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魚油對敗血症小鼠發炎反應及粒線體功能之影響 李正中

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魚油對敗血症小鼠發炎反應及粒線體功能之影響 李正中 邱琬淳 葉松鈴 台北醫學大學保健營養學系 本研究探討飲食中添加不同劑量之魚油對敗血症小鼠粒線體功能及發炎反應之影 響。實驗將小鼠分成三組,分別給予含10 %大豆油的控制組(C)、2.5 %魚油混合7.5 %大豆油組(LF)及7.5 %魚油混合2.5 %大豆油組(HF),餵養三週後進行盲腸結紮穿 刺手術(cecal ligation and puncture, CLP)引致敗血症,並於第0、6、及24小時犧牲動 物,取其腹腔沖洗液及肝臟進行分析。結果顯示於CLP後6小時及24小時HF組其腹 腔沖洗液中前列腺素E2及腫瘤壞死因子-α皆顯著低於C和LF組。CLP後6小時,HF 組肝臟粒線體酵素NADH cytochrome c reductase, succinate cytochrome c reductase 及cytochrome c oxidase活性皆顯著高於C組,CLP後6小時,各組肝臟粒線體型態均 有腫大的現象,且以LF組最為明顯,故飲食中含較高比例之魚油可於敗血症後降 低發炎反應並維持肝臟粒線體酵素活性。

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