İnsan adipoz dokusundan mezenkimal kök hücrelerinin eksplant kültür tekniğiyle i̇zolasyonu

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ll & Tissue Biology Research

Turkish Histology and Embryology Society

www.ctbiol.com

Volume 5 / 2016 Supplement

Special Issue

Includes abstracts of the

13th National Histology and Embryology Congress with International

Participation

April 30- May 3, 2016

Ilica Hotel

Cell and Tissue Biology Research is an official journal of the Turkish Histology and Embryology Association. It is an online journal publishing research articles after full peer review. All articles are published, without barriers to access, immediately upon acceptance. One volume is published every year. Each volume consists of 4 numbers published quarterly online.

Aims and Scope

Cell and Tissue Biology Research is a peer-reviewed journal that publishes original research on all aspects of anatomy, histology, cell biology and fine structure of tissues and organs on light and electron microscopical level, neuroanatomy, and morphological techniques, as well as developmental biology, focusing on morphogenesis—the study of the emergence of form during embryogenesis, mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, and genetic levels. Published manuscript styles include original research articles, review articles, technical notes, case reports, short communications, and letters to the editor. Novel features include: full peer review, high quality of reproduction, rapid publication, no page charges, wide readership, and fast track submission.

Indexing and archiving

Cell and Tissue Biology Research aims to be indexed in all major national and international databases in the neer future.

Copyright

All rights reserved. Apart from any relaxations permitted under national copyright laws, no part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means without the prior written permission of the copyright owners. Permission is not required to copy summaries of papers or of articles on the condition that a full reference to the source is given. Multiple copying of the contents without permission is always illegal.

Contact Information

Queries about content, submissions, or the review process should be directed to the Turkish Histology and Embryology Association.

If you have proposals or feedback related to this journal or this field of science, please do not hesitate to contact the Managing Editor Dr.A. Cevik Tufan, E-mail: actufan@pau.edu.tr.

Readership

Mainly consists of (but not limited to) histologists, microscopists, developmental biologists, embryologists, molecular and cellular biologists, biochemists, geneticists, neurobiologists, anatomists, pathologists, physiologists.

Instructıons to Authors

Cell and Tissue Biology Research publishes original research articles, review articles, technical notes, case reports, short communications, and letters to the editor within the scope of the journal. The content of the Cell and Tissue Biology Research is determined by the Editors.

The manuscript which is submitted to the journal must not contain previously published material or material under consideration for publication elsewhere, except for a preliminary report in abstract format. Accepted manuscripts become the property of Cell and Tissue Biology and may not be republished.

The decision on acceptance of manuscripts for publication in Cell and Tissue Biology Research will be made on the basis of a peer review system.

Responsibilities of the Authors

By submitting a manuscript for publication, each author acknowledges having made a substantial contribution in the conception and design of the study, the analysis and interpretation of the results, and the writing of the paper, and has approved the final submitted version of the paper. Each author thus also acknowledges responsibility for the integrity of the manuscript, assures the originality of the manuscript, and guarantees that duplicate or redundant publications or submissions have not occurred. The Editors reserve the right to request the original data obtained in the investigation. Authors are responsible for all statements made in the text.

The text document must be saved as Word or RTF format. Tables must be included in the text document. Figures must be saved in the formats and at the resolution indicated below (illustrations section).

The manuscript should be typed double-spaced throughout on one side of A4 paper with at least a 2.5 cm margin on all sides. Do not divide words at the end of lines. Pages should be numbered consecutively in Arabic numerals, beginning with the title page.

Prepare a cover letter and a copyright transfer form signed by all authors.

Organize the manuscript as follows: title page, abstract (including key words at the end), introduction, material and methods, results, discussion (including the conclusions), acknowledgments, references, figure legends, and tables.

Keep acronyms and abbreviations to a minimum. When an abbreviation is used, define it at first mention and follow with the abbreviation in parentheses.

Categories of Submission Review Articles

Cell and Tissue Biology Research publishes review articles on the basis of invitation by the editor(s). However, author(s) is free to suggest topics and manuscripts for publication in the journal as a rewiev article as well. The author(s) is absolutely free to design the paper. There is no limitation in the page count in this category. References, figures, and legends follow the guidelines described below under ‘Original Articles.’ The Abstract section is needed.

Original Articles

Title Page. The following information should appear: title of article; authors’ name, and last name; affiliations with complite addresses. Identify the corresponding author and provide full mailing address, phone and fax numbers, and e-mail address. Also provide a short running title (no more than 40 spaces).

Abstract. The abstract is limited to 400 words, and should describe the essential aspects of the investigation. In the first sentence state the background; in the second sentence state your specific purpose or hypothesis; in the third, fourth and fifth sentences summarize methods, results and conclusion. No references should be cited. For indexing purposes, a small number of "key words" (no more than 5) must be supplied.

Introduction. Include brief background information on what has been done in the past in this area and the importance of your investigation. End with a statement of the purpose or hypothesis of the study.

Material and Methods. This section may be divided into subsections if it facilitates reading the paper. The research design, subjects, material used, and statistical methods should be included. Do not mix results and discussion into this section. Do not include manufacturer’s names unless the specific product is important to the procedures performed, in which case the city and state or country of the manufacturer should also be given. Indicate that informed consent has been obtained from patients who participated in clinical investigations. In animal experimentation, acknowledge that ethical guidelines were followed. When appropriate, indicate that approval was obtained from the institution’s review board.

Results. This section may be divided into subsections if it facilitates reading the paper. All results based on methods must be included. If tables and graphic material will ease the understanding of the results, include them. Cite figures to illustrate the findings of the study.

Discussion. Start with limited background information and then discuss the results of the investigation in light of what has been published in the past, the limitations of your study, and potential directions for future research. In appropriate place, cite figures and graphs. Following this information, summarize the major findings of the study and their clinical usefulness. This paragraph should address the hypothesis or purpose stated earlier in the paper.

Acknowledgments. Acknowledgments should appear on a separate page. Tis section also has to include the grant information (if any) of the investigators.

References. Section must be double spaced and begin on a separate page. References to the literature should be cited in the text by the name of the author(s) followed by the year of publication. In cases in which there are

example (Smith, 1981a). When two or more references are included in the same bracket, they must be quoted in the chronological order; example (Smith, 1980; Bell et al., 1984). All references must be cited in the text, and all authors should be listed in references. The reference list should be in alphabetical order.

References to articles in periodical publications must include: Names and initials of all authors, year of publication, complete title of paper, name of journal (abbreviated in accordance with Index Medicus), number of volume, and first and last page numbers. Example: Morita T., Suzuki Y. and Churg J. (1973). Structure and development of the glomerular crescent. Am. J. Pathol. 72, 349-368.

References to books must include: Name and initials of authors, year of publication, full title, edition, editor, publisher, place of publication and page numbers. Example: Powell D. and Skrabanek P. (1981). Substance P. In: Gut Hormones. 2nd ed. Bloom S.R. and Polak J.M. (eds). Churchill Livingstone. Edimburgh. pp 396-401. References to URL (Web Page) must include: Name and initials of URL owners, full title of the URL, address, and accession date in pharantheses. Example: Stern M. Radial nerve entrapment. http://www.emedicine.com/orthoped/topic549.htm (accessed Dec 2005).

Tables. Each table should be given on a separate page. Each table has a short, descriptive title. Tables are numbered in the order cited in the text. Abbreviations are defined as footnotes at the bottom of each table. Tables should not duplicate data given in the text or figures.

Figures and Legends. The complete sets of original figures must be submitted. Subjects’ names must not appear on the figures. Labels should contrast well with the background. Images should be uniform in size and magnification. Illustrations should be free of all identifying information relative to the subject and institution. Line drawings should be professional in quality. Written permission for use of all previously published illustrations must be included with submission, and the source should be referenced in the legends. Written permission from any person recognizable in a photo is required. Legends must be double spaced, and figures are numbered in the order cited in the text. Submit color prints only if color is essential in understanding the material presented. Label all pertinent findings.

Illustrations should be labelled with the figure number and author's name in soft pencil on the back identifying the top edge. Photographs should be glossy bromide prints of good contrast and well matched, preferably not mounted on card. Photographs should not exceed 17.8 x 22.2 cm. The Editor reserves the right to reduce or enlarge the illustrations. Colour photographs will be allowable only in special circumstances. Line diagrams should be drawn with black ink on tracing paper or white card or supplied as glossy prints. Illustrations should be submitted protected by resistant cardboard. Apply figure numbers to the lower left-hand corner of each photograph; dry transfer lettering (such as letraset) may be used. Digital images are welcome. They must be submitted on CDROM (CD-r or CD-RW) only. We cannot use other types of disk. Images should be TIFF file format, preferentially, although other formats could be useful. Black and white figures must be at gray scale. Color figures should be preferentially in CMYK, but RGB is also allowed. Line art files must have a 500dpi resolution, while other images must have a 300dpi resolution. Supplying digital images is not a substitute for the press set of figures.

