V-K1 0.422 Pembe-viole 0.200 Pembe-viole V-K2 0.433 Pembe-viole 0.211 Pembe-viole V-K3 0.433 Pembe-viole 0.211 Pembe-viole V-Ç 0.422 Pembe-viole 0.200 Pembe-viole N 0.433 Pembe-viole 0.211 Pembe-viole C 0.433 Pembe-viole 0.211 Pembe-viole S 0.433 Pembe-viole 0.200 Pembe-viole
TARTI MA
Bu çal mada; aktarlarda “kedi otu” ismi ile sat lan örnekler Tar m ve Köy i leri Bakanl ndan izinli sat lan kök, çiçek ve kapsül formu ile Sa k bakanl ndan izinli tablet ve draje formlar ndaki preparatlar ile kar la lm r.
Aktardan ald z “kedi otu” ad alt nda sat lan numuneler ile bakanl k onayl olarak sat lan kökler makroskobik olarak incelendi inde soluk kahverengi, grimsi, silindirik, yakla k 50 mm uzunluk ve 30 mm çap nda oldu u saptanm r. Bu görünü özellikleriyle Avrupa Farmakopesi’nde belirtilen tan mlamalara uygunluk göstermi tir.
Örnekler toz edildi inde, Farmakopede belirtildi i gibi soluk ye ilimsi, kahverengi renkte görülmektedir. Mikroskop alt nda toz numuneler kloral hidrat ve sartur reaktifleri ile incelendi inde, damlac klar halinde uçucu ya , dar ve kal n duvarl sklerenkima görülmü tür. Sartur rektifi ile , tek tek ya da gruplar halinde mavi-mor renkte ni asta taneleri görülmü tür. Ni astalar su ile incelendi inde hilumlar n çatlak oldu u saptanm r.
Aktar örnekleri, Tar m ve Köy leri ile Sa k Bakanl onayl di er numunelerdeki valerenik asit ve asetovalerenik asitin varl n tespiti için nce tabaka kromatografisinden yararlan lm r ve dört farkl solvan sistemi kullan larak Rf de erleri hesaplanm r.
Glasial asetik asit: etil asetat: siklohekzan (2:38:60) solvan sistemi kullan ld nda örneklerimizde valerenik asitin Rf de erleri 0.633- 0.677 ve asetoksi valerenik asit Rf de erleri 0.516-0.525 olarak
saptanm r. Bu de erler referansta belirtilen valerenik asit (Rf 0.657), asetoksi valerenik asit (Rf 0.514) de erlerine uygunluk göstermi tir. 90
Denedi imiz ikinci solvan sisteminde Etilasetat: hekzan (30:70) solvan sistemi kullan ld nda valerenik asitlerin Rf de erleri 0.485- 0.515 ve asetoksi valerenik asit Rf de erleri 0.281-0.312 oldu u görülmü ve referansta belirtilen valerenik asit (Rf 0.483), asetoksi valerenik asit (Rf 0.290) de erlerine uygunluk göstermi tir92.
Glasial asetik asit: etil asetat: hekzan (5:35:60) solvan sistemi kullan ld nda valerenik asit Rf de erleri 0.422-0.433 ve asetoksi valerenik asit Rf de erlerinin 0.200-0.211 aras nda oldu u ve referansta belirtilen valerenik asit (Rf 0.445), asetoksi valerenik asit (Rf 0.223) de erlerine uygunluk göstermi tir.93
Petroleteri:aseton(15:3) solvan sistemi kullan ld nda valerenik asitin Rf de erleri 0.302-0.322 görülmü ve literatürde belirtilen valerenik asit (Rf 0.453) de erine uygunluk göstermemi tir.29 Bu farkl n literatürde belirtilen solvan sistemindeki petrol eterinin k.n 40-50 0C iken uygulamam zda kulland z petrol eterinin k.n 80-100 0C olmas ndan kaynakland kan na var lm r.
