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Animal
Reproduction
Science
j o ur na l ho me p ag e :w w w. e l s e v i e r . c o m / l o c a t e / a n i r e p r o s c i
The
effect
of
maternal
nutritional
status
during
mid-gestation
on
placental
characteristics
in
ewes
U.
Sen
a,
E.
Sirin
b,
M.
Kuran
a,∗aOndokuzMayisUniversity,AgriculturalFaculty,Kurupelit,TR55139Samsun,Turkey
bAhiEvranUniversity,AgriculturalFaculty,Asikpasa,TR40100Kirsehir,Turkey
a
r
t
i
c
l
e
i
n
f
o
Articlehistory:
Received15February2012
Receivedinrevisedform
22November2012
Accepted29November2012
Available online 7 December 2012 Keywords:
Maternalnutrition
Mid-gestation Placenta Cotyledon
Lambbirthweight
a
b
s
t
r
a
c
t
Theaimofthisstudywastodeterminetheeffectsofmaternalnutritionalstatus dur-ingmid-gestationonplacentalcharacteristicsinewes.Timeofestrusof3–5yearsold Karayakabreedeweswassynchronizedandmatingwasmonitoredtodeterminetheday0 ofgestation.Theeweshadsimilarbodyweights(47.8±0.7kg)andloineyemusclevalues (thickness;20.9±1.0mmandfatthickness;4.7±0.5mm)atmating.Theeweswere allo-catedintotwotreatmentgroupsatday30ofgestation;under-fed(UF;n=12)andwell-fed (WF;n=13)groups.TheewesinUFgroupwerefedwithadiettoprovide50%oftheirdaily requirementfromday30today80ofgestationand100%oftheirdailyrequirementduring therestofthegestationperiod.TheewesinWFgroupwerefedatleast100%oftheirdaily requirementthroughoutgestation.ThesingletonbearingewesintheUFgrouphadalesser (P<0.05)placentalweight(354.1comparedwith378.3g),averagecotyledonweight(1.50 comparedwith1.82g)andlambbirthweight(3.8comparedwith4.2kg)thansingleton bearingewesintheWFgroup.Therewerepositivecorrelationsbetweenplacentalweight andlambbirthweight(r=.469;P<0.05),placentalweightandaveragecotyledonweight (r=.695;P<0.01),averagecotyledonweightandlambbirthweight(r=.742;P<0.01)and placentalefficiencyandcotyledondensity(r=.853;P<0.01)forewesinWFgroup. Addi-tionally,thepatternofweightgain/losswasdifferent(P<0.05)betweenthetwogroups. EwesinUFgrouplostbodyweightprogressivelyfromday30ofgestationuntilday80. Theresultsofpresentstudyshowthatunder-feedingofewesduringmid-gestationmay causeaninsufficientplacentaldevelopmentandhencealterfetaldevelopmentresultingin areducedbirthweightfromsingletonpregnancies.
© 2012 Elsevier B.V. All rights reserved.
1. Introduction
Maternal nutrient intakeduring gestation in various speciesaffectsthesizeanddevelopmentofboththe pla-centaandthefetus(Redmeretal.,2004).Survivalofthe fetusisaffectedespeciallybysufficiencyoftheplacenta (Mellor and Stafford, 2004). Furthermore, the size and
∗ Correspondingauthorat:OndokuzMayisUniversitesi,Ziraat
Fakul-tesi,Kurupelit,TR55139Samsun,Turkey.Tel.:+903624576086;
fax:+903624576034.
