• Sonuç bulunamadı

Isolation of Chitinase Producing Bacillus Strains and Increasing of the Enzyme Production by In vitro Mutagenesis

N/A
N/A
Protected

Academic year: 2021

Share "Isolation of Chitinase Producing Bacillus Strains and Increasing of the Enzyme Production by In vitro Mutagenesis"

Copied!
4
0
0

Yükleniyor.... (view fulltext now)

Tam metin

(1)

27

th

INTERNATIONAL

SCIENTIFIC-EXPERT CONGRESS

OF AGRICULTURE AND

FOOD INDUSTRY

BOOK OF ABSTRACTS

26-28 September 2016, Bursa

TURKEY

(2)

27th International Scientific-Expert Congress of Agriculture and Food Industry, 26-28 September 2016, Bursa, Turkey

VII

CONTENTS

ORAL PRESENTATIONS

Enhancing Rice Growth, Physiological Characteristics and Yield by Symbiotic Fungus

Trichoderma asperellum SL2

Febri DONI*, Anizan ISAHAK, Che Radziah Che Mohd. ZAIN, Norela SULAIMAN, Wan Mohtar Wan YUSOFF

2

Changes in Peroxidase Isoenzyme and Protein Patterns in Oat Cultivars under Cold Stress

İmren KUTLU*, Ece TURHAN

3

A Novel Method for High-Frequency Transgenic Shoot Regeneration 1 via Agrobacterium

tumefaciens in Flax (Linum usitatissimum L.)

Ramazan BEYAZ*, E. Selcen DARÇIN, Murat AYCAN, Mustafa KAYAN, Mustafa YILDIZ

4

There is a Need for Pedagogical Approach to Agricultural Education

Ahmet ŞAHİN*, Atul KUMAR, Bahadır ALTUN

5

Food Safety Perception and Willing to Pay For Labelled Fish in Turkey: Case of Middle Mediterranean Region

Arzu SEÇER*, Esmeray Kuley BOĞA, Faruk EMEKSİZ

6

Determination of the Beef Consumption Habits and the Factors Affecting Beef Purchasing Decision of Households in the Province of Kırşehir

Başar ALTUNTAŞ*, Hasan Gökhan DOĞAN

7

The Impact of Turkish Agricultural Policy on Competitiveness of Oilseed Sunflower in Western Marmara Region, Turkey

Berna TÜRKEKUL*, Canan ABAY, Betül GÜRER, M. Necat ÖREN, Burhan ÖZALP

8

Cost Analysis of Farms That Produce Tomato in Soilless Greenhouses

Cihan SİPAHİOĞLU*, Tolga TİPİ

9

Impact of Agricultural Subsidies Policy on Turkey Dairy Farming by Using Probit Regression

Cuma AKBAY*, Abdulbaki BİLGİÇ, Emine İKİKAT TÜMER, Ahmed SAMİ

10

Adoption Characteristics of Livestock Farmers to Making Grass Silage in Ordu Province of Turkey

Çağatay YILDIRIM*, Hatice TÜRKTEN, İsmet BOZ

11

Perceptions of Risk and Uncertainty in Agricultural Production

Çiğdem ALP, Duygu AKTÜRK*

12

Analysis of Competitiveness of Turkey in the International Olive Oil Market

Derya İlkay ABDİKOĞLU*

13

Rural Tourism Potential of Osmaniye and Determination of Its’ Effect on Rural Development

Nermin BAHŞİ, Dilek BOSTAN BUDAK*

14

Comparative Analysis of Agricultural Credit System and Organization in Selected Countries

Erdoğan GÜNEŞ*, Hormoz MOVASSAGHI

(3)

27th International Scientific-Expert Congress of Agriculture and Food Industry, 26-28 September 2016, Bursa, Turkey

XI

An Evaluation of the Effects of Land Consolidation Projects Completed in Central Villages of Aydın Province

Safiye Pınar TUNALI*, Necdet DAĞDELEN, Talih GÜRBÜZ

60

The Effect of Drip and Furrow Irrigation on Yield Qualities Characteristics of Red Pepper in the Mediterranean Part of Turkey

Semih Metin SEZEN*, Asiye AKYILDIZ, Attila YAZAR, Yıldız DAŞGAN, Erdal AĞÇAM, Servet TEKİN

61

Isolation of Chitinase Producing Bacillus Strains and Increasing of the Enzyme Production by

In vitro Mutagenesis

Burcu DURMAZ, Bahri Devrim ÖZCAN*

62

Using Facilities of Propolis as a Feed Additive in Animal Nutrition

Banu YÜCEL*, Zümrüt AÇIKGÖZ

63

Behavioral Characteristics of Goats with Different Spatial Environment History in Structured Environment

Cemil TÖLÜ*,Volkan COŞKUN, Semra GÖKTÜRK,Türker SAVAŞ

64

The Relationship between Temperament and Maternal Behavior Score in Saanen X Hair Goat Crossbred Does

Serhat KARACA, Cihan ÇAKMAKÇI*, Muammer SARIBEY, Hasan ÜLKER, Gazel SER

65

Animal Existence and Production in Turkey

İbrahim AK*, Kadir Cem AKBAY, Arda SÖZCÜ, Ömer ŞENGÜL

66

Performance, Carcass Characteristics and Meat Quality in Fast and Slow Growing Genotypes Slaughtered at Certain Body Weight

Mikail BAYLAN*, Sibel CANOĞULLARI, Ayşen BULANCAK, Tugay AYAŞAN

67

Isolation and Identification of Vibrio anguillarum Strains in Rainbow Trouts (Oncorhynchus

mykiss Walbaum 1792) with Biolog Gen III in the Province of Kahramanmaraş

Elif DONAT, Mikail ÖZCAN*

68

Determination of in Vitro Digestibility of Soybean Meal and Canola Meal by Gas Production Technique