Çal mam zda valerenik asit ve asetoksi valerenik asitin plak üzerinde belirlenmesi için Avrupa Farmakopesinde belirtildi i gibi anisaldehit-sülfürik asit reaktifi kullan lm ve s cakl n etkisi ile pembe- viole rengin meydana geldi i saptanm r.
Numunelerin kurutmadaki kay plar %10-11 aras nda bulunmu ve Avrupa Farmakopesinde en fazla %12 olarak belirtilen de erden az ç karak uygunluk göstermi tir.
Total kül miktarlar da %9-11 olarak saptanm ve Farmakope’de en fazla %12 olarak belirtilen de ere uygunluk göstermi tir. Asitte erimeyen kül miktarlar ise , örneklerdeki %3-4,5 kül de erleri Farmakopede önerilen en fazla %5 olarak gösterilen de ere uygun oldu u saptanm r.
Örneklerdeki uçucu ya miktarlar %0.1-0.3 olarak bulunmu tur . Bu de erlerler de Farmakopeye uygunluk göstermi tir.
SONUÇ
Çal mam zda, aktarlarda “kedi otu” ismi ile sat lan örneklerin Tar m ve Köy i leri Bakanl ndan izinli sat lan kök, çiçek ve kapsül formu ile Sa k Bakanl ndan izinli tablet ve draje formlar ndaki preparatlar na uygulanan deney sonuçlar ile Avrupa Farmakopesi analiz de erleri kar la ld nda, tüm ürünlerin Farmakope de erlerine uygunluk gösterdi i görülmü tür. Yabanc madde miktar da Farmakope ile uygunluk göstermi tir.
Aktarlarda kedi otu ad ile sat lan ürünlerde tah at yap lmad görülmü tür.
7.ÖZET
Valeriana türleri uzun y llardan beri halk t bb nda sedatif olarak kullan lmaktad r. Valeriana türlerinden kedi otu, kaya iple i, çoban kam isimleri ile bilinen türü olan Valeriana officinalis eczac kta en çok kullan lan türdür.
Bu çal mada; aktarlardan “kedi otu” ismi ile sat n al nan ruhsats z örnekler ile Tar m ve Köy i leri Bakanl ndan izinli olan biri çiçek di er ikisi kök olan örnekler ile Sa k Bakanl ndan izinli tablet, draje formundaki preparatlar kar la lm r.
Örneklerin kurutmada kay p miktarlar % 10.22-11.85; kül miktarlar % 9.22-11.85; asitte erimeyen kül miktarlar % 3.90-4.46; uçucu ya miktarlar % 0.1-0.3 olarak saptanm r. nce tabaka kromatografisi çal mas nda dört farkl solvan sistemi kullan larak örnekler valerenik asit ve asetovalerenik asitin Rf de erleri aç ndan de erlendirilmi ve Avrupa Farmakopesi (6.0)’a uygun olup olmad na bak lm r.
8.SUMMARY
Valeriana species have been used for a long time as sedative in folk medicine. Valeriana officinalis named “kediotu, kaya
iple i, çoban kam ” is used in therapy.
In this study; the plants sold at akthars as “Cat herb” were compared with root, flower and capsul forms that are permitted by Ministry of Agriculture and Rural Affairs. And also tablet and drage forms that are permitted by Ministry of Health.
Ash values are 9.22-11.85 %, ashes insoluble in acid are 3.90-4.46 %, losses on drying are 10.22-11.85 % and essential oils are 0.1-0.3 %.
Four different solvent systems were used for identification of valerenic acid and asetovalerenic acid by means of TLC. It is also investigated if they were suitable values that of European Pharmacopeia(6.0).
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10-ÖZGEÇM
Ad : CANER
Soyad : AN
Do um Yeri ve Tarihi: ANKARA,13.08.1980
itimi :
1999-2004 Gazi Üniversitesi Eczac k Fakültesi(Ankara) 1994-1998 Sincan Lisesi(Ankara)
1992-1994 Ahmet Andiçen Ortaokulu(Ankara) 1987-1992 Plevne lkokulu(Ankara)