Technical Notes

While the journal encourages the submission of full-length original articles, it will consider the publication of technical notes describing the characteristics of new instruments

of methodological improvements. In addition to a title page (formatted as described above), include a summary (250 word) describing the essence of the report, an introduction (two or three sentences of background information), a description of the technique, and a discussion highlighting the educational value of the technique. References should be limited (no more than 10 preferred) to only those that give essential background material. References, figures, and legends follow the guidelines described above in ‘Original Articles.’

Short Communications

While the journal encourages the submission of full-length original articles, it will consider the publication of short communications ensuring rapid publication of new/preliminary results of unusually educational and medically important. Whole manuscript should be maximum of 3 pages, containing maximum of 1 figure, and 1 table. In addition to a title page (formatted as described above), include a summary (250 word) describing the essence of the report, an introduction (two or three sentences of background information); materails and methods, results and a discussion highlighting the educational value of the case. References should be limited (no more than 10 preferred) to only those that give essential background material. References, figures, and legends follow the guidelines described above in ‘Original Articles.’

page (formatted as described above), include a summary (250 word) describing the essence of the report, an introduction (two or three sentences of background information); the case report (written in the past tense), and a discussion highlighting the educational value of the case. References should be limited (no more than 10 preferred) to only those that give essential background material. References, figures, and legends follow the guidelines described above in ‘Original Articles.’

Letters to the Editor

Letters to the Editor may be used to describe in an extremely brief manner either an observation of interest to our readers, an opinion relative to the Cell and Tissue Biology Research, or constructive

observations or criticisms of published material. Letters should be no more than two pages and should be submitted with a brief title. A maximum of four references may be included. Letters are published at the discretion of the journal and are subject to editing.

Cell and Tissue Biology Research Editorial Board (for this issue) Editors-in-Chief: Petek KORKUSUZ, M.D. Ph.D. Ayşegül UYSAL, Ph.D. Gülperi ÖKTEM, M.D. Ph.D. Managing Editors: A. Çevik TUFAN Çiğdem ELMAS

Alev Gürol BAYRAKTAROĞLU Özhan EYİGÖR

Çiler ÇELİK ÖZENCİ Ayten TÜRKKANI Özgür ÇINAR

Prof. Dr. Gülçin Abban Mete Prof. Dr. Hüseyin Aslan Prof. Dr. Sevim Aydın Prof. Dr. Ayhan Bilir Prof. Dr. Belgin Can Prof. Dr. İlhami Çelik Prof. Dr. Suzan Dağlıoğlu Prof. Dr. Necdet Demir Prof. Dr. Güven Erbil Prof. Dr. Feriha Ercan Prof. Dr. Esra Erdemli Prof. Dr. Ender Erdoğan Prof. Dr. Hatice Erdost Prof. Dr. Oya Evirgen

Prof. Dr. Berrin Gençer Tarakçı Prof. Dr. Aydın Girgin

Prof. Dr. Alpaslan Gökçimen Prof. Dr. Mehmet Gül

Prof. Dr. Ranan Gülhan Aktaş Prof. Dr. Aysel Güven Bağla Prof. Dr. Hasan Herken Prof. Dr. Sevinç İnan Prof. Dr. Tülay İrez Prof. Dr. Zeynep Kahveci Prof. Dr. Sabiha Serpil Kalkan Prof. Dr. Turan Karaca

Prof. Dr. Erdal Karaöz Prof. Dr. M. Aydın Ketani Prof. Dr. Ahmet Koç Prof. Dr. Emel Koptagel Prof. Dr. Emin Türkay Korgun Prof. Dr. Meral Koyutürk Prof. Dr. Şadiye Kum Prof. Dr. Aysel Kükner Prof. Dr. Sevda Müftüoğlu Prof. Dr. Yusuf Nergiz Prof. Dr. Ersan Odacı Prof. Dr. Asuman Özen Prof. Dr. Nesrin Özfiliz Prof. Dr. Meltem Özgüner Prof. Dr. Candan Özoğul Prof. Dr. Sait Polat

Prof. Dr. Bizden Sabuncuoğlu Prof. Dr. Yavuz Tekelioğlu Prof. Dr. İbrahim Tuğlu

Yrd. Doç. Dr. Hayrunnisa Yeşil

Prof. Dr. Serpil Ünver Saraydın Prof. Dr. Birkan Yakan

Prof. Dr. Melda Yardımoğlu Yılmaz Prof. Dr. Altuğ Yavaşoğlu

Prof. Dr. Engin Yenilmez Prof. Dr. Selma Yılmazer Prof. Dr. Serap Arbak Prof. Dr. Utku Ateş

Prof. Dr. Banu Coşkun Yılmaz Prof. Dr. Figen Kaymaz Prof. Dr. Özgül Tap

Prof. Dr. Sema Girgin Timurkaan Prof. Dr. H. Seda Vatansever Doç. Dr. Nuray Acar

Doç. Dr. Cevat Aktaş Doç. Dr. Selen Bahçeci Doç. Dr. Ebru Ballı Doç. Dr. Sevil Çaylı Doç. Dr. Kemal Ergin Doç. Dr. Süheyla Gonca Doç. Dr. Emine Elif Güzel Doç. Dr. Yıldırım Kalkan Doç. Dr. Sibel Serin Kılıçoğlu Doç. Dr. Cem Korkmaz Doç. Dr. Meltem Kuruş Doç. Dr. Emin Oğuzhan Oğuz Doç. Dr. Hakan Sağsöz Doç. Dr. Leyla Satı Doç. Dr. Nejdet Şimşek Doç. Dr. Gamze Tanrıöver Doç. Dr. Şehime Gülsün Temel Doç. Dr. Yeter Topçu Tarladaçalışır Doç. Dr. Selçuk Tunik

Doç. Dr. Elgin Türköz Uluer Doç. Dr. Yiğit Uyanıkgil Doç. Dr. Yusufhan Yazır Yrd. Doç. Dr. Seyit Ali Bingöl Yrd. Doç. Dr. Alev Cumbul Yrd. Doç. Dr. Gökhan Cüce Yrd. Doç. Dr. Hakan Darıcı Yrd. Doç. Dr. Mustafa Erboğa Yrd. Doç. Dr. Pınar Naile Gürgör Yrd. Doç. Dr. İlknur Keskin Yrd. Doç. Dr. Fatma Bahar Sunay

Hürriyet Caddesi, No:10/6 Dikmen-ANKARA-TURKEY

Journal title: Cell and Tissue Biology Research.

Manuscript title: ... ... ... Full names of all authors (in order to appear on manuscript): ... ... Name, address etc. of corresponding author: ... ... ... ID Number: ... Telephone: ... E-mail: ... Mobile phone: ...

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d) the manuscript has not been published and is not being submitted or considered for publication elsewhere;

e) the text, illustrations, and any other materials included in the manuscript do not infringe upon any existing copyright or other rights of anyone.

Notwithstanding the above, the Contributor(s) or, if applicable the Contributor(s) Employer, retain(s) all proprietary rights other than copyright, such as

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b) to use, free of charge, all parts of this article for the author(s) future works in books, lectures, classroom teaching or oral presentations; c) the right to reproduce the article for their own purposes provided the copies are not offered for sale.

However, reproduction, posting, transmission or other distribution or use of the article or any material contained therein, in any medium as permitted hereunder, requires a citation to the Journal and appropriate credit to THEA as publisher, suitable in form and content as follows: Title of article, author(s), journal title and volume/issue, Copyright© year.

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I/We indemnify THEA and the Editors of the Journals, and hold them harmless from any loss, expense or damage occasioned by a claim or suit by a third party for copyright infringement, or any suit arising out of any breach of the foregoing warranties as a result of publication of my/our article. I/We also warrant that the article contains no libelous or unlawful statements and does not contain material or instructions that might cause harm or injury.

This copyright form must be signed by all authors. Separate copies of the form (completed in full) may be submitted by authors located at different institutions; however, all signatures must be original.

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Sayın Meslektaşlarımız,

XIII. Ulusal Histoloji ve Embriyoloji Kongresi, Ege Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı’nın ev sahipliği ve Türk Histoloji ve Embriyoloji Derneği’nin işbirliğinde 30 Nisan-3 Mayıs 2016 tarihinde Çeşme Ilıca Otelinde gerçekleştirilmektedir. Alanlarında uzman 14 uluslararası ve 17 ulusal davetli konuşmacının yer aldığı bu yıl ki kongremiz, ‘’her hücre bir hücreden doğar’’ anlamınına gelen, ‘’omnis cellula e cellula’’ mottosuyla gerçekleştirilmektedir.

