D.G., MACDONALD, C.R., DUFF, S.J., KOSARIC, N. Enhanced production of surfactin from Bacillus subtilis by continuous product removal and metal cation additions. Applied and Environmental Microbiology, v.42, n.3, p.408-412, 1981.

COOPER, D.G., PADDOCK, D.A. Torulopsis petrophilum and surface activity. Applied and Environmental Microbiology, v.46, n.8, p.1426-1429, 1983.

COOPER, D.G., PADDOCK, D.A. Production of a biosurfactant from Torulopsis bombicola. Applied and Environmental, v.47, n.1, p.173-176, 1984.

COOPER, D.G., ZAJIC, J.E. Surface-active compounds from microorganism. Advances in Applied Microbiology, v.26, p.229 - 252, 1980.

CHRISTOFI, N., IVSHIRA, I. B. Microbial and their use in field studies of soil remediation. Journal Applied Microbiology. V. 93, p. 915-929, 2002.

DAVILA, A., MARCHAL, F., VANDECASTEELE, J. Kinetics and balance of fermentation free product inhibition sophorose lipid production by Torulopsis bambicola. Apllied and Microbiology Biotechnology, n. 38, p. 6-11, 1992.

DE LUNA, M. J., RUFINO, D. R., DE GUSMÃO, A. C., SARUBBO, A. L., TAKAKI, M. G. Produção de biosurfactante por Candida glabrata utilizando óleo de algodão como substrato. SINAFERM XV Simpósio Nacional de Bioprocessos - Recife, 2005.

DE ROUBIN, MR, MULLIGAN, C. N., GIBBS, F. G., Correlation of enhanced surfactin production with decreased isocitrate dehydrogenase activity. Canadian Journal of Microbiology, Vol. 35, p. 854-859, 1989.

Desai, J.D. Microbial surfactants: evaluation, types, production and future applications. Journal of Science and Industrial Research, v.46, p.440-449, 1987.

Desai, J.D., Banat, I.M. Microbial production of surfactants and their commercial potential. Microbiology and Molecular Biology Reviews, v.61, n.1, p.47-64, 1997. DESHPANDE M., DANIELS, L. Evaluation of sophorolipid biosurfactant production

by Candida bombicola using animal. Bioresource Technology. Vol. 54, p. 143- 150, 1995.

DEZIEL, E.; LÉPINE, F.; DEMRIE, D.; BOISMEN, D.; MAMER, O.A.; VILLEMUR, R. Liquid chromatography/mass spectrometry analysis of mixtures of rhamnolipids produced by Pseudomonas aeruginosa strain 57RP grown on mannitol or naphthalene. Biochemical et Biophysical Acta, v. 1440, p. 244-252, 1999.

DÉZIEL, E.; PAQUETE, G.; VILLEMUR, R.; LÉPINE, F. BISAILLON, J.G. Biosurfactant production by a soil Pseudomonas strain growing on polycyclic aromatic hydrocarbons. Applied and Environmental Microbiology, v. 62, n. 6, p. 1908-1912, 1996.

DOBARGANES, M.C., MÁRQUEZ-RUIZ, G. Regulation of used frying fats and validity of quick tests for discarding the fats. Grasas Aceites, Sevilla, v.49, n.3/4, p.331-334, 1998.

DUBOIS, M.; GILLES, K.A.; HAMILTON, J.K.; REBERS, P.A.; SMITH, F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, v. 28; n. 3, p. 350-356, 1956.

DUVNJAK, Z., COOPER, D.G., KOSARIC, N. Production of surfactant by Arthrobacter paraffineus ATCC19558. Biotechnology and Bioengineering, v.24, p.165-175, 1983.

DUVNJAK, Z., KOSARIC, N. Production and release of surfactant by Corynebacterium lepus in hydrocarbon and glucose media. Biotechnology Letters, v.7, n.11, p.793-796, 1985.

ESPUNY, M. J., EGIDO, S., MANRESA, I. R. A., MERCADÉ, M. E. Nutritional requerements of a biosurfactant producing strain Rhodococcus sp. 51t7. Biotechnology Letters, Vol. 18, n. 5 p. 521-526, 1996.

FARIA, A. A.; LELES, M. I. G.; IONASHIRO, M., et al. Estudo da Estabilidade Térmica de Óleos e Gorduras Vegetais por TG/DTG e DTA, Ecl. Quím, São Paulo, v. 27, p. 111-119, 2002.

