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Single Radial Haemolysis Assay

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Academic year: 2021

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(1)
(2)

Antibody diffusion within a gel for the

determination of antibodies that might be present in analysed sera.

The haemolysis, mediated by complement and induced by the antibody-antigen complex,

(3)

Usage areas

Virus identification (rarely)

(4)

What to do for the SRH test

Agar: 1-2% Noble agar or agarose

Fresh blood of Complement from Vertebrates

Sensitized erythrocyte: By processing some of the chemical substances (chromium chloride, potassium periodate)

specific antigens of the virus transferred to erythrocytes.

(5)

Application of Test

Pour; agar, complement and sensitized erythrocytes

To the petri dish and incubate 1 night to freze.

Put suspicious serum and (+) And (-) sera in the holes on agar incubate 1 night.

The next day, the petri dishes taken from the refrigerator are kept at room

(6)
(7)

Around (-) control the well HEMOLYSIS should not occur. Around(+) Control well HEMOLYSIS should occur.

Antigen specificity in suspect serum sample

If there is an antibody; Antigen-antibody complex occurs and complement binds to that complex

And Lysing the erythrocytes. As a result

(8)

Evaluation

Referanslar

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