E-mailaddress:mkuran@omu.edu.tr(M.Kuran).
nutrienttransfer capacityof theplacentaplay a central roleindeterminingtheprenatalgrowthtrajectoryofthe fetusandresultinginlong-termprogrammingeffectson birthweight,postnatalgrowth,viability,productivityand long-termhealthoftheoffspring(McMullenetal.,2005). Maternal nutrient restrictionduring gestationhas been reportedtocauseareduction inproliferationof placen-tal mass, as well as impaired umbilical blood flows. A poorlydevelopedplacentarestrictsnutrientand respira-torygasessupply,leadingtofetalhypoxia,hypoglycemia or low nutrientuptakes, asymmetricorgan growthand reducedfetal growthrates(Wuetal.,2006;Ocaket al., 2009).Indeedtheimportanceoftheplacentalcirculationis
0378-4320/$–seefrontmatter © 2012 Elsevier B.V. All rights reserved.
exemplifiedbythecloserelationshipsamongfetalweights, placentalweightsandutero-placentalbloodflowsin nor-malandgrowthrestrictedpregnancies(Greenwoodetal., 2000; Redmer et al., 2004). Severe restrictions of sub-stratesupplymayresultinmaternal,placentalandfetal adaptationstothealteredpre-natalenvironment,which allowssurvival oftheoffspring, oftenattheexpenseof thenormalfetaldevelopment(Quigleyetal.,2008).There isincreasingevidencefromepidemiological and experi-mentalstudiesthattheprogrammingoffetaldevelopment maybeparticularlysensitivetomaternalnutrientstatus duringtheperi-conceptionperiod(Robinsonetal.,1997; Quigleyetal., 2005;Hernandezetal.,2009; Igwebuike, 2010).Thus,itisobviousthatamountofmaternal nutri-tionaffectingplacentalvasculardevelopmentwillhavea dramaticimpactonfetalgrowthanddevelopment.
Attachmentoffetalcotyledonstothematernal carun-clesoccursbetweendays25and30ofgestationinsheep, and growthrestriction duringthis period can limit the eventualnumberofplacentomesthatformtheplacenta (Igwebuike, 2010). The majority of placental growth in termsofmassandnetcellularproliferationoccursduring thefirsttwo-thirdsofgestationinsheep(Redmeretal., 2004; Igwebuike,2010). Also, the placentaachieves its maximumweightbyday90ofgestationandnochangein tissuedrymattercontentoccursthereafter(Samminetal., 2009;Igwebuike,2010).Duringthisperiod,thefetushas only10%ofitseventualbirthweightbythetimethe pla-centahasreacheditsmaximumsize(Redmeretal.,2004; Samminetal.,2009).Inthiscontext,approximately90% offetal growthoccursduringthelast thirdof gestation whenafterthecompletedevelopmentoftheplacentain sheep(Redmeretal.,2004).Theindicationisthatplacental sizeandevenfunctionlateringestationmaybe substan-tiallyinfluencedatthisearlystageinsheep(Igwebuike, 2010).
Theconsequencesofnutritionstatusinthefirsthalfof pregnancyforruminantplacentalgrowthhavebeenrarely studied.However,availableevidencesupporttheideathat placentalgrowth,andbyimplication,fetalgrowthismost vulnerabletomaternalnutritionalstatusduringthe peri-implantation period and thefirst trimester of gestation whenrapidplacentaldevelopmentoccurs(Wuetal.,2004). Theusualmatingperiodofewesinmostpartsoftheworld coincideswiththeautumnandearlywintermonthsduring whichnutrientintakeofpregnantewesmaybelessthanat othertimesoftheyearduetopoorrangelandquality.Thus itcanbepostulatedthatmostofsheepinmostpartsofthe worldexperiencemalnutritionduringthegestationperiod which maycausealtered placental development result-inginlesserbirthweightsandpooreradultperformance postweaningduetonutritionalprogrammingeffects dur-inggestation.Theaimofthepresentstudy,therefore,was todeterminetheeffectsofmaternalnutritionalstatus dur-ingmid-gestationonplacentalcharacteristicsinsingleton bearingewes.Itiswellknownthatlittersizehasa signif-icantroleintheplacentalsize(Konyalietal.,2007;Ocak etal.,2009).TheKarayakabreedsheepusedinthisstudy isagoodmodelforassessingnutritionalinfluenceson pla-centalcharacteristicsbecausethisbreedisknownforlow prolificacy(approximately1.1)thustheinfluenceoflitter
sizeonplacentalcharacteristicswasminimizedbyusing thisbreed.