Sema YAMAN*,Mustafa BOĞA

69

Determining Correlation Analysis and Agro-Morphological Traits of Some Chickpea (Cicer

arietinum L.) Genotypes in Mediterranean Climate Conditions

Aybegün TON*, A. Emin ANLARSAL

70

The Effects of Different Row Spacings on Seed Yield of Some Common Vetch Varieties

Cafer Olcayto SABANCI*, Tamer YAVUZ, Hakan KIR,Sibel BAŞKÖY, Ali İhsan KARAYEL

71

Determination of Some Agronomical Characteristics of Local Pop Corn (Zea mays L. everta) Genotypes in Kahramanmaraş Conditions of Turkey

Gülay ZÜLKADİR*, Leyla İDİKUT, Mustafa ÇÖLKESEN

72

Effects of Salt and Drought Stresses on Germination and Seedling Growth of Sainfoin (Onobrychis viciifolia Scop.)

Gülcan DEMİROĞLU TOPÇU*, Şükrü Sezgi ÖZKAN

(4)

27th International Scientific-Expert Congress of Agriculture and Food Industry, 26-28 September 2016, Bursa, Turkey

62

Isolation of Chitinase Producing Bacillus Strains and Increasing of

the Enzyme Production by In vitro Mutagenesis

Burcu DURMAZ

1

, Bahri Devrim ÖZCAN

2*

1 Osmaniye Korkut Ata University, Graduate School of Natural and Applied Sciences, Department of Biology, 80000, Osmaniye, TURKEY

2 Osmaniye Korkut Ata University, Faculty of Arts and Sciences, Department of Biology, 80000, Osmaniye, TURKEY

*

Corresponding author: devrimozcan@osmaniye.edu.tr

In the present study, three chitinolytic Bacillus strains were isolated from the soil

samples containing shells of crustaceans collected from the coast sediments of Erzin

district of Hatay province. The isolates were entitled as Bacillus sp. DBK1, DBK2, and

DBK3, respectively. The optimum enzyme activities were observed at 40 °C for all

chitinases. On the other hand, optimum pH value for DBK1 chitinase was 7.0, whereas

6.0 for DBK2 and DBK3 chitinases. The specific activities of DBK1, DBK2, and DBK3

chitinases were 6.61, 13.17 and 8.65 U/mg at 40 °C, respectively. Maximum enzyme

productions of isolates were observed after 48, 36, and 60 hours later from inoculation

for DBK1, DBK2, and DBK3 isolates, respectively. Mutant varieties DBK1-M3 and

DBK1-M4 from DBK1, DBK2-M3, DBK2-M4 and DBK2-M5 from DBK2, and

DBK3-M3 and DBK3-M4 from DBK3 were obtained after ethidium bromide (EtBr)

treatment. DBK1-M3, DBK1-M4, DBK2-M3, DBK2-M4, DBK2-M5, DBK3-M3 and

DBK3-M4 have produced 146, 193, 192, 121, 136, 178 and 116% more chitinase

according to their own wild type strains, respectively. The activators for DBK1

chitinase were MnCl

2

, MgCl

2

, CaCl

2

, ZnCl

2

, and CuSO

4

, for DBK2 chitinase were

MnCl

2

, ZnCl

2

and KCl, and for DBK3 chitinase were MnCl

2

, CaCl

2

and ZnCl

2

. HgCl

2

strongly inhibited all wild type chitinases. These properties of the enzymes indicated

that they could be used as an enzyme supplement for aerobic stability of silage.

Keywords: Bacillus sp., Chitinase, Isolation, Characterization, Mutation, Silage

Referanslar

Benzer Belgeler

Diğer pek çok böcek grubunda olduğu gibi Taklaböcekleri, Cambaz böcekler veya Telkurtları gibi adlarla anılan Elateridae (Coleoptera) familyasına bağlı böcek türleri

09:20 - 09:40 Madencilik Sektöründe Jeneratör Seçimi, Kullanımı ve Önemi- İbrahim Fulin 09:40 - 10:00 Digital Transformation of Mining Enterprises- Dmitry Klebanov/ Ivan Perminov

Bu açıdan araştırmada tüketicilerin ürün ve hizmet üreten ve geniş kitlelere hitap edebilen çeşitli hazır giyim sitelerini (alibaba.com, boyner.com, collezione.com,

Tüketicilerin ürün/hizmet ile olan ilişkilerini analiz etmeye yardımcı olan bir yöntem olan Neden- Sonuç Zinciri Teorisi (Gutman, 1982; Reynolds, 1985), tüketicilerin satın

Bir önceki seçim de Doğu ve Güneydoğu Anadolu Bölgesi’ndeki toplamında sadece 2 milletvekili çıkarabilmiş olan CHP, 1957 Genel Seçimlerinde aday listelerinde

13:30-14:30 BIOLOGICS IN DERMATOLOGY SESSION Chairs: Serap Öztürkcan, Didem Didar Balcı. The history of monoclobal antibodies: Magic bullets Lawrence Parish Paradoxical

2 Bursa Teknik Üniversitesi Orman Fakültesi Peyzaj Mimarlığı Bölümü 16310, Bursa, TÜRKİYE Sorumlu Yazar: Salih PARLAK,

Halkbank müşterileri ile diğer 5 banka müşterileri karşılaştırıldığında Ziraat Bankası müşterilerinin marka değer algılarının Halkbank müşterilerinden