FLASZ, A. ROCHA, C. A., MOSQUERA B., SAJO, C. A comparative study of the toxicity of a synthetic surfactant and one produced by Pseudomonas aeruginosa ATCC 55925, Medical Science Research. v. 26, p. 181–185, 1998.

FERNANDES, F. S., REIS-COSTA, L., KRONKA A. M., COPPEDE, J. S., BENINCASA, M. Aproveitamento de resíduos gerados no refino de óleos vegetais para a produção de biossurfactantes. SINAFERM XV Simpósio Nacional de Bioprocessos - Recife, 2005.

FIECHTER, D. Biosurfactants: moving towards industrial application. Trends in Biotechnology, v.10, p. 208-216, 1992.

FOX, S. L., BREHM, M. A., ROBERTSON, E. P., JACKSON, J. D., THOMAS, C. P., BALA, G. A. Comparitive analysis of microbially madiated oil recovery by surfactants produced by Bacillus licheniformis and Bacillus subtilis. In: Premuzic, E. T., Woodhead, A. (Eds.), Developments in Petroleum Science. Elsivier, New York, p. 143 - 150, 1993.

FRANCY, D.S., THOMAS, J.M., RAYMOND, R.L., WARD, C.H. Emulsification of hydrocarbons by subsurface bacteria. Journal of Industry Microbiology, n.8, p.237-246, 1991.

FURCO, A. M. Produção de biomassa de levedura em destilarias de álcool. In: Anais do workshop sobre produção de biomassa de levedura: utilização em alimentação humana e animal, Instituto de Tecnologia de Alimentos, Campinas, SP, p.52-58, 1996.

GEORGIOU, G., LIN, SC, SHARMA, M.M. Surface-active compounds from microorganisms. Biotechnology, v. 10, p. 60-65, 1992.

GERSON, D. F. Biosurfactantes: production - properties - applications. Editado por Naim Kosaric. 269 - 286, 1993.

GIESE, J. Fats, Oils, and Fat Replaces. Food Technology - Especial Repot, 1996.

GOBBERT, U., LANG, S., WAGNER, F. Sophorose lipid formation by resting cells of Torulopsis bombicola. Biotechnology Letters, n. 6, p. 225-230, 1984.

GONÇALVES, C.; BATISTA, E.; MEIRELLES, A.J.A. Liquid–Liquid Equilibrium Data for the System Corn Oil + Oleic Acid + Ethanol + Water at 298.15 K, J. Chem. Eng. Data, v.47, n.3, p.416 – 420, 2002.

GUERRA-SANTOS, L., KAPPELI, O., FIECHTER, A. Pseudomonas aeruginosa biosurfactant production in continuos culture with glucose as carbon source. Apllied and Environmental Microbiology, Vol. 48, n. 2, p. 301-305, 1984.

GREAVES, K. A., WILSON, M. D., RUDEL, L. L., WILLIAMS, J. K., WAGNER, J. D. Consumption of Soy Protein Reduces Cholesterol Absorption Compared to Casein Protein Alone or Supplemented with an Isoflavone Extract or Conjugated

Equine Estrogen in Ovariectomized Cynomolgus Monkeys. J. Nutr. 130: 820-826, (2000).

HABA, E., ESPUNY, M. J., BUSQUETS, M., MANRESA, A. Screening and production if rhamnolipidis by Pseudomonas aeruginosas 47T2 NCIB 40044 from waste frying oil. J. Appl. Microbiol. Vol. 88 p. 379-387, 2000.

HALÁSZ, A.; LÁSZTITY, R. Use of yeast biomass in food production. CRC Press, Boca Raton, 1991.

HAUSER G., and M. L. KARNOVSKY, Fed. Proc. Vol 16, p. 193- 210, 1957. HELENIUS, A. E SIMONS, K., Solubilization of membranes by detergents. Biochimistry. Biophys. Acta, 415, 29-79, 1975.

HELENIUS, A., SIMONS, K., Solubilization of membranes by detergents. Biochemica et Biophysica Acta. Amsterdam, v.415. p.29-79, 1975.

HIDALGO, F.J.; ALAIZ, M.; ZAMORA, R. Determination of Peptides and Proteins in Fats and Oils, Anal. Chem., v.73, n.3, p.698–702, feb. 2001.

