In-vitro Embryo Production (IVP)
Method to obtain transferable embryos out of a body, at the laboratory conditions
Requires;
- Ovum Pick-Up (OPU)
- In-vitro Maturation (IVM) - In-vitro Fertilization (IVF) - In-vitro Culture (IVC)
Ovum Pick-Up
is collection of immature oocytes from
slaughtered animal’s ovary by aspiration or slicing,
live animals by surgical (Laparotomy) laparoscopy
?ultrasound guided?
Animal recovery Repeatability
Oocyte quality Oocyte number
Ovum Pick-Up
LOPU
IVM, IVF, IVC
• In-vitro Maturation (IVM)
• Maturate the oocyte to metaphase II stage that is fertilizable stage of the oocyte evolution
• In-vitro Fertilization (IVF)
• Fertilize the mature oocyte with capasitated semen to create an embryo
• In-vitro Culture (IVC)
• Culture the embryo until it reaches to morula or blastocyst stage
