3. Alt Sektörler ve D›fl Ticaretteki Geliflmeler
3.13. Tar›m ve Ormanc›l›kta Kullan›lan Makineler
Com base nos resultados desta tese, conclui-se que:
1. O fotossensibilizador azul de orto toluidina é eficaz na redução de S. mutans. Contudo, a produção de oxigênio singlete não é o parâmetro mais relevante a considerar ao avaliar a atividade antimicrobiana da terapia fotodinâmica contra S.
mutans.
2. Há similar difusão do azul de orto toluidina em diferentes níveis de desmineralização da dentina. Assim, há importância da influência do perfil de penetração do fotossensibilizador e subsequente sucesso em ensaios clínicos da terapia fotodinâmica no campo da cárie dental.
3. A terapia antimicrobiana fotodinâmica foi altamente eficaz em matar S. mutans presentes em biofilmes formados in vitro. No entanto, não foi capaz de alterar a expressão de genes de virulência gtfB, gtfC e gbpB. Isto é especialmente importante quando se considera que a terapia não elimina todos os microorganismos presentes no biofilme e que a presença de bactérias residuais em biofilmes irradiados com maior expressão destes genes pode resultar na indução de uma maior virulência. Finalmente, a indução da expressão de genes relacionados com o stress oxidativo, tais como luxS, devem ser investigados de modo a esclarecer a influência da terapia fotodinâmica antimicrobiana na organização do biofilme e virulência após o tratamento.
4. Houve diminuição significativa da microbiota cariogênica em dentina de lesões de cárie após terapia antimicrobiana fotodinâmica, evidenciando que a proposta da terapia fotodinâmica é plausível e poderá promover novos conceitos terapêuticas no tratamento da cárie. Desta forma, os estudos disponíveis justificam os esforços na investigação para melhorar esta terapia.
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APÊNDICE A
INFORMAÇÕES E CONSENTIMENTO PÓS-INFORMAÇÃO PARA