HINZEL, W. L.; Solution Chemistry of Surfactants, Mittal, K. L., ed., Plenunn Press: New York, 1979.

HIRAYAMA, T., KATO, I. Novel rhamnolipids from Pseudomonas aeruginosa. FEBS Letter, n. 139, p. 81-85, 1982.

HOMMEL, R.K. Formation and physiological role of biosurfactants produced by hydrocarbon utilizing microorganisms. Biodegradation, v.1, p.107-119, 1990. HOROWITZ, S., GILBERT, I. N., GRIFFIN, W. M., Isolation and characterization of a

surfactant produced by Baccilus licheniformis. Journal of Industrial Microbiology, Vol. 6, p. 243-248, 1990.

IRVINE, T.S. Laboratory fermenters In: Fermentation: apractical approach, Ed. B. McNeil &L.M. Harvey. Oxford University Press, p 17-38, 1990.

ITOH, S., INOUE, S. Sophorolipds from Torulopsis bombicola: possible relation to alkane uptake. Applied and Environmental Microbiology, n. 43, p. 1278-1283, 1982.

ITOH, S. and T. SUZUKI. Effect of rhamonolipids on growth of Pseudomonas aeruginosa mutant deficient in n-paraffin-utilizing ability. Agric. Biol. Chem. 36: 2233 - 2235, 1971.

JAIN, D. K. THOMPSON, D. L. C., LEE, H., TREVORS, J. T. A drop-collapsing test for screening surfactant producing microorganisms. Journal of Microbiology Methods, n. 13, p. 271-279, 1992.

JAVAHERI, M., JENNEMAN, G. E., MCINERNEY, M. J., KNAPP, R. M., Anaerobic production of a biosurfactant by Baccilus licheniformis JF-2. Applied and Environmental Microbiology, Vol. 50, n.3, p. 698-700, 1985.

KANTIN, R. Poluição aquática por detergentes: aspectos gerais. Rio de Janeiro: Engenharia Sanitária, v. 19, n. 1, p. 82-85, 1980.

KAPPELI, O., MÜLLER, M., FIECHTER, A. Chemical and structural alterations at the cell surface of Torulopsi tropicalis induced by hydrocarbon substrate. Journal of Bacteriology, v. 133, p. 952-958, 1986.

KAPPLAN, N., ROSENBERG, E. Exopolysaccharide distribution of and biomulsifier production by Acinetobacter calcoaceticus BD4 and BD413. Apllied and Environmental. Vol. 44, n. 6, p. 1335-1341, 1982.

KARANTH, N. G. K.; DEO, P. G., VEENANADING. Microbial production of biosurfactants and their importance. Current Science, v. 77, p. 116 - 126, 1999. KIM, S. H., LIM, E. J., LEE, J. D., LEE, T. H. Purification and charaterization of

biosurfactants from Nocardia sp. L-417. Biotechnology Applied Biochemistry. Vol. 31, p. 249-253, 2000.

KINSELLA, J.E.; SHETTY, H.J. Yeast protein recovery, nutritional and functional properties. Advances in Experimental Medicine and Biology, v. 107, n. 5, p. 97- 825, 1978.

KITAMOTO, D., YANAGISHITA, H., SHINBO, T., NAKANE, T., KAMAISAWA, C., NAKAHARA, T. Surface active properties and antimicrobial activities of mannosylerythritol lipids as biosurfactants produced by Candida antartica. Journal of Biotechnology, n. 29, p. 91-96, 1993.

KIYOHARA, H; NAGAO, K; YANA, K. Rapid screen for bacteria degrading water insoluble solid hydrocarbons on agar plates. Applied and Environmental Microbiology, v.13, p.257-266, 1982.

KOCH, A.K.; KAPPELI, O.; FIECHTER, A.; REISER, J. Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. Journal of Bacteriology, v. 173, n. 13, p. 4212-4219, 1991.

KOSARIC, N., GRAY, N.C.C., CAIRNS, W.L. Microbial emulsifiers and de- emulsifiers. In: Rehm, H. J., Reed, G. (eds). Biotechnology, v. 3, cap. 3i, p. 576- 591, 1983.

KOSARIC, N., GRAY, N.C.C., CAIRNS, W.L., STECHEY, D., WOOD, J. The role of notrogen in multiorganism strategies for biosurfactan production. JAOCS, Vol 61, n. 11, 1987.