Türk Histoloji ve Embriyoloji Derneği, kurulduğu 1990 yılından bugüne, üye sayısını her geçen gün arttırarak güçlenmiş; alanını temsil eden tek meslek derneği olarak; üyelerinin eğitim, hizmet ve araştırma alanlarındaki etkinliklerini ulusal ve uluslararası platformlarda desteklemeyi amaç edinmiştir. İki yılda bir gerçekleştirilen ulusal kongrelerimiz bu etkinliklerin en önemli bölümünü oluşturmaktadır. Derneğimizin kuruluşunun 25. Yıl dönümünü de kutladığımız bu yıl ki

kongremizde, histoloji ve embriyoloji araştırma alanının güncel konuları olan kök hücre ve rejeneratif tıp, üreme ve gamet biyolojisi, tümör biyolojisi ve hücresel tedaviler gibi başlıklar yer almaktadır. Kongre kapsamında 8 konferans oturumu, Türkiye Bilimler Akademisi’nin desteklediği bir kök hücre oturumu, bir minipanel, 10 çalışma grubu oturumu, 5 eğitim ve eşgüdüm oturumu ile 3 sözlü ve 3 poster sunusu oturumu gerçekleştirilmektedir.

Her ulusal kongremizde olduğu gibi, bu yıl da davetli konuşmalar, sözlü ve poster sunularının Türkçe ve İngilizce özetlerini elektronik olarak burada yayımlıyoruz. Dergimizde paylaştığınız bilgi ve deneyimlerinizin histoloji ve embriyoloji alanına çok önemli katkılar sağlayacağına inanıyoruz. Hem bilimsel, hem de sosyal anlamda değerli katılımlarınızla zenginleştirdiğiniz Uluslararası katılımlı XIII. Ulusal Histoloji ve Embriyoloji Kongresi’nde sizleri İzmir’in ve Ege’nin güzelliği ve sıcaklığında buluşturmanın mutluluğuyla hepinize en iyi dileklerimizi sunarız.

Saygı ve sevgilerimizle.

Kongre Eş Başkanları Kongre Genel Sekreteri

Prof. Dr. Ayşegül UYSAL Ege Üniversitesi

Tıp Fakültesi

Histoloji ve Embriyoloji Anabilim Dalı Başkanı

Prof. Dr. Petek KORKUSUZ Türk Histoloji ve Embriyoloji

Derneği 13. Dönem Yönetim Kurulu Başkanı

Prof. Dr. Gülperi ÖKTEM Ege Üniversitesi

Tıp Fakültesi Histoloji ve Embriyoloji

2

Onur Kurulu / Honorary Board

Ege Üniversitesi Rektörü / Rector of Ege University Prof. Dr. Candeğer YILMAZ

Ege Üniversitesi Rektör Yardımcısı / Vice-chancellor of Ege University Prof. Dr. Yeşim KİRAZLI

Dekan / Deputy Dean of Ege University Faculty of Medicine Prof. Dr. Cemil GÜRGÜN

Kongre Eş-Başkanları / Co- Chairs

Prof. Dr. Ayşegül UYSAL, EÜTF Histoloji ve Embriyoloji Anabilim Dalı Başkanı / Ege University Faculty of Medicine, Head of Department of Histology & Embryology

Prof. Dr. Petek KORKUSUZ, THED Yönetim Kurulu Başkanı / President of Turkish Society of Histology & Embryology

Kongre Genel Sekreteri / Congress Secretariat

Prof. Dr. Gülperi ÖKTEM, EÜTF Histoloji ve Embriyoloji Anabilim Dalı / Ege University Faculty of Medicine, Department of Histology & Embryology

Kongre Düzenleme Kurulu / Congress Organizing Committee

Türk Histoloji ve Embriyoloji Derneği / Turkish Histology & Embryology Society Prof. Dr. Petek KORKUSUZ (Başkan)

Prof. Dr. A. Çevik TUFAN (Başkan Yardımcısı) Prof. Dr. Çiğdem ELMAS (Genel Sekreter) Doç. Dr. Alev Gürol BAYRAKTAROĞLU (Sayman) Prof. Dr. Özhan EYİGÖR

Prof. Dr. Gülperi ÖKTEM (Kongre Genel Sekreteri) Prof. Dr. Çiler ÇELİK ÖZENCİ

Doç. Dr. Ayten TÜRKKANI Doç. Dr. Özgür ÇINAR

Yerel Düzenleme Kurulu / Local Organizing Committee

Ege Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı / Ege University Faculty of Medicine, Department of Histology & Embryology

Prof. Dr. Ayşegül UYSAL (Başkan) Prof. Dr. Meral BAKA

Prof. Dr. Hüseyin AKTUĞ Prof. Dr. Gülperi ÖKTEM Prof. Dr. Altuğ YAVAŞOĞLU Doç. Dr. Yiğit UYANIKGİL Doç. Dr. Özlem YILMAZ Uzm. Dr. Türker ÇAVUŞOĞLU Uzm. Dr. Fatih OLTULU Ar. Gör. Eda AÇIKGÖZ Ar. Gör. Gürkan YİĞİTTÜRK Dr. Kenan DEMİR

Dr. Aylin GÖKHAN Dr. Duygu KOCATÜRK Ar. Gör. Kubilay Doğan KILIÇ Ar. Gör. Çevik GÜREL

Ar. Gör. Berrin ÖZDİL Dok. Öğr. Dr. Gurur GARİP Yük. Lis. Öğr. Aylin BUHUR Yük. Lis. Öğr. Yasemin ADALI Yük. Lis. Öğr. Ceren KUŞÇU

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Prof. Dr. Gülçin Abban Mete Prof. Dr. Hüseyin Aslan Prof. Dr. Sevim Aydın Prof. Dr. Ayhan Bilir Prof. Dr. Belgin Can Prof. Dr. İlhami Çelik Prof. Dr. Suzan Dağlıoğlu Prof. Dr. Necdet Demir Prof. Dr. Güven Erbil Prof. Dr. Feriha Ercan Prof. Dr. Esra Erdemli Prof. Dr. Ender Erdoğan Prof. Dr. Hatice Erdost Prof. Dr. Oya Evirgen

Prof. Dr. Berrin Gençer Tarakçı Prof. Dr. Aydın Girgin

Prof. Dr. Alpaslan Gökçimen Prof. Dr. Mehmet Gül

Prof. Dr. Ranan Gülhan Aktaş Prof. Dr. Aysel Güven Bağla Prof. Dr. Hasan Herken Prof. Dr. Sevinç İnan Prof. Dr. Tülay İrez Prof. Dr. Zeynep Kahveci Prof. Dr. Sabiha Serpil Kalkan Prof. Dr. Turan Karaca Prof. Dr. Erdal Karaöz Prof. Dr. M. Aydın Ketani Prof. Dr. Ahmet Koç Prof. Dr. Emel Koptagel Prof. Dr. Emin Türkay Korgun Prof. Dr. Meral Koyutürk Prof. Dr. Şadiye Kum Prof. Dr. Aysel Kükner Prof. Dr. Sevda Müftüoğlu Prof. Dr. Yusuf Nergiz Prof. Dr. Ersan Odacı Prof. Dr. Asuman Özen Prof. Dr. Nesrin Özfiliz Prof. Dr. Meltem Özgüner Prof. Dr. Candan Özoğul Prof. Dr. Sait Polat

Prof. Dr. Bizden Sabuncuoğlu Prof. Dr. Yavuz Tekelioğlu Prof. Dr. İbrahim Tuğlu

Prof. Dr. Serpil Ünver Saraydın Prof. Dr. Birkan Yakan

Prof. Dr. Melda Yardımoğlu Yılmaz Prof. Dr. Altuğ Yavaşoğlu

Prof. Dr. Engin Yenilmez Prof. Dr. Selma Yılmazer Prof. Dr. Serap Arbak Prof. Dr. Utku Ateş

Prof. Dr. Banu Coşkun Yılmaz Prof. Dr. Figen Kaymaz Prof. Dr. Özgül Tap

Prof. Dr. Sema Girgin Timurkaan Prof. Dr. H. Seda Vatansever Doç. Dr. Nuray Acar

Doç. Dr. Cevat Aktaş Doç. Dr. Selen Bahçeci Doç. Dr. Ebru Ballı Doç. Dr. Sevil Çaylı Doç. Dr. Kemal Ergin Doç. Dr. Süheyla Gonca Doç. Dr. Emine Elif Güzel Doç. Dr. Yıldırım Kalkan Doç. Dr. Sibel Serin Kılıçoğlu Doç. Dr. Cem Korkmaz Doç. Dr. Meltem Kuruş Doç. Dr. Emin Oğuzhan Oğuz Doç. Dr. Hakan Sağsöz Doç. Dr. Leyla Satı Doç. Dr. Nejdet Şimşek Doç. Dr. Gamze Tanrıöver Doç. Dr. Şehime Gülsün Temel Doç. Dr. Yeter Topçu Tarladaçalışır Doç. Dr. Selçuk Tunik