2. Materialsandmethods
The experimental procedures were approved by the Local AnimalCareand EthicsCommitteeof Gaziosman-pasaUniversity,Tokat,Turkeyensuringcompliancewith ECDirective86/609/EECforanimalexperiments.Thestudy wasconductedwithinthenormalseasonalbreedingcycle of ewes in Turkey (Septemberto March). Experimental animals were of the Karayaka breed,3–5 years of age, andmaintainedattheSheepFarmofGaziosmanpasa Uni-versity,Tokat,Turkey(40◦31N,36◦53Eand650mabove thesealevel).Timeofestruswassynchronizedbyuseof intravaginalspongescontaining30mgflugestoneacetate (Chronogest;Intervet,Turkey)for12daysfollowedbyan intramuscularinjectionof1mlofPGF2␣(Dinolytic;5mg
PF2␣/ml,Pharmacia,Belgium).Forty-eighthourslater,the eweswereintroducedtoKarayakarams(approximately oneramtoevery10ewes),andthematingwasmonitored todetermineday0ofgestation.Theeweshadsimilarbody weightsandeyemusclevalues,measuredbyanultrasonic linearprobeatmating.
Eweswerehousedinindividualpensatday55of gesta-tion.Themeanbodyweightsofewesweredeterminedat days30,45,60and80ofgestationandatbirth. Addition-ally,lointhicknessandfatthicknessvaluesofeyemuscle weredeterminedat days30 and80 ofgestationand at birth.Theeweswereallocatedintotwotreatmentgroups; under-fed(UF;n=12)andwell-fed(WF;n=13)groups.The ewesinWFgroupwerecomposedoftwosub-groups;ewes offeredwithadiettobefedeitheradlibitum(sub-group1; n=4;consumed175%ofthedailyrequirements)or100% oftheirdailyrequirements(sub-group2;n=9)fromday 30today80ofgestation.Thesetwosub-groupswere com-bined, becausethere werenodifferencesbetweenewes whenallvariablesweremeasured.TheewesinUFgroup were fedwith a diet tomeet 50%of thedaily require-mentsfromday30today80ofgestation.Duringtherest ofthegestationperiod,theewesinbothgroupswerefed withadiettomeet100%ofthedailyrequirements.The dailyDMrequirementsofewesthroughoutthisstudywere calculatedonanindividualewebodyweightbasis.Diets were composedof concentrate (89.5%DM, 23.4% crude proteinand 10.9MJME/kgDM),and goodqualityalfalfa hay(88.4%DM,16.8%crudeproteinand8.2MJME/kgDM). Theamountsof concentrateandhayconsumptionwere 991.3±77.1and975.9±29.1g/day/eweinWFgroup,and 200.0±0.2and468.4±8.9g/day/eweinUFgroup, respec-tively.Dietsweregivendailyintwoequalmealsat08:30 and16:30.Eweswereweighedatintervalsofaboutevery 2weeksandthequantityofdietadjustedforweightgain andforweightloss.Waterandmineralswerefreely avail-ablethroughoutthestudy.Allewesgavebirthtosingletons exceptfourewesinWFgroup.Theseeweswereexcluded fromthestudytoavoidlittersizeeffectsonplacental char-acteristics.
Birthweightandthesexoflambswererecordedwithin 12hafterlambing.Eachewewaslefttodelivertheplacenta naturally and placentas were collected from singleton
pregnanciesimmediatelyafterdelivery(approximately2h afterthebirthofthelamb);carewastakentoensurethat anyplacentalweightstakenwereofthetotalplacentawith anyfluidbeingremovedbeforeweighting.Thenumbers andweightsofcotyledonswerealsocounted,determined andrecorded.Placentalefficiency(definedasgramoflamb produced pergramofplacenta)wascalculatedforeach ewe.Thecotyledondensitywascalculatedasthenumber ofcotyledonsingramplacentalweight.
