魚油添加全反式視網酸對 C57BL/6J 小鼠脂質代謝的影響
Effects of Fish Oil Supplement with All-Trans Retinoic Acid on Lipid
Metabolism in C57BL/6J Mice
中文摘要
本研究係探討魚油中添加全反式視網酸 (all-trans retinoic acid；ATRA)對小鼠體
內脂質代謝產生的影響。以40 隻 C57BL/6J 小鼠為研究對象並隨機分為四組：
魚油組 (FO)、大豆油組 (SO)、魚油添加 ATRA 組 (FR)、大豆油添加 ATRA 組 (SR)，四組油脂均給予 27% (w/w)，FR 和 SR 額外添加 66.7 mg/kg diet 的 ATRA。 實驗期四個月後，分析肝臟和血液中三酸甘油酯 (TG)、總膽固醇 (TC)、低密度 脂蛋白膽固醇 (LDL-C)、高密度脂蛋白膽固醇 (HDL-C)、游離脂肪酸濃度 (NEFA)，Fatty acid synthase (FAS)、Acyl-CoA oxidase (ACO)、Acetyl-CoA carboxylase (ACC)、catalase 等酵素活性，與肝臟 PPAR (peroxisome proliferator activated receptor)δ、RAR (retinoic acid receptor)α蛋白表現量，並將肝臟和白色
脂肪組織取出做H&E 染色。結果顯示魚油能顯著降低體重、肝脂質及 FAS 活性， 並增加PPARδ蛋白質表現。而全反式視網酸的添加對於降低體重、血脂及肝脂 質具有加成之效果。由上述結果推論，魚油能較大豆油增加PPARδ蛋白表現， 而全反式視網酸的給予能更有效降低血液和肝臟脂質並增加RARα表現，加速 脂質分解、減少脂質合成反應之進行。 英文摘要
The aim of this study was to assess the fish oil plus all-trans retinoic acid (ATRA) on lipid metabolism in mice. Forty C57BL/6J male mice were divides into fish oil (FO), soybean oil (SO) , fish oil plus ATRA (FR), soybean oil plus ATRA (SR) four groups. After four months, liver and serum triglyceride, total cholesterol, LDL-C, HDL-C, free fatty acids were analyzed, liver PPAR (peroxisome proliferator-activated receptor) delta, RAR (retinoic acid receptor) alpha protein expression and FAS (fatty acid
synthase), ACO (acyl-CoA oxidase), ACC (acetyl-CoA carboxylase), catalase enzymes activity were also measured. The results demonstrated fish oil had lower concentration in the liver and serum triglyceride, total cholesterol, and FAS activity also significantly decreased. The liver PPAR delta expression was higher in FO group. And oil plus all-trans retinoic acid had more efficiency in lower body weight, liver and blood lipids. Supplement with all-trans retinoic acid also increased retinoic acid receptor α protein expression. In conclusion that fish oil can increase the PPAR delta expression, moreover fish oil plus ATRA can be more effectively to reduce blood and liver lipids. This will help to reduce weight gain and prevent obesity.
