高劑量β－胡蘿蔔素在攝取不同油脂大白鼠體內之利用率及對脂質
代謝的影響
The availability and effects on lipid metabolism of large dose β-carotene in rats fed different fat diets.
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中文摘要
本研究的目的是探討高劑量 β-胡蘿蔔素在攝取不同油脂大白鼠體內之利率，進 而研究 β-胡蘿蔔素在脂質代謝上所扮演的角色。將 80 隻 Wistar 品系雄性大白 鼠(重約 250 公克) ，隨機分成八組，以 β-胡蘿蔔素 (0.2 ％ ，給予或不給予)、
脂質種類 ( 黃豆油或豬油 )及脂質濃度(5 ％或 15 ％) 作 2×2×2 factorial 的設
計，於第3 週及第 6 週分別宰殺大白鼠，取血液及肝臟，測定血清與肝臟中三酸
甘油酯及總膽固醇含量，血清中並分析低密度脂蛋白膽固醇及高密度脂蛋白膽固 醇含量，同時利用 HPLC 分析血清及肝臟中β-胡蘿蔔素、維生素 A 含量。結 果顯示：食物攝取量無顯著差異；不論是否給予 β-胡蘿蔔素，高黃豆油組有顯 著較高的最終體重及餵食效率( P <0.01)。脂質分析方面，黃豆油／給予β-胡蘿 蔔素組之血清三酸甘油酯高於黃豆油／不給予β-胡蘿蔔素組，豬油組有相反的 結果( P=0.0007)；第 3 週之肝臟三酸甘油酯，高黃豆油組顯著高於其它(P<0.05)，
而第 6 週發現給予 β-胡蘿蔔素會降低肝臟三酸甘油酯 (P=0.0051) ；血清中總 膽固醇、高密度脂蛋白膽固醇及低密度脂蛋白膽固醇，於第 3 週無顯著差異，
第6 週則是高油組高於低油組(P<0.05)，且血清β-胡蘿蔔素濃度與高密度脂蛋白 膽固醇／低密度脂蛋白膽固醇比率呈負相關 (r=-0.35，P=0.0374)。第 3 週之肝 臟膽固醇在高油時，黃豆油組高於豬油組，低油時則相反 (P=0.0001)，第 6 週時，
高黃豆油組顯著高於其它組(P <0.005)。血清及肝臟中 β-胡蘿蔔素有相同的趨 勢，第 3 週時，高油組顯著高於低油組( 血清，P=0.0028；肝臟，P=0.0001)，
且高豬油組高於高黃豆油組(p<0.05)。β-胡蘿蔔素的給予對於血清維生素 A 在
第3 週時無影響，第 6 週時則會降低；肝臟維生素 A 則會因β-胡蘿蔔素的給予
而上升。
英文摘要
The purpose of this study was to investigate the availability and the effects on lipid metabolism of large dose β-carotene (BC) in rats fed different fat diets. Male adult Wistar rats(N=88 ) were randomly divided into eight groups in a ２×２×２factorial design for three or six weeks. These diets varied in BC content (0.2 ％, with or without), lipid type (soybean oil or lard) and lipid level (5 ％ or 15 ％). Analytical items included serum tri- glyceride(STG), serum total cholesterol(SC), high density lipo- protein cholesterol(HDL-C), low density lipoprotein cholesterol (LDL-C), liver
triglyceride(LTG) and liver total cholesterol(LC). HPLC was used to analyze BC and vitamin A concentrations. The re- sults showed that 15 ％ soybean oil group had higher final body weight and feed efficiency (p<0.0100). The effects on lipid meta- bolism were as follows: soybean oil/with BC group had higher STG than soybean oil/without BC group,and the lard groups had reverse result (p=0.0007); 15％
soybean oil group had highest LTG amomg eight groups after three-week feeding, and it was lowest when given BC after six-week feeding (p=0.0051); 15％ oil/fat groups had higher SC, HDL-C and LDL-C than 5％ oil/fat groups after six- week feeding (p<0.05), in addition, serum BC had negative correl- ation (r=-0.35, p=0.0374) with HDL-C/LDL-C ratio; 15％ soybean oil group had higher LC than 15％ lard group after three-week feeding, 5％ oil/fat groups had reverse result (p=0.0001), but 15％
soybean oil group had highest LC after six- week feeding (p< 0.005). Serum and liver BC showed similar results that 15％ oil/ fat groups had higher BC than 5％ oil/
fat groups (serum, p=0.0028 ; liver, p=0.0001), and 15％ lard group had higher BC than 15％salad oil group (p<0.05). With BC groups had lower serum vitamin A than without BC groups after six-week feeding. Liver vitamin A increased with the presence of BC (p<0.05).