Doç. Dr. Elgin Türköz Uluer Doç. Dr. Yiğit Uyanıkgil Doç. Dr. Yusufhan Yazır Yrd. Doç. Dr. Seyit Ali Bingöl Yrd. Doç. Dr. Alev Cumbul Yrd. Doç. Dr. Gökhan Cüce Yrd. Doç. Dr. Hakan Darıcı Yrd. Doç. Dr. Mustafa Erboğa Yrd. Doç. Dr. Pınar Naile Gürgör Yrd. Doç. Dr. İlknur Keskin Yrd. Doç. Dr. Fatma Bahar Sunay Yrd. Doç. Dr. Hayrunnisa Yeşil

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PROGRAM - PROGRAM SCHEDULE

30 Nisan 2016, Cumartesi - Saturday, 30 April 2016 ANA SALON - MAIN HALL

10:00-17:30 Kayıt - Registration

12:30-13:30 Açılış töreni - Opening ceremony 13:30-14:10 Açılış konuşmaları

Welcome remarks Ayşegül Uysal Ege Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı Başkanı

Head of Histology and Embryology Department Ege University Faculty of Medicine

Petek Korkusuz THED Başkanı

President of Turkish Histology and Embryology Society

Cemil Gürgün

Ege Üniversitesi Tıp Fakültesi Dekanı

Dean of Ege University Faculty of Medicine

Yeşim Kirazlı

Ege Üniversitesi Rektör Yardımcısı

Vice President of Ege University

14:10-14:40 Açılış konferansı - Keynote lecture Oturum Başkanları: Petek Korkusuz,

A. Çevik Tufan Developmental and regenerative skeletogenesis: Principles,

strategies, promises, and challenges Rocky S. Tuan K1 University of Pittsburgh School of Medicine

14:45-15:45 Açılış oturumu: Yeniden programlama

Opening lectures: Reprograming Oturum Başkanları: Ayşegül Uysal, Serap Arbak 14:45-15:15 Uniting major constituents of the genome: A novel function

of the Piwi-piRNA pathway in the germline Haifan Lin K2 Yale Stem Cell Center

15:15-15:45 Regulation of somatic cell reprogramming and its application In-Hyun Park K3

Yale Stem Cell Center

15:45-16:00 Kahve arası - Coffee break

16:00-17:30 Sözlü bildiri oturumu 1 - Oral presentations 1

Üreme ve gamet biyolojisi - Reproductive and gamete

biology

Oturum Başkanları: Suzan Dağlıoğlu, Şahin Sırmalı, Candan Özoğul, İsmail Seçkin 16:00-16:10 Prostat Kanser Kök Hücresi ve Embriyo Ortak Kültürlerinde

Yeniden Programlanmanın Epiteliyal Mezenşimal Değişim Yönüyle Araştırılması

Investigating reprogramming in co-cultures of prostate cancer stem cell and embrio in terms of epithelial mesenchymal transition

Fatih Oltulu

Ege Üniversitesi Tıp Fakültesi

16:10-16:20 Fare Oositi ve Preimplantasyon Dönemi Embriyolarında mTERT Telomeraz Katalitik Alt ünitesinin c-Abl Protein Tirozin Kinaz Tarafından Düzenlenmesi

Regulation of The mTERT Telomerase Catalytic Subunit by The c-Abl Protein Tyrosine Kinase during Oocyte and Preimplantation Mouse Embryos

Aylin Yaba Uçar

Yeditepe Üniversitesi Tıp Fakültesi

5

16:20-16:30 Endokannabinoid agonistleri, endometriyotik hücrelerde hücre çoğalma indeksini düşürmektedir

Endocannabinoid agonists decrease cell proliferation indexes on endometriotic cells

Elif Bilgiç

Hacettepe Üniversitesi Tıp Fakültesi

16:30-16:40 Embriyonik Poli(A)-Bağlanma Proteinine Özgü Poliklonal Antikor Üretimi

Producing a Polyclonal Antibody Specific to Embryonic Poly(A)-binding Protein

Fatma Uysal

Akdeniz Üniversitesi Tıp Fakültesi

16:40-16:50 Testiküler İskemi Reperfüzyon Hasarında Ubikütin Proteazom Yolağının Rolü

Role of the Ubiquitin proteaosome pathway proteins in testicular ischaemia reperfusion injury

Seda Ocaklı

Gaziosmanpaşa Üniversitesi Tıp Fakültesi

16:50-17:00 Tetrasiklin-indüklenebilir Ctcfl transgenik farelerde H19 DMD metilasyon paterni

H19 DMD methylation pattern in tetracycline-inducible Ctcfl transgenic mice

Leyla Satı

Akdeniz Üniversitesi Tıp Fakültesi

17:00-17:10 FoxO transkripsiyon faktörlerinin peri-implantasyon sürecindeki rolleri: Endometriyal reseptivitenin olası yeni belirteci The roles of FoxO transcription factors in

endometrium during peri-implantation period: Possible new marker of endometrial receptivity

Dileyra Adıgüzel Akdeniz Üniversitesi Tıp Fakültesi

17:10-17:20 Frajil X Primer Ovaryum Yetmezligi Fare Modelinde Folikul Atrezisinin ve Oosit Kalitesinin Degisimi

Follicle Atresia and Oocyte Quality are Altered in a Mouse Model of Fragile X Primary Ovarian Insufficiency

Bahar Uslu

Yale Tıp Fakültesi, Kadin Dogum ve Ureme Bilimleri Departmani 17:20-17:30 In Vitro Elde Edilmiş Dört-Hücre Evresindeki Sığır

Embriyolarında Kontrollü Yavaş Dondurma veya Vitrifikasyon Sonrası Ultra-Strüktürel Değişiklikler

Ultra-Structural Alterations in In Vitro Produced Four-Cell Bovine Embryos Following Controlled Slow Freezing or Vitrification

Türker Çavuşoğlu

Ege Üniversitesi Tıp Fakültesi

17:30-18:30 Poster bildirileri - Poster presentations

Sistemler, Kök hücre, Kanser

Oturum Başkanları: Murat Tosun, Yiğit Uyanıkgil, Özgür Çınar, Pergin Atilla, Cem Korkmaz, Alev Gürol Bayraktaroğlu 19:30 Açılış etkinliği - Opening reception

01 Mayıs 2016, Pazar - Sunday, 01 May 2016 ANA SALON - MAIN HALL

08:30-17:30 Kayıt - Registration

08.30-09:40 Konferans: Üreme biyolojisi 1

Conference: Reproductive biology 1 Oturum Başkanları: Alp Can, Sevda Müftüoğlu, Mukaddes Eşrefoğlu

08:30-08:55 Progesterone signaling and pregnancy complications: Implications of cellular and molecular mechanisms for therapeutic management of prematurity

Charles J. Lockwood K4

University of South Florida Health Morsani College of Medicine

08:55-09:20 Cellular and molecular regulators of progestin-induced abnormal uterine bleeding; from bench discoveries to novel clinical treatment options

Ümit Kayışlı K5

University of South Florida College Of Medicine

6

09:20-09:40 Zaman meselesi: İmplantasyon ve sirkadyan saat

A matter of time: Implantation and circadian clock Çiler Çelik Özenci K6 Akdeniz Üniversitesi Tıp Fakültesi

Akdeniz University Faculty of Medicine

09:40-09:55 Ara - Break

09:55-11:10 Konferans: Rejeneratif tıp

Conference: Regenerative medicine Oturum Başkanları: Selma Yılmazer, Özhan Eyigör, İbrahim Tuğlu

09:55-10:20 Adult stem cells and biomimetic matrices for tissue

engineering and modeling: Repair, restore, and re-create Rocky S. Tuan K7 University of Pittsburgh School of Medicine

10:20-10:45 Claudin 6 and epidermal differentiation: Use of animal

models Kursad Turksen K8 Editor in Chief, Stem Cell Reviews and Reports

10:45-11:10 Macrophage-osteoprogenitor cell crosstalk in osteogenesis Stuart B. Goodman K9

Stanford University School of Medicine

11:10-11:20 Kahve arası - Coffee break

11:20-12:35 Türkiye Bilimler Akademisi -TÜBA oturumu: Türkiye'de kök hücre çalışmaları Turkish Academy of Sciences session:

Stem cell studies in Turkey

Oturum başkanı: Gülperi Öktem

11:20-11:35 Ege Üniversitesi Kök Hücre Anabilim Dalı lisansüstü eğitim deneyimi

Ege University Department of Stem Cell graduate education experience

Gülperi Öktem K10

Ege Üniversitesi Tıp Fakültesi

Ege University Faculty of Medicine

11:35-11:55 Türkiye'de klinik kök hücre uygulamaları

Implementation of clinical stem cell therapies in Turkey Taner Demirer K11 Ankara Üniversitesi Tıp Fakültesi