Theeffectofmaternalnutritionduringmid-gestation onlambbirthweight,placentalcharacteristicsandother variables wereinvestigated usingacompletely random-izeddesignbyusingGLMprocedureofMinitab(Minitab, 1998).Thesexoflambswasusedasacofactorinthemodel toadjustthebirthweightandtheplacental characteris-tics. Relationshipsbetween theplacental characteristics weredeterminedwithaPearsoncorrelationanalysisatthe 95%confidenceinterval.TheFisherztransformationtest wasappliedtoassess thesignificance ofthedifferences between correlation coefficients of two groups. Signifi-cantdifferencesbetweenmeansweretestedusingTukey’s multiplecomparisontestsandresultswerecomputedas mean±SEM.
3. Results
The changes in maternal body weights (BW) from mating to birth in experimental ewes in WF and UF groupsarepresented inFig.1.Alleweshad similarBW (47.8±0.7kg) at mating and there were no significant differences between ewes in WF (48.5±0.9kg) and UF (48.2±0.7kg)groupsatday30ofgestation.Thepatternof weightgain/losswas,however,different(P<0.05)between thetwogroups.EwesinUFgrouplostweightprogressively fromday30untilday80(P<0.05)ofgestation.Fromday 80ofgestation,followinginitiationoffeedingof100%of thedailyrequirements,ewesgainedweightsuchthatat theendofgestationtheBWwasnotdifferentfromthatof ewesinWFgroup(WF;49.4±0.9kg,UF;48.9±0.7kg).
Thechangesinlointhicknessandfatthicknessvalues oftheloineyemusclefrommatingtobirthinWFandUF
Changes in body weight (kg)
-6 -4 -2 0 2 4 6 8 10 WF UF
*
*
*
Periods of gestation 30 45 60 80 BirthFig.1.Thechangesinmaternalbodyweightsfrommatingtoday30,
45,60,80ofgestationandbirthinewesinWFandUFgroups.Asterisks
indicatedifferencesbetweentreatmentgroups(P<0.05).UF=under-fed
group,WF=well-fedgroup.
Changes in loin thickness (mm)
-3 -2 -1 0 1 WF UF
*
30 80 Birth Periods of gestationFig.2.Thechangesinloineyethicknessvaluesfrommatingtoday30,80
ofgestationandbirthinewesinWFandUFgroups.Asteriskindicates
differencesbetweentreatmentgroups(P<0.05).UF=under-fedgroup,
WF=well-fedgroup.
ewesarepresentedinFigs.2and3,respectively.Allewes hadsimilarloineye(20.9±1.0mm)andfat(4.7±0.5mm) thicknessvalues atmating. Also, there wereno signifi-cantdifferences in loin eye and fat thickness values of eye muscle between WF (loin thickness; 21.0±1.1mm and fatthickness; 4.7±0.5mm)and UF(lointhickness; 20.9±1.0mmandfatthickness;4.5±0.7mm)ewesatday 30ofgestation.Therewasadecrease(P<0.05)inloineye andfatthicknessvaluesinUFewesatday80ofgestation, afterwhichtherewasnofurtherchangeuntilbirth.Loineye andfatthicknessvaluesweresimilarthroughoutgestation intheWFgroup.
Unadjustedand adjusted meansfor length of gesta-tion,lambbirthweightsand placentalcharacteristicsof ewesinWFandUFgroupsarepresentedinTable1.The sexoflambshadnoeffectonanyvariablesstudied.The lengthofgestationwasnotsignificantlydifferentbetween UFandWFewes.Therewas,however,adecreaseinthe birthweightofthelambsbornfromewesinUFgroup com-paredwiththelambsfromewesinWF(P<0.05).Number ofcotyledons,cotyledondensityandplacentalefficiency werenotsignificantlydifferentbetweenUFandWFewes. However,thereweredecreasesintheplacentaland aver-agecotyledonweightsinUFewescomparedtoWFewes (P<0.05).
