Glucocorticoid regulation of the activation state of drugs secreted proteoglycan astrocytes and impact the ability of nerve fiber growth of
Abstract
Central nervous system axonal regeneration after injury limited capacity, often causing permanent loss of neurological function in patients, which exists in the extracellular matrix (extracellular matrix, ECM) of the chondroitin sulfate proteoglycan (chondroitin sulfate proteoglycans, CSPGs) in the injury will be re- activated astrocytes secreted and present in the injured parts of the formation of glial scar tissue around is considered one of the factors inhibiting axonal
regeneration. Currently used to treat central nervous system injury with glucocorticoid steroids neuroprotective effect, but such drugs will affect the activation of astrocytes and the ability to secrete extracellular matrix is not clear.
In this study, using cultured astrocytes and the early generation of excitatory amino acid glutamate to similar objects R, S α-amino-3-hydroxyl-5-
methylisoxazole-4-propionic acid (AMPA) to simulate the in vivo stimulation Star injuries like glial cells, astrocytes were observed in this case secretion of
extracellular matrix components and whether the ability Hui by glucocorticoids 药 物, methylprednisolone (MP) the influence, and simultaneously to study injury of glial cells secretion of extracellular matrix components for neurite growth.
The results show that MPcan indeed inhibit the addition of chondroitin sulfate (chondroitin sulfate, CS) GAG (glycosaminoglycan, GAG) and hylauronan-binding class of CSPGs in the injured model performance, but forsmall leucine-rich type The CSPGs are effect was not significant. The sensing molecule gene regulation studies have shown that MPfor hylauronan-bindingclass of CSPGs of inhibition may be mediated through glucocorticoids receptor (glucocorticoid receptors, GRs) role, and astrocytes to MPmedium can promote neurite growth may be through theinhibition of ECM in CSPGs. Another observation of different types of CSPGs for dorsal root ganglion cells in the growth of axons, the results show that CSPG GAG and core protein in the nerve axons for the different effects on the growth and its regulation may be through a different mechanism of action turn, these results can be speculated that MPdrugs involved in the regulation of central nervous system injury and reorganization of extracellular matrix in order to affect the growth of injured axons.
English Abstract
