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Biochemical Tests Mobility Test
Many bacteria are active against environmental factors.
The movement takes place via flagellum
Monotric flagella: A single flagella (Vibrio cholerae)
Peritric flagella: A large number of flagella in many parts of the cell (E. coli)
Lofotric Flagella: More than one flagella at one or both ends (Pseudomonas species).
Amphitric flagella: One flagella at each end (Spirillum volutans)
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Selective Media: A medium that promotes the development of a particular microorganism and prevents the growth of others. Differential Media: A medium used to distinguish differences
crystal violet in the medium prevent the growth of Gram (+) bacteria and are used as selective indicator in the medium. When lactose is used, a red color is formed due to the pH
1) For fermentation test, phenol red is placed on four nutrient broth media containing glucose, sucrose,. lactose
Fermentation Tests - Triple Sugar Iron (TSI) Agar Test.. TSI agar is especially used to identify Gram (-) bacteria such as
At the end of the experiment, it is obtained whether glucose is used in fermentative way, organic acids are.. formed in the medium and
If the microorganism has the amylase activity, it will break down starch molecules and use glucose when added to starchy agar medium. In this case,
If microorganism having the gelatinase enzyme forms a gelatinase zone in the gelatin nutrient medium. If there is liquefaction in the medium:
Antibiogram tests are often used to determine antibiotics and effect doses that can be used to prevent the growth or growth of