Changes in fat thickness (mm)
-0,6 -0,3 0 0,3 WF UF
*
Periods of gestation 30 80 BirthFig.3.Thechangesinfatthicknessvaluesfrommatingtoday30,80
ofgestationandbirthinewesinWFandUFgroups.Asteriskindicates
differencesbetweentreatmentgroups(P<0.05).UF=under-fedgroup,
Table1
Unadjustedandadjustedmeans,accordingtolambsex,forlengthofgestation,lambbirthweightsandsomeplacentalcharacteristics(means±SEM)of
ewesinWFandUFgroups.
Traits Sub-group1 Sub-group2 Treatmentgroupsc
WF UF
Unadjusted mean
Adjustedmean Unadjusted
mean Adjusted mean F/M(n) 2/2 4/5 6/7 6/6 LG(day) 146.7±0.83 147.9±0.58 147.1±0.68 146.6±0.83 148.9±0.58 147.8±0.7 BW(kg) 4.28±0.039 4.21±0.013 4.24±0.027a 4.22±0.033a 3.74±0.119b 3.73±0.141b PW(g) 404.3±15.4 379.8±9.3 387.3±10.28a 387.8±11.94a 352.8±13.33b 348.7±15.54b TCN(n) 71.28±0.980 69.03±0.670 69.69±0.850 70.16±0.982 71.75±3.925 70.22±4.620 ACW(g) 1.92±0.497 1.90±0.312 1.91±0.381a 1.90±0.051a 1.57±0.136b 1.62±0.176b PE 11.13±0.947 11.51±0.803 11.35±0.859 11.27±0.295 10.75±1.481 10.84±0.455 CD 0.188±0.042 0.187±0.035 0.187±0.038 0.188±0.005 0.202±0.045 0.199±0.015
Meansinrowswithdifferentletters(a,b)aredifferentatP<0.05.
SEM=standarderrorofmean,F=female,M=maleLG=lengthofgestation,BW=lambbirthweight,PW=placentalweight,TCN=totalcotyledonnumber
perplacenta,ACW=averagecotyledonweight,PE=placentalefficiency,CD=cotyledondensity.
c TheewesinWF(well-fed)groupwerecomposedoftwosub-groups;ewesofferedwithadiettobefedeitheradlibitum(sub-group1;n=4;consumed
175%oftheirdailyrequirement)or100%oftheirdailyrequirements(sub-group2;n=9)fromday30today80ofgestation.TheewesinUFgroup(under-fed;
n=12)werefedwithadiettomeet50%oftheirdailyrequirementsfromday30today80ofgestation.
Table2
Pearsoncorrelationcoefficients(95%confidenceintervals)ofplacentalcharacteristicsandlambbirthweightofewesinWFgroups.
Traits PW PE CD ACW BW CN 0.053(−0.609,0.715) −0.053(−0.715,0.609) 0.286(−0.349,0.921) −0.323(−0.951,0.305) −0.059(−0.721,0.603) PW −0.868**(−1.197,0.538) −0.889**(−1.192,−0.585) 0.695**(0.217,1.172) 0.469*(−0.117,1.055) PE 0.853**(0.506,1.199) −0.459(−1.048,0.130) −0.077(−0.738,0.584) CD −0.799**(−1.198,−0.399) −0.470(−1.055,0.115) ACW 0.742**(0.297,1.186)
BW=lambbirthweight;PW=placentalweight;PE=placentalefficiency;CN=cotyledonnumber;CD=cotyledondensity;ACW=averagecotyledonweight.
* P<0.05.
** P<0.01.
Table3
Pearsoncorrelationcoefficients(95%confidenceintervals)ofplacentalcharacteristicsandlambbirthweightofewesinUFgroups.
