Mother of Pearl toxin chicken cell biology
AbstractChicken Mother Pearl (Jequiriti Bean) for the legumes (Leguminosae) Acacia (Abrus precatorius L.
) Seeds by Sepharose 4B and Sephadex G-100 chromatography purification of two toxic proteins, respectively, Abrus agglutinin (AAG) and Abrin. To investigate the AAG, Abrin and "Angelica Long Hui Wan," the impact on the immune system, is used Rosette assay, hemagglutination test and indirect immunofluorescence test method, the number of cells were determined antigens, antibody titer and helper T cell /
inhibitory T cells (TH / TS) ratio. The results showed that the three had immune enhancement for mice. AAG, Abrin and "Angelica Long Hui Wan" Jieke increase the number of mouse cells of antigens and TH / TS ratio, as antibody titers, in this study found no increase. For the mother of pearl on the chicken further explore the toxic protein, is trying to manufacture monoclonal antibodies against Abrin to simplify the purification steps Abrin and AAG, and to improve the purity of AAG. Found to 100ng Abrin with Freund's adjuvant, interval of two weeks, three times by intraperitoneal dosing, then 1000ng Abrin strengthen the injection, the serum antibody titers by enzyme immunoassay (ELISA) measured up to 104 or more. To the rural state of immunized spleen cells and NS-1 cell line, using 1ml 50% PEG (MW 1500) integration, 440% of the fusion rate obtained by ELISA screening
, About 5% of fusion cells secreting anti Abrin antibody.