Ankara University Faculty of Medicine

11:55-12:15 İskemik kardiyomiyopatide kök hücre yaklaşımları

Stem Cell Therapy Approaches to Ischemic Cardiomyopathy Alp Can K12 Ankara Üniversitesi Tıp Fakültesi

Ankara University Faculty of Medicine

12:15-12:35 Nöromuskuler dejeneratif hastalıklarda kök hücre uygulamaları: Laboratuvardan kliniğe

Stem Cell Applications on neuro-muscular degenerative disorders: Bench to bedside

Erdal Karaöz K13

Liv Hospital Rejeneratif Tıp ve Kök Hücre Üretim Merkezi Hastanesi

Liv Hospital Regenerative Medicine Stem Cell Production Center

12:35-13:30 Öğle yemeği - Lunch

14:00-15:30 Konferans: Hücresel tedaviler ve klinik yaklaşımlar

Conference: Cell therapies and approaching clinics Oturum Başkanları: Zeynep Kahveci, Esra Erdemli, Hüseyin Aktuğ, Sevim Aydın

14:00-14:25 Osteonecrosis: Etiology to stem cell therapy Stuart B. Goodman K14

Stanford University School of Medicine

14:25-14:50 Hematopoietic specification of embryonic and induced pluripotent stem cells and their potential use in cell-based therapies

Annelise Bennaceur Griscelli K15

University Paris Sud XI School of Medicine

14:50-15:15 Modelling cancer stem cells using iPSC Ali Turhan K16

University Paris Sud XI School of Medicine

15:15-15:35 p300 ve CBP kromatin düzenleyicilerinin yeniden programlamadaki rolleri

A chromatin modifier-based chemical screen identifies a role for p300 and CBP in reprogramming

Tamer Önder K17

Koç Üniversitesi Tıp Fakültesi

Koc University Faculty of Medicine

7

15:50-16:30 Minipanel: Makale yazımı

Minipanel: Writing a manuscript Oturum Başkanları: Oya Evirgen, Ahmet Nacar 15:50-16:10 What journals are looking for: One editor's perspective Kursad Turksen K18

Editor in Chief, Stem Cell Reviews and Reports

16:10-16:30 İyi bir sözel sunum için basit kurallar

Simple rules for making good oral presentation Şahin Sırmalı K19 Uludağ Üniversitesi Tıp Fakültesi

Uludag Universitry Faculty of Medicine

16:30-17:50 Sözlü bildiri oturumu 2 - Oral presentations 2

Hücre doku hasarı - Cell and tissue injury Oturum Başkanları: Yusuf Nergiz, Sait Polat, Meral Baka, Engin Yenilmez

16:30-16:40 Koroner arter hastalığında epikardiyal yağ dokusunda makrofaj infiltrasyonu ve polarizasyonu üzerine netrin-1'in etkisi

Netrin-1 modulates macrophage infiltration and polarization in human epicardial adipose tissue in coronary artery disease

Kadri Murat Gürses Hacettepe Üniversitesi Tıp Fakültesi

16:40-16:50 Neonatal farede kardiyak rejenerasyon mekanizmalarinin araştırılması

Analysis of underlying molecular mechanisms of neonatal cardiac regeneration

Doğacan Yücel Yeditepe Üniversitesi Mühendislik Fakültesi 16:50-17:00 Gebelik Sürecinde Diyabetik Sıçan Plasentalarında mTOR

Sinyal Yolağının Anjiyogenez Mekanizmasına Etkisi

Effect of mTOR Signalling Pathway on Angiogenesis Mechanisms in Diabetic Rat Placenta During Pregnancy

Aslı Özmen

Akdeniz Üniversitesi, Tıp Fakültesi

17:00-17:10 Nazal polipozis’ de apoptozla ilişkili yolaklar: MAPK/JNK ve PI3K/mTOR Yolaklarının önemi

Apoptosis related pathways in the nasal polyposis: Importance of MAPK/JNK and PI3K/mTOR Pathways

Fatma Şimşek

İzmir Katip Çelebi Üniversitesi

17:10-17:20 Sjögren Sendromu Tanılı Hastalarda, Tükrük Bezinde Mast Hücreleri ve Notch Sinyalizasyonu İlişkisinin Araştırılması

The Relation Between Mast Cells and Notch Signalization In The Salivary Glands of Patients Diagnosed With Sjogren’s Syndrome

Sema Avcı Akdeniz Üniversitesi, Tıp Fakültesi

17:20-17:30 Deneysel olarak oluşturulmuş hipotiroid, hipertiroid ve ötroid modellerinde miyokardiyal yağ asidi metabolizması ile inflamasyon arasındaki ilişkinin incelenmesi

Investigate the relationship between myocardial fatty acid metabolism and inflammation at experimentally induced hypothyroidism, hyperthyroidism, and euthyroid models

Ezgi Nuriye Bektur Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi

17:30-17:40 Eritropoietin'nin Deneysel Parkinson Sıçan Modelinde Nöroprotektif Etkisi

The neuroprotective effect of erythropoietin on experimental Parkinson model in rats

Kubilay Doğan Kılıç

Ege Üniversitesi Tıp Fakültesi

17:40-17:50 Nesfatin Nöronlarında Glutamat Reseptörlerinin Varlığının Agonist Etkileri ve Reseptör Ekspresyonu Düzeyinde Araştırılması

Assessment of the Presence of Glutamate Receptors in Nesfatin Neurons through the Agonist Effects and Receptor Expression

Duygu Gök Yurtseven Uludağ Üniversitesi, Sağlık Bilimleri Enstitüsü

8

Üreme ve gamet, Güncel biyomedikal yaklaşımlar, Embriyoloji

Oturum Başkanları: Feral Öztürk, Nesrin Özfiliz, Güven Erbil, Gamze Tanrıöver, Ayten Türkkanı, Barış Baykal

19:00 Akşam yemeği - Dinner

02 Mayıs 2016, Pazartesi - Monday, 02 May 2016 ANA SALON - MAIN HALL

08:30-17:30 Kayıt - Registration

08.30-10:00 Konferans: Tümör biyolojisi 1

Conference: Tumour biology 1 Oturum Başkanları: Ayhan Bilir, Rüçhan Uslu, Sevinç İnan, Seda Vatansever

08:30-08:55 Role of TGF-superfamily during different stages of prostate

cancer Shafiq A. Khan K20 Clark Atlanta University NIH/RCMI Program

08:55-09:20 Microenvironmental regulation of exosomal-ncRNAs in

cancer Gabriel Lopez-Berestein K21 MD Anderson Cancer Center

09:20-09:45 Development of novel targeted therapies for cancer:

Targeting EF2-kinase Bülent Özpolat K22 MD Anderson Cancer Center

09:45-10:00 Regulation of anoikis in ovarian cancer Burcu Aslan K23

MD Anderson Cancer Center

10:00-10:15 Kahve arası - Coffee break

10:15-12:35 Sözlü bildiri oturumu 3 - Oral presentations 3 Kök hücreler, hücresel tedavi ve rejeneratif tıp

Stem cell, cell therapies and regenerative medicine

Oturum Başkanları: İsmail Üstünel, Emel Koptagel, Birkan Yakan, Aysel Kükner, Bünyami Ünal

10:15-10:25 Fare fibroblast, fare akciğer kanseri ve fare embriyonik kök hücrelerinde flavopiridol ve geldanamisinin terapötik etkilerinin ve hücre döngüsü bileşenleri, apopitozis, hücre iskeleti ilişkili moleküllerin karşılaştırılması

Comparison of cell cycle components, apoptosis and cytoskeleton related molecules and therapeutic effects of flavopiridol and geldanamycin in mouse fibroblast, mouse lung cancer and mouse embryonic stem cells

Hüseyin Aktuğ

Ege Üniversitesi Tıp Fakültesi

10:25-10:35 Genoma entegre olmayan RNA virüsü aracılığı ile sağlıklı ve osteopetrotik insan mezenkimal kök hücrelerinin feeder-free şartlarda yeniden programlanması

Reprogramming of donor and osteopetrotic human

mesenchymal stem cells with non-integrating RNA virus on feeder-free conditions

İnci Cevher

Hacettepe Üniversitesi Tıp Fakültesi

10:35-10:45 Deneysel hasarlı sıçan testislerinde yağ doku kökenli kök hücre etkilerinin oksidatif stres ile ilişkisinin histolojik ve biyokimyasal parametreler ile karşılaştırılması

Effects adipocyte derived stem cell in the experimentally damaged rat testis with evaluation of histologic and biochemiacal parameters together relation of oxidative stress

