HEALTH MICROBIOLOGY

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BIO 431

HEALTH MICROBIOLOGY

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B- DIAGNOSTIC MICROBIOLOGY AND IMMUNOBIOLOGICAL METHODS

Diagnostic methods used in health microbiology:

I-Classical diagnostic methods

II- Molecular diagnostic methods

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• The microbiologist plays a critical role in the

diagnosis of agents that cause infectious

diseases. This area of microbiology is called

diagnostic or clinical-microbiology.

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• Clinical laboratories can develop, isolate and

diagnose the most common pathogenic

bacteria within 48 hours after sampling.

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• Immunological and molecular methods can

also be used in the diagnosis of many

pathogens without culturing the organism.

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• These methods are particularly important in

the diagnosis of viral and protozoal infections,

which are difficult to diagnose due to

difficulties in cultivating the agent.

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I- Classic diagnostic methods for microbial development

• Isolation of Pathogens from Clinical Samples

• Diagnostic Methods Due to Microbial Development

• Antimicrobial Drug Sensitivity Tests

• Security in Microbiology Laboratory

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I- Immunology and Clinical diagnostic methods

• Immunoassays for Infectious Diseases

• Polyclonal and Monoclonal Antibodies

• In vitro Antigen-Antibody Reactions: Serology

• agglutination

• Fluorescent Antibodies

• Enzyme Bound Immunosorbent Assay and Radioimmunassay

• Immunoblot Methods

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AREAS USING ELISA

• Diagnosis of disease-causing pathogensDiagnosis of disease-causing pathogens

• Pregnancy test (Human Corionic Gonodotropin = HCG)Pregnancy test (Human Corionic Gonodotropin = HCG)

• Drug detectionDrug detection

• Searching for gene cloning productsSearching for gene cloning products

• Searching for pathological proteinsSearching for pathological proteins

• Diagnosis of autoimmune diseasesDiagnosis of autoimmune diseases