BIO 435 BIO 435
ENZYMOLOGY ENZYMOLOGY
1. WEEK
fkiran@science.ankara.edu.tr
Office Tel: 0312 212 67 20-1054
Office
Assoc. Prof. Dr. Fadime KIRAN
-Molecular Biology UnitBuilding A, Z-66
fadime.kiran_ptlab_
GOALS GOALS
To give information systematically and
comprehensively about the enzymes
which are biomolecules used for the
reactions in biological systems and to
show how they take place in metabolic
events and to improve their knowledge
and thinking skills about the use of
enzymes in health, food, textile and
industrial areas.
Week Topics
1. week Overview on Enzymes
2. week Regulation of Catalytic Activity And Energy Conversion
3. week Enzyme Specificity
4. week Mechanisms of Enzymatic Catalysis I 5. week Mechanisms of Enzymatic Catalysis II 6. week Enzyme Kinetics I
7. week Enzyme Kinetics II 8. week Bisubstrate Reactions 9. week Enzyme Inhibitions I 10. week Enzyme Inhibitions II
11. week Examples of Enzymatic Reactions 12. week Regulatory Enzymes
13. week Applications of Enzymes in Industrial Fields I 14. week Applications of Enzymes in Industrial Fields
II
Schedule
Weekly Topics (Content)
Learning Activities Learning Activities
Homework
Presentation (Including Preparation Time)
Brain storming
References References
1) Enzyme Technology, Martin Chaplin and Christopher Bucke, Cambridge University Press, 1990.
2) Lehninger Biyokimyanın İlkeleri, 3. baskıdan çeviri;
David L. Nelson ve Michael M. Cox, Çeviri Editörü: Prof.
Dr. Nedret Kılıç, Palme Yayıncılık, 2004.
3) Principles of Biochemistry, H. R. Horton, L. A. Moran, K. G. Scrimgeour, M. D. Perry, J. D. Rawn, Pearson
Prentis Hall, 2006.
4) Biochemistry, J. M. Berg, J. L.Tymoczko, L. Stryer W.
H. Freeman and Company and Sumanas, Inc, 2005.
5) Color Atlas of Biochemistry, J. Koolman, K. H. Roehm, Georg Thieme Verlag, 2005. Harper’s Illustrated
Biochemistry, R. K. Murray, D. K. Granner, P. A. Mayes, V. W. Rodwell, Lange Medical Books/McGraw-Hill Medical Publishing Division, 2003.
6) Basic Concepts in Biochemistry, A Student’s Survival Guide, H. F. Gilbert, McGraw-Hill Health Proffesions
Division, 2000.
Introduction to
Enzymes
Louis PASTEUR
Wilhelm KUHNE, ENZYMES
Eduard BUCHNER, ZYMASES
Enzymes have been used in biotechnology for millennia
Nomenclature Nomenclature
Enzymes named for reaction they catalyze sucrase breaks down sucrose
proteases break down proteins
lipases break down lipids
Nº Class Reaction Examples
1 Oxidoreductases Oxidation-reduction reactions Glucose oxidase (EC 1.1.3.4)
2 Transferases Transfer of functional groups Hexokinase (EC 2.7.1.2)
3 Hydrolases Hydrolysis reactions Carboxipeptidase A (EC 3.4.17.1)
4 Lyases Addition to double bonds Piruvate decarboxylase (EC 4.1.1.1)
5 Isomerases Isomerisation reactions Malate isomerase (EC 5.2.1.1)
6 Ligases Formation ob bonds (C-C, C-S, C-
O and C-N) with ATP cleavage Piruvate carboxylase (EC 6.4.1.1)