Traits PW PE CD ACW BW CN 0.032(−0.672,0.736) −0.076(−0.778,0.626) 0.759**(0.300,1.217) −0.539*(−1.132,0.054) 0.019(−0.685,0.723) PW −0.797**(−1.222,−0.371) −0.619*(−1.172,−0.065) 0.287(−0.387,0.961) 0.021(−0.683,0.725) PE 0.445(−0.185,1.075) 0.004(−0.700,0.708) 0.565*(−0.016,1.146) CD −0.645*(−1.183,−0.106) −0.034(−0.738,0.670) ACW 0.435(−0.199,1.069)
BW=lambbirthweight;PW=placentalweight;PE=placentalefficiency;CN=cotyledonnumber;CD=cotyledondensity;ACW=averagecotyledonweight.
* P<0.05.
** P<0.01.
Pearsoncorrelationcoefficientsofplacental traitsfor ewesinWFandUFgroupsarepresentedinTables2and3, respectively.Inbothgroupstherewerenegative correla-tionsbetweenplacentalweight and placentalefficiency (r=−.868; and r=−.797; P<0.01), placental weight and cotyledon density (r=−.889; P<0.01 and r=−.619; P<0.05), and cotyledon density and average cotyle-don weight (r=−.799; P<0.01 and r=−.645; P<0.05) for ewes WF and UF groups, respectively. There were positive correlations between placental weight and lamb birth weight (r=.469; P<0.05), placental weight and average cotyledon weight (r=.695; P<0.01), aver-age cotyledon weight and lamb birth weight (r=.742; P<0.01) and placental efficiency and cotyledon den-sity (r=.853; P<0.01) for ewes in WF group. As can be expected there were positive correlations between cotyledon number and cotyledon density (r=.759;
P<0.01) and negative correlations between average cotyledon weight and cotyledon number (r=−.539; P<0.05) for ewes in UF group. There were positive correlationsbetweenplacentalefficiencyandlambbirth weight(r=.565;P<0.05)intheUFgroup.Therewereno significantcorrelationcoefficientsforvariablesintheUF andWFgroups.
4. Discussion
Thepresentstudydemonstratesthatamountof nutri-tionduringmid-gestation(from30to80days)influences placental weight, average cotyledon weight and birth weightinsingletonbearingewes.Additionally undernour-ishedeweshada lesserbody weight,loineyethickness andfatthicknessvaluethanwell-fedewesbyday80of gestation,reflectingthenutritionaltreatments.
The placentais theorganthrough whichrespiratory gases,nutrients,andwastes areexchangedbetweenthe maternal and fetal systems (Wu et al., 2006).The pla-centaalsoistheprimarycontributortotheintrauterine environment for growthand developmentof the fetus. Although the fetal genome plays an important role in growthpotentialinutero,thereisevidencesuggestingthat theintrauterineenvironmentisanimportantdeterminant offetalgrowth(Wuetal.,2006;Igwebuike,2010).Placental andtheumbilicalcordsizearecloselyrelatedtofetal devel-opmentandgrowthrate(GodfreyandBarker,2000;Wu etal.,2006;Ocaketal.,2009).Maternalnutrition,however, mayimpactplacentaldevelopment,growthandvascular beddevelopment(angiogenesis),therebyaffecting utero-placentalbloodflowandnutrientuptakebythefetus,all ofwhichmayplayanimportantroleinfetalgrowthand metabolism,aswellasbirthweight(Redmeretal.,2004). Experiments in sheepshowedthat amountof maternal nutritioninearlyandmidgestationcanexertmajoreffects onthegrowth,developmentandweightoftheplacenta andtherebyalterfetaldevelopment(Clarkeetal.,1998; Osgerbyetal.,2002;Faheyetal.,2005;McMullenetal., 2005)assupportedbytheresultsofthepresentstudy.
