Mother Pearl toxin-induced chicken tumor necrosis factor study
Abstract
BCG, OK-432 and other immune enhancer that can stimulate the activation of macrophages in the anti-cancer effect has been
affected by today's pharmaceutical industry attention. Sloan Kettering Institute in 1975, the United States of Dr. LJ Old and EA Carswell, etc., the first report of TNF (Tumor Necrosis Factor; TNF) may cause necrosis of tumor cells, and then the tumor disappeared.
Chicken Mother Chu Hemagglutinin (Abrus Agglutinin; AAG) and mother of pearl chicken toxic protein (Abrin; Ab), in the 1970s Jibei proven anti-cancer effect, its mechanism, in addition to direct cytotoxicity, the can still strengthen the immune function, we can see the chicken for a special anti-cancer mother of pearl material, but the toxicity of too much, has not yet been used in clinic. No species of TNF-specific, and not toxic to normal cells, where it can be induced by TNF chicken mother beads used in clinical practice will contribute to the development of anticancer drugs and the use of plant resources.
The study consists of three parts: 1. By in vivo bioassay, the mother of pearl chicken toxin-induced activity of tumor necrosis factor, and the minimum conditions of tumor necrosis. 2. Cytotoxic test by external inspection, to explore the induction mechanism of TNS, and TNS activity titer, while setting an economic saving of the
experimental method. 3.TNS on liver function and the effect of peripheral blood leukocyte classification.
The results revealed that AAG can be when the first stimulus material priming agent, Ab can be when the second stimulus (eliciting agent) induced TNF, tumor necrosis its lead to optimal conditions for AAG 200ng/mouse, Ab 20ng/mouse, Ab for 2 hours after the blood samples to 0.4ml of serum, intravenous injection of mice to fear cancer, can be the most significant tumor necrosis, and no kind of specificity of TNF on the liver and peripheral blood cells in the Mu no
significant difference in the percentage of neutrophils, but 50 percent of its in vitro cytotoxic titer was 26LU/ml.
In this study, AAG, Ab induced TNF substances, but this substance is reported in the TNF and Old for the same material, required further purification and biochemical confirmation.