LANG, S.; WAGNER, F. Structure and properties of biosurfactants. In:Biosurfactants and Biotecnology, ed. N. Kosaric, W.L. Cairns & N.C.C. Gray. Marcel Dekker, New York, NY, pp 1-21, 1987.

LANG, S.; WULLBRANDT, D. Rhamnose lipid – Biosynthesis, Microbial Production and Application Potenctial. Appl. Microbiol. Biotechnol. v. 51, p. 22-32, 1999. LEE, K., KIM, J. H. Distribuition of substrates carbon in sophorose lipid production by

Torulopsis bombicola. Biotechnology Letters, Vol. 15, n. 3, p. 263-266, 1993. LEGISLAÇÃO, Resolução CNNPA nº. 20/77, DOU de 26/07/1977. Disponível em

http://e-legis.anvisa.gov.br/leisref/public. Acesso em 21 janeiro 2007.

LICHTENBERG, D., ROBSON, R.J. E DENNIS, E.A., Solubilization of phospholipids by detergents. Structural and kinetic aspects. Biochim. Biophys. Acta, 737, 285- 304, 1983.

LIN, S.C. Biosurfactants: recent advances. Journal of Chemical Technology and Biotechnology, v.66, p. 109-120, 1996.

LIN, SC, CARSWELL, K. S., SHARMA, M. M., GEORGIOU, G. Continuous production of the lipopeptide biosurfactant of Bacillus licheniformis LF-2. Applied Microbiology and Biotechnology, Vol. 41, p. 281-285, 1994.

Microbially produced rhamnolipid as a source of rhamnose. Biotechnology and Bioengineering, v. 33, p. 365-368, 1989.

LIZ, Y. LANG, S. WAGNER F. WITTE L., and V. WRAY, Appl. Environ. Microbiol. Vol. 48, p. 610, 1984.

LUCAS, E. F. Tensoativos. Seminário de Mestrado Mmp-751. Rio de Janeiro: Instituto de Macromoléculas, UFRJ, 1988.

LUEDEKING, R., & PIRET, E. L. A kinetic study of the lactic acid fermentation: batch process at controlled pH. J. Biochemistry Microbiology Technology Engineering, 1, 393-412, (1959).

MADIGAN, M.T.; MARTINKO, J.M.; PARKER, J. Brock Biology of Microorganisms, 10th edition, Prentice Hall/Pearson Higher Education Group, Upper Saddle River, NJ. ISBN 0-13-066271-2, 2003.

MAKKAR, R. S., CAMEOTRA, S.S. Biosurfactant production by a thermophilic Bacillus subtilis strain. Journal of Industrial Biotechnology, v.18, p.37-42, 1997. MAKULA, R. A., FINNERTY, W. R., Microbial assimilaton of hydrocarbons: cellular

distribuition of fatty acids. Journal of Bacteriology. Vol. 112, n. 1, p. 398-407, 1972.

MARGARITIS, A., KENNEDY, K., JASIC, J. E., GERSON, D. F. Biosurfactant production by Nocardia erythropolis. Development of Industrial Microbiology. N. 20, p. 623-630, 1979.

MARQUARDT, D. W. An algorithm for least-squares estimation of nonlinear parameters. Journal of the Society for Industrial and Applied, 11, 431-441, 1963. MATA-SANDOVAL, J. C., TORRENTS, A. High-performance liquid chromatography

method for the characterization of rhamnolipid mixtures produced by pseudomonas aeruginosa UG2 on corn oil. J. Chromatography, v. 864, n. 2, p. 211-20, 1999.

MATSUFUJI, M.; NAKATA, K.; YOSHIMOTO, A. High production of rhamnolipids by Pseudomonas aeruginosa growing on ethanol. Biotechnology Letters, v.19, n.12, p.1213-1215, 1997.

MAYER, R.M.; SOBERÓN-CHAVEZ, G. Pseudomonas aeruginosa rhamnolipids: biosynthesis and potential applications. Applied Microbiology and Biotechnology, v. 54; p 625-633, 2000.

MERCADE, M. E. Prodducción de biotensioactivos a partir de las aguas residuales de as amazaras, PhD thesis, Universuty of Barcelona, Spain, 1990.

