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BIO 435 BIO 435

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BIO 435 BIO 435

ENZYMOLOGY ENZYMOLOGY

VII. WEEK

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Factors Affecting

Enzyme Function

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1. Enzyme concentration 2. Substrate concentration 3. Temperature

4. pH

5. Salinity

6. Activators

7. Inhibitors

(4)

Enzyme concentration Enzyme concentration

 ENZYME =  REACTION RATE

(5)

Substrate

Substrate concentration concentration

 SUBSTRATE =  REACTION RATE

• enzyme is saturated

• maximum rate of reaction

(6)

TEMPERATURE TEMPERATURE

• At high temperatures, enzymes denature, which modifies the active site.

• At low temperatures, enzyme activity is

low due to a lack of energy for the

reaction to occur.

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pH pH

pepsin (stomach) = pH 2-3

trypsin (small intestines) = pH 8

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• Activators – cofactors – coenzymes

• non-protein, organic molecules

• many vitamins

Compounds

Compounds

Referanslar

Benzer Belgeler

When the substrate is bound to the enzyme, catalytic functional groups contribute to the cleavage. and formation of the bond by a group

Reaction rates and equilibria have precise thermodynamic definitions...  Substrate concentration affects the rate

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Allosteric enzymes exist in either exist in either an active or inactive state. an active or

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Some regulatory enzymes undergo reversible

 KİMOTRİPSİN: AROMATİK aminoasitlerin katıldığı peptid bağlarını KARBOKSİL uçtan keser... SUBSTRAT SPESİFİKLİĞİ SUBSTRAT SPESİFİKLİĞİ. 

 1) Enzyme Technology, Martin Chaplin and Christopher Bucke, Cambridge University Press, 1990. Cox, Çeviri