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Isolation of Feed Additive Xylanase Producing Bacillus Strain and Partial Characterization of the Enzyme

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27

th

INTERNATIONAL

SCIENTIFIC-EXPERT CONGRESS

OF AGRICULTURE AND

FOOD INDUSTRY

BOOK OF ABSTRACTS

26-28 September 2016, Bursa

TURKEY

(2)

27th International Scientific-Expert Congress of Agriculture and Food Industry, 26-28 September 2016, Bursa, Turkey

XV

POSTER PRESENTATIONS

Bioinformatic Analysis of LEA Proteins Associated with Salt Stress in Poacea

Fatih DEMİREL*, Serap DEMİREL

118

Determination of Genetic Diversity Among the ‘Patlıcan Fig’ (Ficus carica L.) Clones Using SSR and SRAP Markers

Hatice İKTEN*, Turan KARADENİZ, Yeliz YILMAZ, Tuba BAK, Cansu ŞİMŞEK

119

Effects of SNPS on the Secondary Protein Structure of Leptin Receptor Gene in the Long Term Selected Japanese Quails

Kemal KARABAĞ*, Sezai ALKAN, Taki KARSLI

120

A New Electrophoresis Technique to Separate Microsatellite Alleles’ “Qsep100 DNA Fragment Analyzer”

Koray KIRIKÇI*, Mehmet Akif ÇAM, Levent MERCAN

121

Potential of DNA Barcoding of Turkey's Endemic Plants Species and Conservation of Endemic Plants

Serap DEMİREL*, Fatih DEMİREL

122

In vitro culture Characters of Some Bread wheat Genotypes under Drought stress Condition

Sirwa Anwar QADIR, Mohammed Qader KHURSHEED, Fahrul Zaman HUYOP

123

Marker Assisted Selection for the Identification of Genotypes to Be Used in Pepper Breeding Program

Sümeyye ADALI*, Adem BARDAK, Ayhan AK

124

A Research on Dairy Cattle Production, Structure and Marketing Opportunities in Konya Province

Ahmad Jawid Abdul KHALIQ*, Cuma AKBAY

125

Teaching Agriculture by Using Production Tools

Bahadır ALTUN*, Ahmet ŞAHİN

126

Sustainability of Cotton Production in Turkey: An Analysis of Efficiency Using Data Envelopment Analysis

Betül GÜRER*, Berna TÜRKEKUL, Canan ABAY, M. Necat ÖREN, Burhan ÖZALP

127

Migration and Street Children

Kasım Eren TUNA, Dilek BOSTAN BUDAK*

128

Development of Agricultural Credit Market in Turkish Farming System

Erdoğan GÜNEŞ*, Hormoz MOVASSAGHI, Fahri ÜNSAL

129

The Evaluation of Agricultural Cooperative Movement in the World and Turkey

Murat YERCAN, Filiz KINIKLI*

130

Sustainable Agricultural Practices and Their Application in Environmentally Sensitive Areas

İsmet BOZ*

131

Analysis of Changes in Apple Production in Turkey

Kubilay UÇAR*,Sait ENGİNDENİZ,Todor MARKOVIĆ,Željko KOKOT

(3)

27th International Scientific-Expert Congress of Agriculture and Food Industry, 26-28 September 2016, Bursa, Turkey

XVII

Review and Evaluation of Legislation on Turkish Land Consolidation in Terms of Reallotment Principles

Musa AVCI*, Zafer Ali SERBES

150

Calibration of SPAD Leaf Chlorophyll Meter for Wheat

A. Mine YILDIZ TİLKİCİ*, Mustafa ÜNLÜ, Deniz Levent KOÇ, Müge ERKAN CAN

151

Drainage Water Quality Assessment Using the Irrigation Water Quality Index in Çarşamba Plain in Turkey

Alper GÜNGÖR, Hakan ARSLAN*

152

The Effects of Some Plant Nutrient Changes on Cotton Irrigated By Domestic Sourced Treated Wastewater

Mehmet GÜNDÜZ*, Nil KORKMAZ, Süleyman ŞEN, Şerafettin AŞIK, Perihan TARI AKAP

153

Occupational Accident Status of Farmers’ Family Members and Agricultural Workers: A Study in Adana