MERCADE, M. E. and MANRESA, M. A. The use of agroindustrial by products for biosurfactant production. Journal American Oil Chemical Society, v. 71, p. 61 - 64, 1994.

MERCADÉ, M. E.; MANRESA, M. A.; ROBERT, M.; ESPUNY, M. J.; DEANDRES, C. and GUINEA, J. Olive oil mill effluent (Oome) - mew substrate for biosurfactant production. Biores. Technology, v. 43, p. 1 - 6, 1993.

MESQUITA, A. C. Uso das técnicas de oxidação química e biodegradação na remoção de alguns compostos recalcitrantes. Dissertação de doutorado, Universidade Federal do Rio de Janeiro, 2004.

MITTELBACH, M. Production and Fuel Properties of Fatty Acid Methyl Ester from used Frying Oil. In : Liquid Fuels from Renewable Sources. Nashville, Tennesse, 1992.

MITTELBACH, M.: The importance of diesel fuel substitutes from non-edible seed oils, janeiro de 2000. Disponível em http://www.balu.kfunigraz.ac.at/~mittelba/ . Acesso em 26 julho 2007.

MITTELBACH, M., TRITTHART, P: Diesel fuel derived from vegetable oils, III. Emission tests using methil esters of used frying oil. v. 65, n 7, p. 1185-1187, 1988.

MONTEIRO, A. S., ASSAKI L. G., DE RARAÚJO, M. J., MITCHEL, A. D., KRIEGER, N., LANZIERI, B. N. Produção de biossurfactantes por Pseudomonas aeruginosa daupe 614. SINAFERM XV Simpósio Nacional de Bioprocessos - Recife, 2005.

MORETTO, E.; ALVES, R.A. Óleos e gorduras vegetais: processamento e análises. Ed. da UFSC, Florianópolis-SC,179 p, 1986.

MORETTO, E., FETT, R. Tecnologia de Óleos e Gorduras Vegetais. São Paulo: Varela, 1998. 150p.

MORETTO, E., FETT, R., GONZAGA, L.V. et al. Introdução à Ciência de Alimentos. Florianópolis: UFSC, 2002. 255Pp.

MORIKAWA, M., DAIDO, H. A new lipopeptide biosurfactant produced by Arthrobacter sp. Strain MIS38. Journal of Biotechnology, p. 6459-6466, 1993. MORIKAW.A, M., HIRATA, Y. IMANAKA, T., A study on the struture-function

ralationship of lipopeptide biosurfactants. Biochimica et Biophysica Acta 1488 p.211-218, 2000.

MULLIGAN, C. N. Environment applications for biosurfactants. Environmental Polluition, 133, 183-198, 2004.

MULLIGAN, C. N., MAHMOURIDES, G., GIBBS, B. F. Biosurfactant production by a chloramphenicol tolerant strain of Pseudomonas aeruginosa. Journal of Biotechnology, Vol. 12, p. 37-44, 1989.

NAVON-VENEZIA, S., ZOSIM, Z., GOTTLIEB, A., LEGMANN, R., CARMELI, S., RON, E. Z., ROSENBERG, E. Alasan, a new biomulsifier from Acinetobacter radioresistents, Applied and Environmental Microbiology, v.61, n. 9, p. 3240- 3244, 1995.

NEUZA, J., SOARES B. B. P., LUNARDI, V. M., & MALACRIDA, C. R. Alterações físico-químicas dos óleos de girassol, milho e soja em frituras. Química Nova, vol. 28, no.6. São Paulo, 2005.

NITSCHKE, M. & PASTORE, G. M. Biossurfactantes: propriedades e aplicações. Química Nova, 25: 772 - 776, 2002.

NORMAS ANALÍTICAS DO INSTITUTO ADOLFO LUTZ (1985). Métodos químicos e físicos para análises de alimentos, 1, 317-319.

OBERBREMER, A., MULLER-HURTIG, R., WAGNER, F. Effect of the addition of microbial surfactant on hidrocarbon degradation in a soil population in a stirred reactor. Applied Microbiology Biotechnology, n. 32, p. 485-489, 1990.

OCHSNER, U. A., REISER, J. Autoinducer-mediated regulation of rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U.S.A. 92: 6424 - 6428, 1995.

OHNO, A., ANO, T., SHODA, M. Production of a lipopeptide antibiotic surfactin by recombinant Bacillus subtilis in solid state fermentation Biotecnology and Bioengineering, v. 47, p. 209-214, 1995.