Dila Hatun Sal

Celal Bayar Üniversitesi Tıp Fakültesi

Celal Bayar University Faculty of Medicine

10:45-10:55 Hücresel dormansinin küçük moleküllerle engellenmesi hematopoetik kök hücrelerinin ex vivo çoğaltılmasını sağlamaktadırTargeting cellular quiescence by small

molecules enables ex vivo hematopoietic stem cell expansion

Fatih Kocabaş Yeditepe Üniversitesi Mühendislik Fakültesi

9

10:55-11:05 Fare vitiligo modelinde in vitro çoğaltılan dermis kökenli hücrelerin tedaviye yönelik kullanımının ışık ve elektron mikroskobu düzeyinde araştırılması

Investigation of in vitro cultured dermis derived cells for treatmental purpose in a mouse vitiligo model using light and electron microscopy

Aslı Erdoğan

İstanbul Üniversitesi, İstanbul Tıp Fakültesi

11:05-11:15 Mezenkimal Kök Hücreler Hematopoetik Kök Hücrelerin Mobilizasyonunu Endokannabinoidler Aracılığı İle Düzenlemektedir

Mesenchymal Stem Cells Regulate The Hematopoietic Stem Cell Mobilization Through The Endocannabinoids

Sevil Köse

Hacettepe Üniversitesi Tıp Fakültesi

11:15-11:25 Kordon kanı kök hücrelerinin diyabetik ayak yaralarında iyileşmeyi sağlayan faktörler üzerine etkisinin

immünohistokimyasal yöntemle gösterilmesi

The Indication of the Influence of Cordon Blood Stem Cells on the Factors that Provide Healing on Diabetic Foot Scars Using Immunohistochemical Method

Nazlı Çil

Pamukkale Üniversitesi Tıp Fakültesi

11:25-11:35 Dişoluşturmak için yeni bir umut: öncül hücrelerle harmanlanmış hidroksi apatit grefti

A new hope for a tooth generation: hydroxy apatite scaffold combined with progenitor cells

Pakize Neslihan Taşlı

Yeditepe Üniversitesi Mühendislik Fakültesi

11:35-11:45 Erişkin fare ovaryumu kök hücreleri ve yeniden oogenez olasılığı

Adult mouse ovarian stem cells and possibility of neo-oogenesis

Yashar Esmaeilian Ankara Üniversitesi, Biyoteknoloji Enstitüsü 11:45-11:55 Ubikitin Aktifleştirici E1 Gen Ailesinin Hematopoetik Kök

Hücre Dormansisi ve Kardiyomiyosit Tutuklanmasındaki Rolü

Ubiquitin Activating E1 Family at the Crossroads between Stem Cells Quiescence and Cardiomyocyte Cell Cycle Arrest

Merve Aksöz

Yeditepe Üniversitesi Mühendislik Fakültesi

11:55-12:05 Çocukluk çağı akut miyeloid lösemilerinde kemik iliğinden köken alan mezenkimal stromal hücrelerin biyolojik ve immünolojik özellikleri

The biological and immunological properties of

mesenchymal stromal cells derived from bone marrow in childhood acute myeloid leukemia

İlkay Pişkin

Yıldırım Beyazıt Üniversitesi Tıp Fakültesi

12:05-12:15 Kemik iliği ve plasenta dokusu Mezenkimal Kök Hücreler'inin immünomodülatuar ve farklılaşma kapasitelerinin karşılaştırılması

Comparison of immunomodulatory and differentiation capacities of Mesenchymal Stem Cells derived from bone marrow and placental tissue

Aysun Sarıkaya

Hacettepe Üniversitesi Sağlık Bilimleri Enstitüsü

12:15-12:25 Dermis kökenli hücrelerin diyabetik yara modeli iyileşmesi üzerine etkisinin ışık ve elektron mikroskobu düzeyinde araştırılması

Light and electron microscopic investigation of the effects of dermis derived cells of diabetic wound healing model

Hasan Serdar Mutlu

İstanbul Üniversitesi, İstanbul Tıp Fakültesi

12:25-12:35 Yeni bir multi duvarlı karbon nanotüp tabanlı yapı

iskelesinin insan meme kanseri hücre hattı (mcf-7) üzerine biyouyumluğunun incelenmesi

A novel multi walled carbon nanotube based scaffold and its biocompatibility in human breast adenocarcinoma cells

Pınar Kılıçaslan Sönmez Celal Bayar Üniversitesi Tıp Fakültesi

10

14:00-14:30 Uydu sempozyumu: Cytation 5 mikroskopi görüntüleme ve ölçüm sistemleri

Satellite symposium: Cytation 5 microscopy imaging and

measurement systems Orhan Korkmaz, Biotek

14:30-14:40 Ara - Break

14:40-15:45 Konferans: Tümör biyolojisi 2 Conference: Tumour biology

2 Oturum Başkanları: İlkin Çavuşoğlu, Alper Bağrıyanık, Altuğ Yavaşoğlu, Çiğdem Elmas 14:40-15:00 Lityum klorür ve antineoplastik ajanların farklı tümör hücre

kültürleri üzerine etkilerinin ototfajik ve ultrastrüktürel olarak incelenmesi

The autophagic activity of LiCl and antineoplastic agents on different tumor cell lines using electron microscopy

Ayhan Bilir K24

Zirve Üniversitesi Tıp Fakültesi

Zirve University Faculty of Medicine

15:00-15:15 Tedaviye direnç mekanizması olarak fenotipik plastisite

Phenotypic plastisity as a mechanism of therapy resistance Zeynep Yüce K25 Dokuz Eylül Üniversitesi Tıp Fakültesi

Dokuz Eylul University Faculty of Medicine

15:15-15:30 Klinik pratikte kök hücre nakli: Laboratuvardan hastaya

Stem cell transplantation in clinical practice: From the laboratory to the patient

Güray Saydam K26

Ege Üniversitesi Tıp Fakültesi

Ege University Faculty of Medicine

15:30-15:45 Laboratuvar - pilot ölçeklerde monoklonal antikor üretimine çeşitli örnekler ve sorunlar

Some laboratory & pilot scale monoclonal antibody production techniques and milestones

İsmet Deliloğlu Gürhan K27 Ege Üniversitesi Mühendislik Fakültesi

Ege University Faculty of Engineering

15:45-16:00 Kahve arası - Coffee break 16:00-17:30 THED genel kurul toplantısı

Turkish Histology and Embryology Society general assembly

17:30-18:30 Poster bildirileri - Poster presentations

Hücre ve doku hasarı, Histoloji ve embriyoloji’de bilimsel yaklaşımlar ve eğitim

Oturum Başkanları: Aydan Özgörgülü, Gülnur Kızılay Özfidan, Narin Liman, Dilara Zeybek, Özlem Yılmaz, Meltem Kuruş

20:00 Gala yemeği - Gala dinner

03 Mayıs 2016, Salı - Tuesday, 03 May 2016 ANA SALON - MAIN HALL

08:50-10:10 Konferans: Üreme biyolojisi 2, teknikler

Conference: Reproductive biology 2, techniques Oturum Başkanları: Nejdet Demir, Semiha Ersoy, Saim Özdamar, Artay Yağcı, Hatice Erdost

08:50-09:10 Transcriptomics analysis and bioinformatics approaches for

histology and embryology studies Ümit Kayışlı K28 University of South Florida College Of Medicine

09:10-09:25 IVF sikluslarında elde edilen germinal vezikül (GV) evresindeki oositler hasta yararına kullanılabilir mi?

Can germinal vesicle (gv) stage oocytes acquired in ivf cycles be used to the benefit of the patient?

Barış Baykal K29

Gülhane Askeri Tıp Akademisi

Gulhane Military Medical Academy School of Medicine

09:25-09:40 A new rapid non-arbitrary histomorphometric method of

quantifying ovarian follicle atresia in the mouse Bahar Uslu K30 Yale Üniversitesi Tıp Fakültesi

Yale University School of Medicine

11

Genetic tests in prenatal screening Özgür Çoğulu K31 Ege Üniversitesi Tıp Fakültesi

Ege University Faculty of Medicine

09:55-10:10 Tüp bebek ünitesinin kuruluşu ve işleyişindeki deneyimlerimiz

Our experiences during set up and operating IVF unit

Tahsin Murad Aktan K32 Necmettin Erbakan Üni. Tıp Fakültesi

Necmettin Erbakan University Faculty of Medicine

10:10-10:25 Kahve arası - Coffee break

10:25-10:55 Konferans: Kök hücre uygulamaları

Conference: Stem cell applications Oturum Başkanları: Berrin Zık, Işıl Tekmen 10:25-10:40 Veteriner hekimlikte kök hücre kullanımı

Stem cell theraphy in veterinary medicine Asuman Özen K33 Ankara Üniversitesi Veteriner Fakültesi

Ankara Üniversity Faculty of Veterinary Medicine

10:40-10:55 PRP'nin klinik kullanımındaki deneyimlerimiz

Our Clinical Usage Experiences For PRP Selçuk Duman K34 Necmettin Erbakan Üni. Tıp Fakültesi

Necmettin Erbakan University Faculty of Medicine

12

Davetli Konuşma ve Konferanslar Invited Lectures and Conferences (K01-K34)

13

Rocky S. Tuan - University of Pittsburgh School of Medicine

Developmental and regenerative skeletogenesis: Principles, strategies, promises, and challenges Rocky S. Tuan

Center for Cellular and Molecular Engineering Department of Orthopaedic Surgery

University of Pittsburgh School of Medicine

Formation of the skeletal system gives the organism form and structure, and is a key event of embryonic development. Skeletogenesis is a culmination of patterning, cellular condensation and differentiation, extracellular matrix production, morphogenesis of structural elements, and

mechanical activation. Scientific investigations in the last many decades have provided a large body of information on the cellular origin and activities and molecular signaling events that are responsible skeletal development, a truly 3-dimensional morphogenetic event of development. Diseases, traumatic injuries, and aging are known to cause structural damages to the adult

skeleton. Regenerative medicine is being touted as a promising approach to address these medical needs, utilizing the principles of tissue development, involving progenitor cells, enabling matrix scaffolds, and appropriate biological signals. Focusing on skeletal tissues in various in vitro and in vivo experimental models, this lecture will address the concepts guiding this approach, the

technical strategies being employed, and the promises and the challenges ahead to realize the potential of regenerative medicine.

