BIY 435 BIY 435

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III.WEEK

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ENZYME

SPECIFICITY

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• Enzymes are very specific.

• Each enzyme will catalyze only one type of reactions

and often will only work with a specific substrate.

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DIFFERENT TYPES OF ENZYM DIFFERENT TYPES OF ENZYM

SPECIFICITY SPECIFICITY

1. LINKAGE SPECIFICITY

2. GROUP SPECIFICITY

3. SUBSTRAT SPECIFICITY

4. STEREO SPECIFICITY

5. GEOMETRIC SPECIFICITY

6. CO-FACTOR SPECIFICITY

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LINKLINKAGEAGE SPECIFICITY SPECIFICITY

 Amylase can hydrolaze alpha 1-4 glycosidic linkage

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GROUP SPECIFICITY GROUP SPECIFICITY

 Enymes are specific to type of bonds and groups.

 Examples: Pepsin, Trypsin

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SUBSTRAT SPECIFICITY SUBSTRAT SPECIFICITY

 Enzymes are only specific to one substrate and one reaction.

 Example: Lactates act only on lactose

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STEREO SPECIFICITY STEREO SPECIFICITY

 Enzymes are also specific to the optical configurations

 Examples: L amino acid oxidase actes onlt L amino acids

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GEOMETRIC SPECIFICITY GEOMETRIC SPECIFICITY

 Enzyme can act on different substrates having similar

moelcular geometry

 Example: Alcohol dehydrogenase

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CO-FACTOR SPECIFICITY CO-FACTOR SPECIFICITY

 Only correct combinations of substrate-co factor allow

enzymatic reactions