Teflon artificial membranes and collagen membrane on wound healing phenomena of cell adhesion and cytokine expression and comparison of observed
Abstract
After periodontal treatment, we hope to periodontal regeneration technology to restore the function of periodontal tissue. In periodontal surgery been widely adopted, is to ePTFE (expanded polytetrafluoroethylene) is made of GORE-TEX ® (Goretex) membrane, but there is need to use this membrane to the second surgery and prone to patch exposed distress caused by infection. Biomend
Extend™ (Biomend) is an absorbable collagen membrane in clinical use have good results; and prepared by our laboratory pig skin collagen membrane (porcine dermal collagen membrane; PDCM), is pig's dermis layer to a refined absorbable collagen membrane, studies show good biocompatibility. As the
diaphragm and the role of organizational integration between the organization and the immune response tends not yet fully understood, this experiment, SD rat as the experimental object, PDCM, Biomend with Goretexpatch implanted in the animal skin, aim to observe the three types of diaphragm and organization of adhesion molecule expression interface and a set of strength test system, by Kruskal-Wallis test and Mann-Whitneymultiple comparisons, the analysis of the immune response of three artificial membrane trend. The results showed that the three diaphragms, Goretexmembrane surface adhesion molecules in
epithelial tissues have a stronger reaction and weak α 2 β4expression, showing a high favorable trend of moving epithelial cells may lead to clinical Goretex exposure after patch is easy in the circumstances; in connective tissue and the diaphragm at the junction part of the only PDCM α5 can be found in the reaction.
While in the first 7days, 10 days and 14days between the observed interface patch and tissue immune response generated by the trend analysis revealed three types of diaphragms, the period of interleukin-2 (IL-2) expression no
statistically significant differences in the interferon-γ (INF-γ) in the performance of the three membranes and no obvious difference. ThePDCM patch at all times in theTh2 type induced the interleukin-4 (IL-4), interleukin-10 (IL-10) and
interleukin-13 (IL-13) expression were lower than the other two membranes, especially the first 7days to 14 days were significantly lower than the Goretex performance (P <0.05). More IL2/IL4ratio, has been found PDCMdiaphragm and the control group than the similar performance; the Goretexwas significantly lower than the control group (P <0.01), so showGoretex in the tissue caused by
theTh1/Th2 response, resulting inGTR techniques tend to tissue damage. This
study indicate that: Goretex membrane epithelium caused by a higher tendency to move, may be the cause of clinical GTR in periodontal applications of
technologies leading to membrane exposure causes Zeyi PDCM in the connective tissue reaction with α 5; of PDCM membrane in the wound-induced cytokine expression, more than Biomend with Goretexmembrane performance towards Th1 cytokines should be able to guide the organization toward repairing the state of development.
English Abstract
