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MICROBIAL BIOTECHNOLOGY

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BIO 440

MICROBIAL

BIOTECHNOLOGY

(2)

Vector and Host Systems Used in Cloning

(3)

-Hosts for Cloning Vectors Bacterial hosts

Eukaryotics

(4)

- Specialized Vectors and Types

• Plasmid vectors (pUC19 plasmid cloning vector)

• Shuttle Vectors

• Expression (expression) vectors (T7 expression system)

(5)

- Specialized Vectors and Types

• Bacteriophage M13 vector

• Eukaryotic viral vectors

(6)

- Specialized Vectors and Types

• Ti plasmids

• Fusion and Cosmid vectors

(7)

- Specialized Vectors and Types

• Artificial bacterial chromosome vector (BAC) used in sequencing

(8)

- Specialized Vectors and Types

• Artificial yeast vector (YAC) containing foreign DNA used in sequencing

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