14

Haifan Lin - Yale Stem Cell Center

Uniting major constituents of the genome: A novel function of the Piwi-piRNA pathway in the germline

Toshiaki Watanabe, Ee-chun Cheng, and Mei Zhong, and Haifan Lin Yale Stem Cell Center and Department of Cell Biology,

Yale University School of Medicine, New Haven, Connecticut 06519, USA

The eukaryotic genome has vast intergenic regions containing transposons, pseudogenes, repetitive sequences, and noncoding genes that produce numerous long non-coding RNAs

(lncRNAs) and PIWI-interacting RNAs (piRNAs). Yet the functions of the intergenic regions remain largely unknown. In mammals, a unique set of piRNAs, pachytene piRNAs, is abundantly expressed in the germline in late spermatocytes and early spermatids. Recently, we showed that piRNAs derived from transposons and pseudogenes mediate the degradation of a large number of mRNAs and lncRNAs in mouse late spermatocytes. In particular, they have a large impact on the lncRNA transcriptome, as a quarter of lncRNAs expressed in late spermatocytes are upregulated in mice deficient in piRNA pathway. Furthermore, our genomic and in vivo functional analyses revealed that retrotransposon sequences are frequently found in the 3' UTR of mRNAs that are targeted by piRNAs for degradation. Similarly, the degradation of spermatogenic cell-specific lncRNAs by piRNAs is mediated by retrotransposon sequences. Moreover, we have shown that pseudogenes regulate mRNA stability via the piRNA pathway. The degradation of mRNAs and lncRNAs by piRNAs requires MIWI and, at least in part, depends on its slicer activity. Together, these findings reveal a highly complex and global RNA regulatory network through which transposons and pseudogenes regulate target mRNA and lncRNA stability via the piRNA pathway to promote

15

In-Hyun Park - Yale Stem Cell Center

Regulation of somatic cell reprogramming and its application

My research foci include investigating the epigenetic regulation of cell fate to understand the human somatic cell reprogramming and to develop cellular therapeutics for human

neurodevelopmental disorders. Recently, my lab has worked on dissecting the cellular and molecular progresses of human somatic cell reprogramming. An analysis of transcriptome

transition from somatic to pluripotent cells demonstrated the bimodal change in signaling pathways and identified novel primate specific reprogramming factors. In addition, I have led the research on defining the function of MeCP2 on Rett syndrome (RTT) and developing therapeutics for RTT. We have established in vitro RTT model using human iPSCs and found that metabolic genes are abnormally regulated in human RTT neurons.

16

Charles J. Lockwood - University of South Florida Health Morsani College of Medicine Progesterone signaling and pregnancy complications: Implications of cellular and molecular mechanisms for therapeutic management of prematurity

Prof. Charles J. Lockwood, MD. Department of Obstetrics and Gynecology, School of Medicine, University of South Florida, Tampa, FL.

Human term labor is triggered by sequential molecular modifications that involve activation of inflammation and inhibition of progesterone-progesterone receptor (P4-PR) function in the

reproductive tract. Infection, placental abruption, maternal/fetal stress, genetic and environmental factors cause spontaneous preterm birth (PTB). Worldwide over 15 million PTBs occur each year. Only in the USA, more than 12% of live births occur prematurely, constituting a major public health burden due to attendant perinatal mortality and morbidity costing $26 billion/year. Decidual Inflammation is an essential feature of both chorioamnionitis (CAM) and abruption-induced PTBs. In all viviparous species, inhibition of P4 production and/or function elicits labor (i.e., activates decidual inflammation, remodels the cervix, promotes fetal membrane rupture and increases myometrial contractility). P4 signaling in target cells is achieved by binding to two P4 receptor (PR) isoforms: PR-A (92 kDa) and PR-B (116 kDa), which belong to a ligand-activated nuclear

transcription factor superfamily. Both PR isoforms bind P4 and progestins such as MPA and R5020 with equal affinity. Ligand binding induces PR dimerization, phosphorylation and binding to cis-acting response elements on DNA to modulate activity of target gene promoters. In several cell lines, PR-B is a transcriptional activator, whereas PR-A is a transcriptional repressor of

P4-responsive promoters. Unlike most mammals in which parturition is initiated by declining maternal plasma P4 levels, this systemic decline does not occur during primate and guinea pig (GP)

parturition. In these mammals, elevated P4 levels are sustained until after delivery of the placenta suggesting that a physiologic block of P4 signaling in target cells elicits functional P4 withdrawal. Treatment of women with PR antagonists (e.g. RU486) induces labor at any stage of pregnancy indicating that PR is responsible for functional P4 withdrawal. This talk will involve discussions on potential mechanisms that contribute to functional P4 withdrawal include decreased PR gene transcription, increased ubiquitin-mediated PR degradation, PR phosphorylation changes, PR isoform switching (PR-A vs. PR-B), changes in PR co-regulators, and/or indirect antagonism by other transcription factors. We will also discuss the inhibitory effects of proinflammatory cytokines and immunophilin FKBP51 on PR expression and function in decidual cells and how these inhibitions of PR expression and function mediate functional P4 withdrawal at the maternal-fetal interface triggering inflammation and stress induced PTB.

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Ümit Kayışlı - University of South Florida College Of Medicine

Cellular and molecular regulators of progestin-induced abnormal uterine bleeding; from bench discoveries to novel clinical treatment options

Assoc. Prof. Umit A Kayisli, PhD Department of Obstetrics and Gynecology, School of Medicine, University of South Florida, Tampa, FL.

The US has an extremely high rate of unintended pregnancies. Non-adherence to contraceptives accounts for nearly half of these unintended pregnancies, over a million pregnancies annually. Unintended pregnancies result in excess preterm birth, low birth weight and are significant contributors for perinatal and maternal morbidity and mortality. The total public costs for unintended pregnancies is around $21 billion/year. Abnormal uterine bleeding (AUB) is irregular uterine bleeding that occurs in the absence of recognizable pelvic pathology, general medical disease, or pregnancy. Long term progestin use is one of the main causes of AUB. Albeit progestin only long acting reversible contraceptives (pLARCs) are safely used worldwide, they are most often discontinued due to AUB. This talk will include our results that uncovered basic mechanisms underlying such AUB. Progestins markedly reduces endometrial perfusions resulting in hypoxia (HX) and reperfusion-induced ROS generation. HX/ROS may cause AUB directly by damaging endometrial microvessels and indirectly by inducing aberrant angiogenesis. The latter reflects HX/ROS effects on: human endometrial stromal cells (HESCs) to decrease expression of the vascular stabilizing protein, angiopoietin-1 (Ang-1) and increase levels of VEGF, the primary initiator of angiogenesis; and endometrial endothelium to increase expression of Ang-2, a vessel branching and elongation factor. Unlike menstrual bleeding originating from spiral arterioles in response to P4 withdrawal, pLARCs-associated AUB occurs intermittently and focally from irregularly distributed superficial, abnormally enlarged, fragile microvessels. This talk will further discuss molecular and cellular mechanisms generating these damages in microvessels. Progestin-induced microvascular damage contributes to excess thrombin generation by increasing delivery of circulating factor VII to HESC-derived TF. While thrombin prevents bleeding by activating platelets and generating fibrin, it promotes focal hemorrhage by binding to HEEC-expressed protease activated receptors (PARs) to increases endothelial permeability and aberrant angiogenesis and inflammation by increasing VEGF and IL-8 expression and degrades endometrial matrix by inducing HESC-derived MMP-1 and MMP-3. We will discuss our recent discoveries on endothelial cells and vascular smooth muscle cells (VSMCs). Our microarray and proteomics analyses reveal that progestin-treated HESCs secrete inducers of endothelial apoptosis. Moreover, progestin-treated superficial endometria and pLARC-treated guinea pigs display reduced numbers of VSMCs, which prevents envelopment of new vessels and contributes to the hyperdilated, thin-walled fragile microvessels. Ingenuity pathway analysis identifies progestins-reduced CCL2 levels are responsible for this suppression of VSMC proliferation. The concerted actions of HX-induced HESC-mediated HEEC apoptosis and progestin-inhibited VSMC proliferation via CCL2 suppression provides several novel therapeutic approaches to prevent AUB.