PALEJWALA, S., DESAI, J. D. Production of an extracellular emulsifier by a Gram- negative bacterium. Biotechnology Letters, 11(2), 115-118, 1989.

PARENTE, E. J. de SÁ. Biodiesel: Uma Aventura Tecnológica num País Engraçado. Fortaleza, 2003. Disponível em: http://www.tecbio.com.br/Downloads/Livro. Acesso em 27 julho 2007.

PARRA, J.L., GUINEA, J., MANRESA, M.A. Chemical characterization and physico- chemical behavior of biosurfactants. Surfactants and Detergents Technical. JAOCS, 66(1), 1989.

PATEL, R.M., DESAI, A.J. Biosurfactant production by Pseudomonas aeruginosa GSB from molasses. Letters in Applied Microbiology, v.25, p.91-94, 1997.

PEIXOTO, N. Processamento de produtos de biomassa de levedura para alimentação humana: potencial, mercado interno e extreno. In: Anais do workshop sobre produção de biomassa de levedura – utilização em alimentação humana e animal, Campinas, SP, p.90-98, 1996.

PELCZAR, M. J. J. CHAN, E. C. S., KRIEG, N. R. Microbiology Concepts and applivations, 5 Ed. New York. Ed. McGraw-Hill, inc. 1993.

PELIZZETTI, E. PRAMAURO, E. Analysis Chemical. Acta, 1985, 169, 1.PERSON, A.; MOLIN, G. Capacity of biosurfactant production og environment Pseudomonas and Vibrionacea growing on carbohidrates. Applied Microbiology and Biotechnology, v. 26, p.439-442, 1987.

PERSSON, A, Osterberg, E., Dostalek, M. Biosurfactant production by Pseudomonas fluorescens 378: growth and product characteristics. Applied Microbiology and Biotechnology, v.29, p.1-4, 1988.

PINNHEIRO-ROBERG, R.A. Aproveitamento de efluentes industriais para a produção de biomassa entomopatogênica à base Bacillus sphaericus. Orientador: Eliana Flavia Camporese Sérvulo. Rio de Janeiro, EQ/UFRJ, Dissertação (Mestrado em Tecnologia de Processos Químicos e Bioquímicos), 2000.

PORTAL DA SECRETARIA DA AGRICULTURA FAMILIAR. Descrição do processo de produção de Soja. Ministério do Desenvolvimento Agrário, Brasília, 2007. Disponível em (www.pronaf.gov.br). Acesso em: 21 março 2007.

PORTER, M.R. Handbook of surfactants. New York: Chapman & Hall, 2a ed., 1994. PROGRAMA COLETA DE ÓLEOS DE FRITURA. Laboratório de Desenvolvimento

de Tecnologias Limpas. Ribeirão Preto – São Paulo, 2006. Disponível em: (http://www.biodieselbrasil.com.br). Acesso em: 27 julho, 2007.

RHAMAN, K. S. M., BANAT, I. M., THAHIRA, J., THAYUMANAVAN, T.; LAKSHMANAPERUMALSAMY, P. Bioremediation of gasoline contaminated soil by a bacterial consortium amended whit poultry litter, coir pith and rhamnolipid biosurfactant. Bioresource Technology, n. 81 p. 25-32, 2002.

RAMANA, K.V. KARANTH, N.G. Factors affecting biosurfactant production using Pseudomonas aeruginosa CFTR-6 under submerged conditions. Journal of Technology and Biotechnology, n 45, p 249-257, 1989a.

RAMANA, K.V. KARANTH, N.G. Factors affecting biosurfactant production using Pseudomonas aeruginosa CFTR-6 under submerged conditions. Journal of Technology and Biotechnology, n 45, p 249-257, 1989 b.

REIS, F. A. S. L., SERVULO, E. F. C., FRANÇA, F. P. Lipopeptide surfactant production by Bacillus subtilis grown on low-cost raw materials. Applied Biochemistry and Biotechnology, 115, 899-912, 2004.

REISER, J. K. A. K., KOCH, K., KAPPELI. O. Structure, properties, and production of biosurfactant, p. 85-97. In J. W. OBRINGER and H. S. TILLINGHAST (ed), Advances in biotechology for aerospace applications. The Portfolio Publishing Co., The Woodlands Tex., 1989.

