Book of Abstracts of the 68th
Annual Meeting of the European
Federation of Animal Science
Book of abstracts
No. 23 (2017)
EAAP – 68
thAnnual Meeting, Tallinn 2017
53
Session 31. Biology of adipose tissue and lipid metabolism
Date: Wednesday 30 August 2016; 8.30 – 12.00
Chair: I. Louveau
Theatre Session 31
The biology of adipose tissue in non-ruminants: renewed interests in oxido-reduction pathways 302
F. Gondret, M.-H. Perruchot, K. Sierzant and I. Louveau
Proteomics profiling of cow adipose tissue in relation to energy balance around calving 303
C. Piras, A. Soggiu, I. Alloggio, V. Greco, L. Bonizzi, A. Urbani and P. Roncada
Regulation of 17bHSD12 mRNA expression in adipose tissue of dairy cows with different body condition 303
K. Schuh, S. Häussler, C. Urh, C. Koch, D. Frieten, G. Dusel, H. Sadri and H. Sauerwein
Identification and characterization of adipokines and myokines involved in fat deposition in cattle 304
E. Albrecht, L. Schering, Y. Liu, K. Komolka and S. Maak
Adiposity and expression of lipid metabolism genes in skeletal muscle in two beef cattle breeds 304
O. Urrutia, A. Arana, B. Soret, L. Alfonso, A. Purroy and J.A. Mendizabal
Immune stimulation in adipose tissue and its consequences for health and performance in the pig 305
K. Ajuwon
Adipose-immune cross-talks in two pig lines divergently selected for feed efficiency – part 1 305
M.H. Perruchot, E. Merlot, I. Louveau and N. Le Floc’h
Effect of two different omega-3 fatty acid sources in the prepartum diet on performance of calves 306
A. Ataozu, F.E. Gursel, K. Oztabak, L. Celik and U. Serbester
Cholesterol metabolism and performance in Holstein cholesterol deficiency carrier dairy cows 306
A.-C. Schwinn, J.J. Gross, C. Drögemüller, R.M. Bruckmaier, F. Schmitz-Hsu, A. Barenco and T. Neuenschwander
Poster Session 31
Association of gene expression of ASIP and its receptors with fat deposition in cattle 307
Y. Liu, E. Albrecht, L. Schering and S. Maak
Effect of monensin on milk fatty acid profile and blood metabolites in transition dairy cows 307
P. Karis, M. Henno, T. Ariko, H. Jaakson, K. Ling and M. Ots
Relationship between major muscle constituents in longissimus muscle from Hungarian Simmental bulls 308
G. Holló, B. Húth, I. Anton and I. Holló
Influence of carnitine and fat on performance, carcass characteristics and serum lipids of broilers 308
M. Bouyeh and H. Akhondzade
Expression of perilipin 2 and leptin genes in muscle and backfat tissues of pigs 309
K. Tempfli, K. Szalai, E. Lencses-Varga, Z. Simon and A. Bali Papp
Adipose-immune cross-talks in two pig lines divergently selected for feed efficiency – part 2 309
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EAAP – 68
thAnnual Meeting, Tallinn 2017
Session 31
Theatre 9
Session 31
Theatre 8
Cholesterol metabolism and performance in Holstein cholesterol deficiency carrier dairy cows
A.-C. Schwinn1, J.J. Gross1, C. Drögemüller2, R.M. Bruckmaier1, F. Schmitz-Hsu3, A. Barenco4 and T.
Neuenschwander5
1Veterinary Physiology, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3012 Bern, Switzerland, 2Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3012 Bern, Switzerland, 3Swissgenetics, Meielenfeldweg 12, 3052 Zollikofen, Switzerland, 4Swissherdbook cooperative, Schützenstrasse 10, 3052
Zollikofen, Switzerland, 5Holstein Association of Switzerland, Route de Grangeneuve 27, 1725 Posieux, Switzerland;
ann-catherine.schwinn@vetsuisse.unibe.ch
The recently detected cholesterol deficiency (CD) in Holstein cattle is associated with reduced cholesterol in calves and bulls and caused by a loss of function mutation of the APOB gene. We set out to investigate if this mutation influences also the metabolism and performance of dairy cows. Twenty pairs of full sibling cows from same farms were investigated. Each pair contained a heterozygous carrier (CDC) and a non-carrier (CDF) of the disease associated APOB mutation. Blood samples were taken at 3-4 weeks and 4-6 months in lactation to measure cholesterol metabolites. A paired t-test was used to compare both groups. Total and free cholesterol (TC), high density lipoproteins, low density lipoproteins, phospholipids and cholesterol esters, but not triacylglycerides and very low density lipoproteins were lower in CDC cows compared with matched CDF siblings at both time points (P<0.05). Concentrations of glucose, free fatty acids, beta-hydroxybutyrate, and aspartat-aminotransferase and gamma-glutamyl-transferase activities did not differ between both groups (P>0.05). Milk yield, milk protein and milk fat in the previous and current lactation as well as days open did not differ between groups (P>0.05). Although cholesterol and associated fractions were reduced in heterozygous carriers of the CD causing APOB mutation, performance within matched sibling pairs did not differ in both early and mid-lactation.
Effect of two different omega-3 fatty acid sources in the prepartum diet on performance of calves
A. Ataozu1, F.E. Gursel2, K. Oztabak2, L. Celik1 and U. Serbester1
1Cukurova University Agricultural Faculty, Department of Animal Science, Adana, 01330, Turkey, 2Istanbul University
Veterinary Faculty, Department of Biochemistry, Istanbul, 34320, Turkey; ugurserbester@gmail.com
Flaxseed oil (FOS) and fish oil (FO) contain omega-3 fatty acids. Flaxseed oil is characterized by a high concentration of alpha linolenic acids (ALA) while fish oil is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). ALA can be converted through a series of desaturation and elongation reactions into EPA and DHA. However, these reactions have a poor efficiency in mammals. Therefore, the hypothesis was that maternal omega-3 fatty acids would influence differently gestation length, birth weight, weaning weight and average daily gain, and blood parameters of calves. Twenty five multiparous Holstein cows were used in an incomplete randomized block design with repeated measurements during summer 2016. Cows were blocked according to parity and last lactation milk yield and were randomly assigned to 1 of 2 prepartum total mixed ration supplemented with 5.4% FO or FOS during the last 21 d of gestation. Calves were fed colostrum within 2 h of birth from their own dam or from a dam fed the same treatment. After the colostral period, they were fed 4 L of whole milk and ad libitum alfalfa hay until weaning. Body weights were measured weekly, blood samples were taken at biweekly intervals. There were no difference in gestation length (274.5 vs 272.0 d for FO and FOS, respectively) and body weight at partum (594.6 vs 593.5 kg for FO and FOS, respectively) among the groups. Omega-3 fatty acid sources did not affect birth weights of calves (36.6 vs 34.1 kg for FO and FOS, respectively). Also, the source of omega-3 fatty acids in the diets did not influence the mean serum concentrations of alanine aminotransferase, aspartate aminotransferase, gamma-glutamyltransferase, alkaline phosphatase, glucose, total cholesterol and triglycerides. The results indicate that inclusion of FO or FOS to prepartum diet shows similar effect on the calf performance including metabolic profile.
