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Limit of Linear Response (LOL): The saturation point of the detector of an instrument ; after that point it can not produce a linear response for the signal.

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(1)

Limit of Linear Response (LOL):

The saturation point of the

detector of an instrument ; after

that point it can not produce a

linear response for the signal.

(2)

CALIBRATION METHODS

*Calibration curve method

*Standard addition method

*Internal standard method

*Area normalization method

(3)

1. Calibration Curve Method:

To be able to use the calibration curve, the concentration range of the analyte should be

estimated, eg matrix conditions should be evaluated.

(4)

* A set of standards is prepared, for example, in conditions similar to

matrix conditions; standards should be at levels that cover the

estimated concentration range.

(5)

* An analytical reference is prepared. * Standards and

reference are measured in the

analyzer and a calibration graph is

drawn with the obtained values.

(6)

* Normally, at least 3 standards and

one reference are used to draw a

calibration curve.

(7)

* For example (the analyte

concentration is unknown) is

measured; the concentration is

calculated within the dynamic

range of the received response

calibration curve.

(8)

Calibration curve

Absorbance

Concentration g/L

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