Veterina~y Faculty, Department of Protozoology, Medical Arthropodology and Control
~f
Parasitic Diseases, Universi~y~f
AnkaraProf Dr. Mihri Mimioğlu
THE PRESENCE OF EİMERİA BAREILLYİ (GiII, Chhabra
and Lall, 1963) IN BUFFALO IN TURKEY
Fahri Sayın
*
Suınrnary : This is a reı:ort whieh is eonccrning with the presence of E. bareillyi (Cill, Chhabra and Lall, i963) in water buffalo (Bubalus bubalis) in Turkey. The oocysts of this species were found in the intestinal content of i out of 50 (2%) buffalocs originated from the province of Adapazarı. The morphological characteristics of sporulated oocysts ~ere discus'ci in the paper.
Türkiye'de Mandalarda Bulunan Eimeria bareillyi (GiII, Chhabra
ve Lall, 1963) üzerinde İncelemeler
Özet; Önceki ara5tırmalarımızla Türkiye'de, mandalarda, varlığını bildirdiğimiz
i i adet Eimeria türünden ba5ka, Eimeria bareillyi adı verilen diğer bir türün de bulunduğu anla51lmıştır. Bu türün oocystlerine, Adapazarı'ndan Ankara mezbahasına kesim için geti-rilen 50 mandadan i tanesinin (%2) dışkısında raslanmıştır.
E. bareillyi'nin oocystcleri armut biçiminde, sarımsı esmer renktedirier. Cidarları düz, çift katlı ve ı,2 mikron kalınlığa sahiptirler. Ince kutuplarında mikropil vardır ve bunun genişliği 4--:i mikrondur. Ooeystlerin uzunluğu 24-29, genişliği 15-19 mikrondur. Bunh'.r 22 C. de 3 günde sporlanmaktadırlar. Ooeystler~ ve sporoeystlere ait protoplazma kalıntısı mevcuttur. Sporoeystler 15-17 mikron uzunlukta ve 7-8 mikronkalınlıktadırlar (Şekil I).
Introduction
BufTalo coccidia has called attention recentIy to investigators in several countries. The surveys of faecal samples for coccidia oocysts were carrİed out and the species which were found in Russia19, India 2' 4' ı2' ı s and Turkey ı6 were aıready reported.
Eimeria bareillyi was found initially in India 4. The oocysts of this species was obtained in the intestinal content of water bufTaloes
• Profcssor at The Department of Protozoology, Medical Arthropodology and Control of Parasitic Diseases, Veterinary Faeulty, University of Ankara, Turkey.
The Presence of Eimeria Bareillyi in Buffalo 30
(Bubalus bubalis) in. Bareilly, Izatnagar, Nagla and Utter Paradesh regions2'4. it was observed in 5
%
of 305 buffaloes in these placesı.In addition an oocyst form from the faccal sampk of buffalo was described in Pakistan ıand the name of Eimeria bubalis was uScd
for ipo. This species showed similar morphological characteristics • to Eimeria bareilfyi. Therefore E. bareilryi and E. bubalis were considered as one and the same species8•
The purpose of the present paper is to report the occurrence of E. bareil(yi in water buffalo in Turkey.
Material and Methods
This study is based on the examination of sporulated oocysts of E. bareil(yi observed in the intestinalcontent of i out of 5° water
buffaloes brought from Adapazarı to Ankara meat plant to slaughter. The faecal samples wc re obtained froin rectums of apparently healthy animals. After washİng and straining of the faecal samples, oocysts were conc~ntrated by centrifugal flotation methodwith Sheat-her's suger solution for discovery. The sample which contained the oocysts of E. bareilryi was mixed with 2.5 % Potassium Dichromate solution and placed in a thin layer in a Petri dish at room temperature to permit the. oocysts to sporulatc. The sporulated oocysts were concentrated by the method which was mentioned above prior to the study of their morphological characteristics. They were examined with Olympus microscope (Photomax) equipped with apochromatic plain o~jectives. The pİcture of the sporulated oocyst was drawn by camera lucidia.
Results
The oocysts of E. bareilryi occurred i out of 50 (2 %) faecal
samp-les taken from apparently healthy animals. The number of the " oocysts which present in each gram of facces was 50.
The oocysts of E. bareilryi are typically piriform with bluntly truncate smail end (fİg. ı). Oocyst wall İs smooth, homogenus, ycl-lowish to darkish brown and composed of two layers. Outer layer is colorless and inner one darkish brown İn color. it is 1.2 microns
thİck except at the micropylar end where it is thinner. There is a mic-rapyle at the smail end. it İs 4-5 microns in width. Twenty oocysts wc re 24-29 by '15-i9 (average 27 by 17) microns. A few smail granules
40 I' ••lır; Sayııı
oacysts The oocysts required 3 days to comple!c thcir sporulations at 22 C. Oocyst rcsiduum was present, but oocyst polar granulc absent.
Sporocysts were alongate ovoid and had stieda body at their smaıı ends. They were 15-17 by 7-8 (average 16 by 7) microns in dimen-sion. Sporoeyst residuum was present. Sporozoites were b;.:.nana sha-ped, each with a refractile spherical globule at its large end and sometimes one or two smailer globules. .
Discussion
Twelve species of bovine coccidia were found in cattle and water buffalo in Turkey (16,17). One more species, E. bareillyi, recovered in buffiılo is added to the list by this study.
