高劑量 β －胡蘿蔔素在攝取不同油脂大白鼠體內之利用率及對脂質代謝的影響
中文摘要
本研究的目的是探討高劑量 β- 胡蘿蔔素在攝取不同油脂大白鼠體內之利率，進而研究 β- 胡蘿蔔素在脂質代謝上所扮演的角色。將 80 隻 Wistar 品系雄性大白鼠 ( 重約 250 公 克 ) ，隨機分成八組，以 β- 胡蘿蔔素 (0.2 ％ ，給予或不給予 ) 、脂質種類 ( 黃豆 油或豬油 ) 及脂質濃度 (5 ％或 15 ％ ) 作 2×2×2 factorial 的設計，於第 3 週及第 6 週 分別宰殺大白鼠，取血液及肝臟，測定血清與肝臟中三酸甘油酯及總膽固醇含量，血清 中並分析低密度脂蛋白膽固醇及高密度脂蛋白膽固醇含量，同時利用 HPLC 分析血清 及肝臟中 β- 胡蘿蔔素、維生素 A 含量。結果顯示：食物攝取量無顯著差異；不論是 否給予 β- 胡蘿蔔素，高黃豆油組有顯著較高的最終體重及餵食效率 ( P <0.01) 。脂質 分析方面，黃豆油／給予 β- 胡蘿蔔素組之血清三酸甘油酯高於黃豆油／不給予 β- 胡蘿 蔔素組，豬油組有相反的結果 ( P=0.0007) ；第 3 週之肝臟三酸甘油酯，高黃豆油組顯 著高於其它 (P<0.05) ，而第 6 週發現給予 β- 胡蘿蔔素會降低肝臟三酸甘油酯 (P=0.0051) ；血清中總膽固醇、高密度脂蛋白膽固醇及低密度脂蛋白膽固醇，於第 3 週無 顯著差異，第 6 週則是高油組高於低油組 (P<0.05) ，且血清 β- 胡蘿蔔素濃度與高密度 脂蛋白膽固醇／低密度脂蛋白膽固醇比率呈負相關 (r=-0.35 ， P=0.0374) 。第 3 週之 肝臟膽固醇在高油時，黃豆油組高於豬油組，低油時則相反 (P=0.0001) ，第 6 週時，
高黃豆油組顯著高於其它組 (P <0.005) 。血清及肝臟中 β- 胡蘿蔔素有相同的趨勢，第 3 週時，高油組顯著高於低油組 ( 血清， P=0.0028 ；肝臟， P=0.0001) ，且高豬油組高 於高黃豆油組 (p<0.05) 。 β- 胡蘿蔔素的給予對於血清維生素 A 在第 3 週時無影響，第 6 週時則會降低；肝臟維生素 A 則會因 β- 胡蘿蔔素的給予而上升。
The availability and effects on lipid metabolism of large dose β-carotene in rats fed different fat diets.
英文摘要
The purpose of this study was to investigate the availability and the effects on lipid metabolism of large do se β-carotene (BC) in rats fed different fat diets. Male adult Wistar rats(N=88 ) were randomly divided into eight groups in a ２ × ２ × ２ factorial design for three or six weeks. These diets varied in BC content (0.2
％ , with or without), lipid type (soybean oil or lard) and lipid level (5 ％ or 15 ％ ). Analytical items inc luded serum tri- glyceride(STG), serum total cholesterol(SC), high density lipo- protein cholesterol(HDL- C), low density lipoprotein cholesterol (LDL-C), liver triglyceride(LTG) and liver total cholesterol(LC). H PLC was used to analyze BC and vitamin A concentrations. The re- sults showed that 15 ％ soybean oil g roup had higher final body weight and feed efficiency (p<0.0100). The effects on lipid meta- bolism were a s follows: soybean oil/with BC group had higher STG than soybean oil/without BC group,and the lard grou ps had reverse result (p=0.0007); 15 ％ soybean oil group had highest LTG amomg eight groups after thre e-week feeding, and it was lowest when given BC after six-week feeding (p=0.0051); 15 ％ oil/fat groups had higher SC, HDL-C and LDL-C than 5 ％ oil/fat groups after six- week feeding (p<0.05), in addition, s erum BC had negative correl- ation (r=-0.35, p=0.0374) with HDL-C/LDL-C ratio; 15 ％ soybean oil gro up had higher LC than 15 ％ lard group after three-week feeding, 5 ％ oil/fat groups had reverse result (p=0.0001), but 15 ％ soybean oil group had highest LC after six- week feeding (p< 0.005). Serum and liv er BC showed similar results that 15 ％ oil/ fat groups had higher BC than 5 ％ oil/ fat groups (serum, p
=0.0028 ; liver, p=0.0001), and 15 ％ lard group had higher BC than 15 ％ salad oil group (p<0.05). With BC groups had lower serum vitamin A than without BC groups after six-week feeding. Liver vitamin A inc reased with the presence of BC (p<0.05).
