Sonuç

Amplifikasyon, sekans analizi, gerçek zamanlı PZR analizi, tek nokta mutasyon analizi ve genetiği değiştirilmiş organizma tayini gibi DNA temelli moleküler çalışmalarda en önemli basamağı saf ve kırılmamış DNA elde edilmesidir. Bu çalışmada, gıda endüstrisinde kullanılan ticari olarak temin edilen pamuk yağı örneklerinden dört farklı yöntem ile DNA izolasyon çalışması gerçekleştirildi. CTAB-hekzan-kloroform, CTAB- merkaptoetanol,CTAB ve kit yöntemiyle elde edilen DNA’ların konsantrasyon ve saflık değerleri spektrofotometri ile belirlendi. Bu yöntemlerle izole edilen DNA’ların PZR’de etkin bir şekilde amplifikasyona maruz kalıp kalmama durumları değerlendirildi. Bunun için pamuğa (Gossypium) özgü acyl taşıyıcı protein 1 kodlayan gen bölgesine (acp 1)

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göre tasarlanan primerler ve genetiği değiştirilmiş gene ait 35S promotor bölgesine göre tasarlanan primerler ile amplifikasyon reaksiyonu gerçekleştirildi.

Sonuç olarak, CTAB temelli manuel yöntemlerle izolasyon işleminden sonra ortamdan uzaklaştırılamayan polisakkarit ve diğer moleküllerin amplifikasyonu engellediği sonucuna varıldı. Kit yöntemiyle yapılan izolasyonda ise yeterli miktarda DNA elde edilemediği ve bu nedenle amplifikasyonun başarılı şekilde yapılamadı değerlendirildi. DNA’ların etanol ile temizlenmesinden sonra daha saf hale geldiği spektral olarak belirlendi ve CTAB yöntemiyle izole edilen DNA’larda amplifikasyon reaksyionu bazı örnekler için gerçekleştirildi.

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ÖZGEÇMİŞ

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Adresi: Ziyagökalp Mah.865 Sok. Mimoza Apartman No:35 Kat 1 merkez /KARAMAN

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Lise: Pendik Rauf Denktaş Lisesi

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Yüksek Lisans: Karamanoğlu Mehmetbey Üniversitesi/ Biyomühendislik Ana Bilim Dalı

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