Embryo Transfer
The way to produce large number of offspring from selected dams Accelerate selection
Decrease generation interval
Embryo Transfer
Selection of donor and recipients
Superovulation of donor and synchronization of recipients
Insemination of donor
Collection and evaluation of embryos Embryo transfer
2 mg 2 mg
1,5 mg 1,5 mg
1 mg 1 mg
0,5 mg 0,5 mg Progesteron
sponge
FSH injections
Progesteron
sponge PMSG injection
500 IU
Sponge removal
Superovulation
48 hours
Embryo Collection
Surgical
(Laparotomy)
Long preparation period
Operative trauma, adhesions Repeatability is limited
Non-surgical
(Laparoscopy or Trans-cervical passage) Relatively short preparation
Fast donor recovery
Minimize stress and drug Repeatablility
Transfer
Transfers may be done at 4-6th day after inseminations.
Embryos must be in morula or balstocyst stage
Hints to attent
• PMSG may result in lower numbers of embryo than FSH.
• Non-surgical embryo collection sould be performed because of their multiple adventages.