E. baıeillyi may be eonfused with E. bukidnonensis on account . of İts pear like oocysts. But our own obsen:ation showed that E.
bareil(yi and E. bukidnonensis were quite distİnct. The ooeyst of E. bukidnonensis is larger than that of E. bareil(yi and has a thiek, radial strieted, singIe-rather than a double-- layered walı. Besides the spo-rulation time of E. bukidnonensis is longer than that of E bareill)ıi .1'
h' 7'8'9' 10'iı' i3' 17. It may be also eonfused ""ith E. bOliis on
ae-eount of the siınilarity on the dimensions and double layered waıı of their oocysts. But E. bovis is distinguished quite easy by its ooeyst whieh is broadly ovoid in shapc and does not have an ooeyst
resi-cl
IIum J' 5' b'7' 8' 9' ıo'ıi' ı .:' [.1'ı4' i 6' i7~iS.Our material agrees with the descriptions givcn Lıy Gill et al4
and Bhatia et al2 from India.
References
i. Abdusselaın, M. and Rauf, A. (1956): Coccidiosis of domestic
buffalo (Bubalus bubalis). Proc. 8th Pakistan Sei. Co ne Med. 1956: 6.
2. Bhatia, B. B., Pande, B. P., Chauhan, P. P. S. and Arora,
G. S. (1968): A stud.Y on the sporulatea oOCJ'stsof twelue Eimerian species in lndian b4falo (Bubalus bubalis). Acta Vet. Hungar. 18:115-133. 3. Davies, S. F., joyner, L. P. and Kendall, S. B. (1963):
Coc-ci(i/osis. Oliver and Boyd, Edinburgh X
+
264 pp. .4. Gill, B. S., Chhabra, M. B. and Lall, N. B. (I 963): A new spe-cies of coccidium-Eimeria bareiııyi n. sp. from buffaloes. Arch. Pro-tistenk. 106: 571-574.
The Prc,cııcc of Eimeria Barcillyi İn Ruffal" 11
unsolved Logan, 5- Hammond, D. M. (1964): Goecidio~is of eaUle,.. some
problems. 30th Fae. Honor Leet., Utah State lJniv., Utah, 36 pp.
6. Joyner, L. P., Narton, C. C., Davies, S. F. M. and Watkins,
C. V. (1966): The speeies of eoeeidia oecurring in eatile and sheep in the South West of England. Para'litology 56: 531-441.
7. Lee, R. P. and Armour, J. (1959): The eoecidian OO~ystsof Ni-gerian eatile. British Yet.
.1.,
iIS: 6 17.8. Levine, N. D. and Ivens, V; (197°): The eoeeidian parasites (Protozoa, Sporozoa)
0/
ruminants. Illinoıs Bıologieal Monographs 44, Univ. of Illinois Press, 278 rp.9. Levine,N. D. and Ivens, V. (I 967): The sporulated ooej'st.>of Eimeria illinoisensis n. sp ana ~/ other speeies of Eimeria of the OX.
.1.
Protozool, 14: 351-360.10. Levine, N. D. (I96I): Protlizoan parasites oj domestie animals and of man~ Burges, Minneapolis, 4i2 PJ?'
iI. Nyberg, ~. A. and Hammond, D. M. (I 965): Deseription
~f
the sporulated ooeysts and sporozoits of four speeies of bovine eoeeidia ..1.
Parasit. 51: 669-673.12. Patnaik, M. M. (I 965): On the eoeeidian infeetiiJns of buffalo ealves. A study
d
the oocysts. Agra Unıv ..1.
Res. (Sci), 13 :239-256. 13. Pelerdy, L. P. (I 965): Goeeidia and Goeeidiosis. Akad, Kiado,Budapest. 657 pp.
14. Pellerdy, L. P. (1963),: Gatalogue of Eimeriidea (Protozoa: Sporo-zoa). Akad. Kioda, Budapest, 160 pp.
15. Ray, H. N. and Mandal, L. N. (1961): On a new eoecidium, E. ovoidalis n. sp., from buffalo ea(f and its experiment al transmission to a eow eaif. 48th Indian Sei. Cong. Part 3: 41i (Zoology and
Entomolgy section) .
. 16. Sayın, F. (I 968): The sporulated oocysts of Eimeria ankarensis n. sp, and of other .ıpeeies of Eimeria of Buffalo in Turk~y and transmissi-on offour speeias of Eimeria from bulfalo to eow ealves. Ankara Univ. Yet. Fak. Derg., 15: 3-4, 282-3°0.
17. Sayın, F. (I97°): The speeies of Eimeria oecurring in eatile in Turkey. Ankara Univ. Yet. Fak. Derg. 17: 3, 310-326.
18. Supperer, R. (1952): Die Goeeidien des Rindes in Osterreieh. Oestr. Zoo!. 3: 591-601.
anımaux domestiques Belg. Med. Trop.,
Fuhri Sayın
19- Yakimoff, V. L. (1933): La coccidiose des
dans L' A;;:erbaidjan (Transcaucasia). Ann. Soc. 13: 93-13°.
20. Yasin, S. A. and Abdusselam, M. (I958) :Recent Contributions to Veterinary Parasitolog.y in Pakistan. Bull. üfr. Intern. Epizoot. 49 bis: 481-492•
Received 4.4. 1973
