Prof. Dr. Sevgi ERTUĞRUL KARATAY
Media Used in Microbiology
The media to be used in microbiological studies can be selected according to the following criteria.
• Metabolism of microorganism
• pH
• Redox balance of culture
• Respiratory type (aerobic, anaerobic)
• etc.
Media Used in Microbiology Culture media can be used for
•Enrichment of microorganims
•To obtain pure colonies (Purification)
•Select a specific microorganism and suppress the development of others
•To distinguish certain types of microorganisms
•Enumeration of the microorganisms
Media Used in Microbiology
Media according to Oxygen Needs of Microorganisms
•Aerobic
•Anaerobic
•Obligat anaerobe
•Facultative anaerobe
•Microaerophilic
Microbiological Media
Media of microorganisms according to optimum pH range
Many bacteria are grown between pH 6.5-7.5 Many yeast and fungi pH between 5 and 6
Acidophilic microorganisms, acidic conditions etc.
Media Used in Microbiology Media Types
General Purpose Media
Contains peptone, yeast extract, sugars, etc. media, although the exact composition of all components is
unknown.
Defined medium
Media in which all components are known.
Media Used in Microbiology Media Types
General Purpose Media: Media that supports the development of many different microorganisms. This type of medium contains
sheep blood, peptone etc. elements. (Example: Mueller Hinton Agar, Sheep Blood Agar)
Specific Media: Media that supports the development of a certain type of microorganism
Selective Media: A medium that promotes the development of a particular microorganism and prevents the growth of others.
Differential Media: A medium used to distinguish differences between microorganisms with observable biological differences
(color, colony character, etc.).
