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中文摘要  雞母珠(

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雞母珠毒蛋白之細胞生物學研究

中文摘要

雞母珠( Jequiriti Bean )為豆科( Leguminosae )相思樹( Abrus precatorius L.

)之種子,經 Sepharose 4B 及 Sephadex G-100 chromatography 純化出兩種毒 蛋白,分別為 Abrus agglutinin ( AAG )及 Abrin 。為探討 AAG , Abrin 及“當歸龍薈丸”對免疫系統的影響,乃利用 Rosette 檢測法,血球凝集試 試驗及間接免疫螢光染色法,分別測定抗原反應細胞數目,抗體力價及輔助 性 T 細胞/抑制性 T 細胞( TH/TS )之比值。結果顯示,三者對於小白鼠 均有免疫增強作用。 AAG 、 Abrin 及“當歸龍薈丸”皆可提高小白鼠之抗 原反應細胞數目及 TH/TS 比值,至於抗體力價,在本研究中未發現提高。為 對雞母珠毒蛋白做進一步探討,乃嘗試製造抗 Abrin 之單株抗體,以簡化 Ab rin 及 AAG 之純化步驟,並提高 AAG 之純度。發現以 100ng Abrin 配合 Fr eund's 佐劑,間隔二週,連續腹腔投予三次,再以 1000ng Abrin 加強注 射,血清中抗體力價經酵素免疫分析法( ELISA )測定,可達 104 以上。

將上鄉述免疫過之脾細胞與 NS-1 細胞株,利用 1ml 50 % PEG(MW 1500) 融

合,可得到 440 %之融合率,經 ELISA 篩選後,約有 5 %融合細胞會分泌

抗 Abrin 抗體。

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