Although there are differences between treatment groups in terms of birth weight, placental weight and averagecotyledonweight,itisinterestingthatplacental efficiency andcotyledon densitydidnot differbetween ewes inUF andWF groups.Thismay beduetosimilar directionalchangesinbirthandplacentalweightsinboth groups.Infact,therewereapproximately9%decreasesin placental and 12%in birth weight.Theresultsobtained from the present study show that amount of maternal nutritionbetweenday30 andday80 ofgestation influ-encedplacental andaveragecotyledonweights,but not totalcotyledonnumberperplacenta.Theseobservations arein agreementwiththeviewthatmaternal nutrition during gestation can affect placental development and thusreduceplacentalweightanddecreaseplacentalsize (Clarkeetal., 1998;Osgerbyetal.,2002; Redmeretal., 2004; Wu et al.,2006).Additionally, suchan alteration in placentaldevelopmentmayhavedramatic functional consequences in fetal development.The reason for the reducedlambbirthweightinundernourishedewesmight beinadequateplacentalgrowthanddevelopment.Lambs fromundernourishedewesduringmid-gestationmayhave pooreradultperformance duetothegenomic program-mingeffectsofnutritionduringgestation(Redmeretal., 2004;Wuetal.,2006).Indeedit hasbeenreportedthat thelambsfromtheUFgrouphadalesserweaningweight, alteredcarcasscompositionandmusclefibertypes follow-ingfattening(Kuranetal.,2007,2008).
Ocak et al. (2009) reported that placental weight waspositivelycorrelatedwithaveragecotyledonweight, cotyledonnumberandcotyledondensityinsheep.Konyali etal.(2007)reportedsimilarfindingsforgoats.The cor-relationsobservedinthepresentstudybetweenplacental characteristicsinthewell-fedgroupsupportstheviewthat theinfluenceof maternalnutritiononplacental charac-teristics resultsinaltered birthweight.It wasobserved thatincreasedplacentalweightasa resultofamountof nutritioncausesanincreaseinaveragecotyledonandlamb
birthweight.Therewasalsoapositivecorrelationbetween averagecotyledonandlambbirthweightinwell-fedewes. The negative correlationbetween placental weight and efficiencyobservedinthepresentstudysupportsthe find-ingsofOcakandOnder(2011)forgoats.
ItisinterestingthattheewesintheUFgroupshowed compensatorygrowthfromday80tobirth,andthe nutri-tionalinfluenceonliveweight,loineyethicknessandfat thicknesswasnotevidentbetweencontrolandtreatment groupsbythetimeofbirth.Howeverdifferencesin placen-talweightsbetweenewesinUFandWFgroupsremained atbirthrepresentinglongtermeffectswhendietsdonot meet nutritionalrecommendations onplacental growth anddevelopment.Feedingdietsthatdo notmeet nutri-tional recommendations during mid-gestation caused a decreaseinbodyweight,loineyethicknessandfat thick-nessinthepresentstudy.Thisresultindicatesthatbody reservesinpregnantewesdependonamountofnutritional intake.Osgerbyetal.(2002)reportedthatundernourished ewes(onthe70%diet)duringdifferentperiodsofgestation (45,90,135dayofgestation)hadlesserbodyweightsand bodyconditionscorescomparedtothewell-fedewes(on the100%diet).
5. Conclusions
Theresultsof present studyshowedthat amountof maternalnutritionduringmid-gestationimpacts placen-talgrowthanddevelopmentofsingletonbearingewesas indicatedbyplacentalandaveragecotyledonweight caus-ingalteredfetaldevelopmentandhenceresultinginlesser lambbirthweightswhichmayaffectadultperformanceof lambs.Thesefindingsmaybeimportanttodevelopnovel nutritionalstrategies duringcriticalperiodsof gestation whichmayyieldpreferableadultperformanceandmeat quality.
Acknowledgement
The authors acknowledge the financial support by TUBITAK(TBAG-U/148)tocarryoutthisstudy.
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