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Çiler Çelik Özenci - Akdeniz Üniversitesi Tıp Fakültesi/Akdeniz University Faculty of Medicine

Zaman meselesi: İmplantasyon ve sirkadyan saat

A matter of time: Implantation and circadian clock

İmplantasyon penceresi; pre-implantif gelişimini tamamlamış olan blastosistin reseptif uterus ile karşılaştığı kısıtla bir zaman dilimini ifade eder. Embriyo implantasyonu blastosist ve endometriyum arasında, karmaşık mekanizmalar tarafından düzenlenen, karşılıklı bir ilişki gerektirir. Endometriyal epitel hücreleri, endometriyal bez hücreleri ve endometriyal stroma hücrelerinin uterus

reseptivitesine katkıları hakkında bilgiler henüz yeterli değildir. Sadece implante olan embriyo değil, bu hücre kompartımanları da birbirleriyle özgün gen ekspresyonları ile karşılıklı olarak konuşurlar. Son yıllarda, sirkadyan ritim düzenleyicilerinin üreme ve fertilite ile ilişkili olduğu ve zaman sistemlerinde meydana gelen bozuklukların insanlarda ve farelerde üreme kapasitesini olumsuz etkileyebildiği bildirilmektedir. Sirkadyan saat ile ilişkili genlerin tekli delesyonu veya mutasyonu sadece sirkadyan ritmin bozulmasına değil üreme fonksiyonunun da etkilenmesine sebep

olmaktadır. CLOCK proteininin bir paraloğu olan (bir tek genomda gen ikilenmesi ile oluşmuş ve işlevleri farklılaşmış, benzer gen) ve sirkadyan saati kontrol eden NPAS2, hücrelerde CLOCK eksikliğinde meydana gelen düzensizlikleri kompanse edebilir. NPAS2 ekspresyonunu, normal gebelik sürecine ek olarak yalancı gebelik ve yapay desidualizasyon oluşturulmuş fare modellerinde araştırdık. Ayrıca, fertilizasyon sonrasında 6 saatlik ileri faz zaman dilimine gidiş modellenerek sirkadyan saatleri bozulan farelerde implantasyon ve sonraki gebelik dönemi değerlendirildi. Konuşmanın içeriğinde fare peri-implantasyon sürecinde NPAS2’nun varlığı ve olası rolü hakkındaki bulgularımızı tartışılacaktır. Gece vardiyasında çalışanların ve uluslararası seyahat edenlerin artışıyla ve bu süreçlerin insan sirkadyan ritmini bozduğu bilgisine dayanarak, fertilitede sirkadyan ritmin olası rolünü değerlendirmek üreme biyolojisi alanında çalışanlar açısından güncel bir

konudur.

The window of implantation is a limited time span when blastocyst competency is superimposed on the receptive state of the uterus. Embryo implantation requires a reciprocal interaction between the blastocyst and endometrium and is associated with complex regulatory mechanisms. Contribution of endometrial epithelial, glandular, and stromal cell types to uterine receptivity is poorly

understood. Not only the implanting embryo is in crosstalk with these cellular compartments but also these compartments are in crosstalk between each other with specific gene expression and mouse models with compartment-specific gene deletion became informative. In recent years, circadian rhythm regulators have been linked to the regulation of reproduction and fertility such that disruptions to timing systems can adversely influence reproductive capacity. Deletion or mutation of single genes results not only in disrupted circadian rhythmicity, but also compromised reproductive function. NPAS2, a paralogue of CLOCK protein, can compensate for loss of CLOCK in cells. We have studied NPAS2 expression during peri-implantation period and in various mouse models including pseudo-pregnancy, and artificial decidualization. We evaluated implantation and onwards in phase-advance group that was subjected to 6-hour advances of the light cycle. This talk will focus on our findings regarding the presence and possible regulation of NPAS2 during peri-implantation period in mice. Given the increased incidence of shiftwork and international travel which disrupt circadian rhythmicity in humans it is important for reproductive medicine scientists to consider the role of circadian rhythms in fertility.

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Rocky S. Tuan - University of Pittsburgh School of Medicine

Adult stem cells and biomimetic matrices for tissue engineering and modeling: Repair, restore, and re-create

Rocky S. Tuan

Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA

Degenerative joint diseases, the most prevalent cause of physical disabilities, affect up to 15% of the population, particularly the elderly. In osteoarthritis, the low, intrinsic reparative capacity of cartilage is a clinical challenge to effective treatment. Current treatments, such as

anti-inflammatory drugs, are only able to provide short-term pain relief. Total joint arthroplasty remains the only effective procedure, but is ultimately limited by the finite life expectancy of the implant. Tissue engineering and regenerative medicine, an emerging scientific discipline encompassing translational application of cells, scaffolds, and biological signals, is a potentially promising approach to repair damaged/diseased tissues to restore joint function and mobility. Adult

mesenchymal stem cells (MSCs), from tissue sources such as bone marrow, adipose, and skeletal muscle, exhibit multi-lineage mesenchymal differentiation potential, including chondrogenesis, and are considered a promising candidate cell type for cartilage repair. A critical component to

successful cell-based cartilage tissue engineering is a biocompatible biomaterial cell-carrier scaffold that ideally also enhances proliferation and differentiation of the seeded cells. We have previously shown that electrospun biomimetic scaffolds that simulate the structure of native extracellular matrix, e.g., the nanoscalar fibrous nature of collagen, are effective in MSC-based skeletal tissue engineering, both in vitro and in vivo. We have recently custom-designed photocrosslinked hydrogel scaffolds derived from natural and synthetic polymers, to achieve live cell encapsulation during fabrication, with high fidelity tissue infrastructure reproduction and excellent cell retention, viability, and differentiation, and generated robust cartilage and bone tissue constructs.

Bioactivation of these biomimetic scaffolds by incorporating biologically targeted gene constructs results in transduction of both exogenous and endogenous host cells. These constructs may also be formed in situ, serving to both deliver cells and create custom-designed shapes for joint cartilage re-surfacing, potentially amenable to minimally invasive, arthroscopic procedures. Most recently, we have applied 3D-printing approach and a custom-designed microbioreactor to fabricate an MSC-derived microtissue analogue of the biophasic osteochondral junction of the articular joint,

demonstrating functional biological crosstalk between the chondral/osseous components. This osteochondral microphysiological system is currently being used to model the pathogenesis of osteoarthritis, e.g., exposure to pro-inflammatory agents, and to study biological, hormonal, pharmacological, and mechanical incluences on osteochondral health. Adult stem cells, with their multi-differentiation potential and their recently discovered trophic activities, in combination with biomimetic scaffolds, present a powerful platform for regenerative, therapeutic, and disease modeling applications in biomedicine. [Support: Pennsylvania Department of Health, NIH, Dept Defense, EPA]

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Kursad Turksen - Editor in Chief, Stem Cell Reviews and Reports Claudin 6 and epidermal differentiation: Use of animal models

Skin is one of the largest organs of the body, and is formed during development through a highly orchestrated process involving mesenchymal-epithelial interactions, cell commitment, and terminal differentiation. It protects against microorganism invasion and UV irradiation, inhibits water loss, regulates body temperature, and is an important part of the immune system. Using transgenic mouse technology, we have demonstrated that Claudin (Cldn)-containing tight junctions (TJs) are intricately involved in cell signaling during epidermal differentiation and that an epidermal

suprabasal overexpression of Cldn6 results in a perturbed epidermal terminal differentiation program with distinct phenotypic abnormalities. To delineate the role of the Cldn cytoplasmic tail domain in epidermal differentiation, we engineered transgenic mice targeting the overexpression of a Cldn6 cytoplasmic tail-truncation mutant in the epidermis. Transgenic mice were characterized by a lethal barrier dysfunction in addition to the existence of hyperproliferative squamous

invaginations/cysts replacing hair follicles. Immunohistochemical analysis revealed an epidermal cytoplasmic accumulation of Cldn6, Cldn11, Cldn12, and Cldn18, downregulation of Cldn1 and aberrant expression of various classical markers of epidermal differentiation; namely the basal keratins as well as K1, involucrin, loricrin, and filaggrin. Collectively these studies suggest an important role for Cldns in epidermal/hair follicle differentiation programs likely involving cross talk to signaling pathways (e.g., Notch) directing cell fate selection and differentiation