INVESTIGATION OF TOXICITY OF VARIOUS
NANOPARTICLES ON CORD ORIGINATED MESENCHYMAL
STEM CELLS
OBJECTIVE: Some of the commonly used stem cell components are
bone marrow, adipose tissue, cord blood and cord matrix. Isolated cord derived using various methods (cord matrix ) high proliferation potential of mesenchymal stem cells can be applied to toxicity studies. The purpose of this study is to investigate the effect of the nanoparticles such as titanium dioxide, titanium silver, silver and zinc on mesencyhmal stem cells obtained from cord matrix in order to be used in tissue engineering.
METHODS: The methods used in the experiments were; isolation
(explant), microscopic, culture, cell count and vibiality analysis 3 - (4,5-dimethyltriazole-2-yl) -2,5-diphenyltetrazolium bromide (MTT) and flow cytometric.
CONCLUSİON: Due to the lowest nanoparticle toxic effect, titanium silver with the highest EC50 was determined as the most suitable application for the cord matrix originated mesencyhmal stem cells. The non-toxic concentrations of titanium, titanium silver, silver and zinc nanoparticles to be used as medicine, vaccine and in different fields of tissue engineering.
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5th INTERNATIONAL CONGRESS OF MOLECULAR MEDICINE, 20– 22 MAY 2015, IZMIR, TURKEY
MELIKE ERSOZ1, ADIL ALLAHVERDIYEV2
1 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Bilim University, Istanbul, Turkey 2 Department of Bioengineering, Faculty of Chemical and Metallurgical, Yıldız Technical University, Istanbul, Turkey
RESULTS: As a result of the microscopic and flow sytometric
analysis, surplus number of healthy mesencyhmal stem cells were isolated from cord matrix using explant method in a short period of time without using enzymes (Figure 1,2). MTT results revealed that titanium, titanium silver, silver and zinc nanoparticles overdose has a toxic effect on cord originated mesencyhmal stem cells as well as decreasing the proliferation. in cord originated mesencyhmal stem cells titanium EC50 is 20,58, titanium silver EC50 is 33,3, silver EC50 is 12,11 and zinc EC50 is 3,1 (Figure 3).
Figure 1: Mesencyhmal stem cells were isolated from cord matrix using explant method 96-hour microscopic image (20x)
Figure 2: A result of flow sytometric analysis mesencyhmal stem cells were isolated from cord matrix using explant method
Figure 3: In cord matrix originated mesencyhmal stem cells titanium, titanium silver, silver and zinc nanoparticles EC50
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