REISER, J. K. A. K., OCHSNER, U. A., FIECHER, A. Genetic of surface-active compounds. Biosurfactants (surfactant Science series) in: Dekker, (ed.) Nova York. v. 48, p. 231-249, 1993.

RENDELL, N. B., TAYLOR, G. W., SOMERVILLE, M., TODD, H, WILSON, R., COLE J., Characterization of Pseudomonas rhamnolipids. Biochem. Biophys., p.189-1993, 1990.

RIO DE JANEIRO (Estado). Aulas de Pós-Graduação. Alternativas tecnológicas sustentáveis no processamento de óleos vegetais –EQE706. Oleaginosas de interesse comercial e seus usos. Rio de Janeiro, 2006. Disponível em (www.eq.ufrj.br/posgraduacao/aulas/suely/2006/aula2_oleaginosas.ppt). Acesso em: 24 fev. 2006.

RISTAU, E., WAGNER, F. F. Formation of novel anionic trealose tetraesters from Rhodococcus erythopolis growth-limiting conditions. Biotechnology Letters, n. 5, p. 95-100, 1983.

ROBERT, M.; MERCADÉ, M.E.; BOSH, M.P.; PARRA, J.L.; ESPUNY, M.J.; MANRESA, M.A.; GUINEA, J. Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T1. Biotechnology Letters, v.1, n. 2, 871-874, 1989.

ROBERT, M. MERCADÉ,E.; ANDRÉS, C.; ESPUNY, M.J.; MANRESA, M.A.; GUINEA, J. Optimización de la producción de biotensoactivos por Pseudomonas aeruginosa 44T1. Grasas y Aceites, v.42, n.1, p.1-7, 1991.

ROCHA C. SAN-BLAS, F. SAN-BLAS, G. VIERNA, L. Biosurfactant production by two isolates Pseudomonas aeruginosa. World Journal of Microbiology and Biotecnology, v. 8, p 125-128, 1992.

RON, E. Z., ROSENBERG, E. Biosurfactants and oil bioremediation. In: Current Opinion in Biotechnology. Editora Elsevier, v.13, p.249–252, 2002.

ROSEN, M. J. Surfactants and interfacial phenomena. Ed John Wilwy & Sons, USA, 431 p, 1989.

ROSENBERG, E., RUBINOVITZ, C., GOTTLIEB, A., ROSENHAK, S., RON, E. Z. Production of biodispersan by Acinetobacter calcoaceticus A2, 1988.

ROSENBERG, E. & RON E. Z. High and low molecular mass microbial surfactants. Applied Microbiology and Biotechnology, 52, 154 - 162, 1999.

RUFINO, D. R. SARUBBO, L. A., TAKAKI, M. G. Produção de biossurfactante por Candida lipolytica utilizando substrato de baixo custo. SINAFERM XV SimposioNacional de Bioprocessos - Recife, 2005.

SANIBAL, E. A., MANCINI-FILHO, J., Food Ingred. South Am., p. 18-64, 2002.

SANTA ANNA, L. M. SEBASTIAN, G. V. FREIRE D. M. G. MENESES, E. P., ALVES, T. L. M. Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosas PA 1. Applied Biochem. And Biotechnol. p. 90-93, 2001.

SANTA-ANNA, L. M. SEBASTIAN, G. V., PEREIRA J. N., SANTOS A. S., MENEZES E. and FREIRE D. M. G. Production of biosurfactant from Pseudomonas aeruginosa PA1 isolated in oil environmental. Braz. Journal Chem. Eng. v. 19, p. 159-166, 2002.

SANTOS, A. S., SAMPAIO, A. P., VASQUEZ, G. S., SANTA ANNA, L. M., FREIRE, D. M. G., Evaluation of different carbon in nitrogen sources in production of rhamnolipis by a strain Pseudomonas aeruginosa. Applied Bioch.

Belgede T.C. ĠNÖNÜ ÜNĠVERSĠTESĠ SOSYAL BĠLĠMLER ENSTĠTÜSÜ ALGI YÖNETĠMĠ ĠLE DESTĠNASYON MERKEZLERĠNĠN REKABETÇĠLĠĞĠ ARASINDAKĠ ĠLĠġKĠYĠ BELĠRLEMEYE YÖNELĠK BĠR ARAġTIRMA (sayfa 172-178)