Özkan GÜĞERCİN*, Nafi BAYTORUN, Deniz Levent KOÇ, Yusuf Yiğit ÖZEN

154

Using Moringa oleifera in Poultry Nutrition

Ahmet Önder ÜSTÜNDAĞ*, Mürsel ÖZDOĞAN

155

The Effects of Pre-heating Period on the Hatchability and Chick Development of Ostrich (Struthio camelus domesticus) Eggs

Arda SÖZCÜ*,Aydın İPEK

156

Comparison of Total Numbers of Individual in Commercial Bombus terrestris Colonies Produced by Two Different Breeders

Ayhan GÖSTERİT*, Abdullah Nuri ÖZSOY, Sedat AKTAN, Aşkın GALİÇ, Fehmi GÜREL

157

Production Time and Number of Sexuals in Bombus terrestris Colonies Which Fed with Diets Including Soya Flour

Şehriban SAĞLAM, Ayhan GÖSTERİT*

158

Isolation of Feed Additive Xylanase Producing Bacillus Strain and Partial Characterization of the Enzyme

Zübeyde BAŞARAN, Bahri Devrim ÖZCAN*

159

Use of Silkworm Pupae Meal as A Protein Source in Broiler and Laying Hen Diets

Zümrüt AÇIKGÖZ*, Banu YÜCEL

160

Growth and Health of Lambs in a Hoop Barn

Başak PEHLİVAN, Türker SAVAŞ*

161

Blood Parameters of Laying Hens Infested with Dermanyssus gallinae

Esra ÜNBAŞ, Nazif YAZGAN, Baver Coşkun*, Coşkun KONYALI, Serdar KAMANLI, Türker

SAVAŞ

162

Behavior of Lambs and Goat Kids which Raised in Wood Slatted and Straw Floors

Cem ATALAY, Mert MANDACI, Türker SAVAŞ, Cemil TÖLÜ*

163

Effect of Dermanyssus gallinae on Egg Quality in White Layers

Nazif YAZGAN, Esra ÜNBAŞ, Coşkun KONYALI, Baver Coşkun*, Serdar KAMANLI, Türker

SAVAŞ

(4)

27th International Scientific-Expert Congress of Agriculture and Food Industry, 26-28 September 2016, Bursa, Turkey

159

Isolation of Feed Additive Xylanase Producing Bacillus Strain and

Partial Characterization of the Enzyme

Zübeyde BAŞARAN

1

, Bahri Devrim ÖZCAN

2*

1 Osmaniye Korkut Ata University, Graduate School of Natural and Applied Sciences, Department of Biology,

80000, Osmaniye, TURKEY

2 Osmaniye Korkut Ata University, Faculty of Arts and Sciences, Department of Biology, 80000, Osmaniye,

TURKEY

* Corresponding author: devrimozcan@osmaniye.edu.tr

The aerobic and xylanolytic bacterium Bacillus sp. XbO was isolated from soil sample.

The results of 16S rDNA sequence comparisons indicated that Bacillus sp. XbO was

closely related to Bacillus pumilus and Bacillus safensis with 98% levels of sequence

homology. The optimum temperature and pH values of the enzyme were found to 60 ºC

and 6.0 respectively. The residual activities of the enzyme after pre-incubation at 70 ºC

and 11.0 pH value for 30 min, were 86 and 79% respectively. Maximum enzyme

production was observed after an incubation period of 24 hours, at 37 ºC. The enzyme

activity was increased 173, 167, 144, 141, 133, 126, and 112% in the presence of

MnCl

2

, CoCl

2

, ZnCl

2

, MgCl

2

, CaCl

2

, EDTA, and FeCl

3

(5 mM) respectively. On the

other hand, xylanase activity was strongly inhibited in the presence of HgCl

2

. The

specific activities of crude and purified enzymes were 27.6 and 113.8 U/mg proteins,

respectively. Electrophoretic analysis showed the presence of single band having

molecular weight of about 81 kDa. These properties of the enzyme indicated that it

could be used as an enzyme supplement for animal feed.

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