Abstracts / Journal of Biotechnology 305S (2019) S12–S32 S19 weights. The lipid accumulation can be strongly dependent on the
availability of phosphorus in the growth media. While the effect of phosphorus on lipid accumulation in algae has been addressed in the scientific literature, the role of phosphorus in lipid accumula-tion mechanisms in fungi is unknown.
In this paper, we study the effect of twelve growth condi-tions, namely six phosphorus concentrations using two nitrogen sources (yeast extract and ammonium sulphate), for ten different oleaginous Zygomycetes fungi grown in Duetz-MTPs. The single-step biochemical phenotyping of fungal biomass was performed by FTIR spectroscopy and the analysis of fatty acid profiles by GC-FID chromatography.
https://doi.org/10.1016/j.jbiotec.2019.05.076 OP-APR12-A13
Biotechnological tools for introgression breeding for adaptation of crops to climate change
J. Prohens∗, P. Gramazio, M. Plazas, A. Fita, E. García Fortea, G. Mangino, A. Arrones, G. Villanueva, M.D. Lerma, D. Alonso, A. Rodríguez Burruezo, S. Vilanova
COMAV, Universitat Politecnica de Valencia, Valencia, Spain
Climate change will increase the occurrence of biotic and abi-otic stresses in agriculture. Among crop wild relatives (CWRs) it is common to find adaptive traits for tolerance to stresses of inter-est for genetically improving crops for developing new resilient cultivars. We have proposed a new strategy denominated “intro-gressiomics” which is aimed at the systematic development of materials that contain introgressions from wild species in a culti-vated genetic background. Several biotechnological tools facilitate and increase the efficiency of introgressiomics. In this way, in vitro pollination, embryo rescue, and somatic hybridization can be useful for obtaining interspecific hybrids and backcrosses. Marker-assisted selection, using high-throughput genotyping techniques that survey the whole genome, is necessary for the development of collections of introgression lines (ILs) in which the whole genome of the donor CWR is represented among the different ILs. The use of molecular markers for developing high-density genetic maps coupled with phenotyping facilitates identifying genes and quan-titative trait loci (QTLs). Also, obtaining doubled haploids can be useful for fixation of the introgressed materials in the final steps. We exemplify the use of modern biotechnological tools in an egg-plant introgression breeding programme using an introgressiomics approach for adaptation to climate change.
https://doi.org/10.1016/j.jbiotec.2019.05.077
OP-APR12-A15
Puccinellia distans - A potential plant to reveal boron toxicity and salt tolerance mechanisms E.E. Hakki1,∗, A. Pandey1, M.K. Khan1, M. Hamurcu1, O. Celik2, S. Gezgin1, E. Atmaca1, M. Inanc1, T. Gumus2, O. Cakir3, C. Tarhan3, M. Sameeullah4
1Department of Soil Science and Plant Nutrition,
Faculty of Agriculture, Selcuk University, Konya 42250, Turkey
2Department of Molecular Biology and Genetics,
Istanbul Kultur University, Istanbul, Turkey
3Department of Molecular Biology and Genetics,
Faculty of Science, Istanbul University, Istanbul, Turkey
4Department of Biology, Faculty of Science and Arts,
Bolu Abant Izzet Baysal University, Bolu, Turkey
Boron toxicity, a widely observed abiotic stress, affects the yield and quality of the crops especially in arid and semi-arid agricul-tural regions. The biggest challenge in dealing with B toxicity is the genetic variation in plants. Puccinellia distans (Jacq.) Parl., a boron toxicity tolerant grass species, is found to endure extremely high Boron concentrations. We have conducted different experiments on Puccinellia species to reveal possible aspects of its B toxicity tol-erance. We estimated the physiological, biochemical and chemical changes in Puccinellia under Boron toxicity; outcomes of transcrip-tomics and expression analysis highlights its interlink with several biological processes. B toxicity is often associated with salt stress; in one of our experiments, characterization of B and Na efflux pro-teins directs towards B uptake and transport in plants roots that may have major role in maintaining plant homeostasis. We are also trying to determine the association between Puccinellia species and mycorrhizae that may have a significant role in soil reclama-tion. New crop development processes conducted by the breeders may further be empowered by novel B toxicity tolerant resources acquired from the knowledge gained from the detailed screening of P. distans species.
Acknowledgements
TUBITAK is gratefully acknowledged for funding (Project No 116Z343 & 116O866).
https://doi.org/10.1016/j.jbiotec.2019.05.078 OP-APR12-A21
New breakthrough CRISPR/Cas9 biotechnology of genome editing for creation of elite barley cultivars in Kazakhstan
O. Kershanskaya∗, D. Nelidova, G. Esenbaeva, G. Mukiyanova, A. Karabekova, S. Nelidov
Department of Cell Engineering, Institute Plant Biology and Bitechnology, Almaty, Kazakhstan
The technology, a genome-editing tool called CRISPR/Cas9, revo-lutionized the life sciences when it appeared on the market in 2013. CRISPR/Cas9 is RNA-regulated protection mechanism in bacteria and archaea, in which based new popular technology of gene edit-ing in human, animal and higher plants. CRISPR/Cas9 system is a simple, inexpensive and versatile tool for genome editing, resulting in a groundswell of research based on the technique which
