beta-chitin 作為生物感測器之 應用及其相關研究

殼糖是一種身體可吸收性材料，具有生體可相容性及生體可降解性，並 且經由一些體內的酵素分解產生中間產物具有免疫賦活性。依造殼糖的結構 分為三種，其中 a- 及 b- 兩種最為人所知。以往以 a-chitin 為對象，本研究利 用 squid bone 經由脫鈣脫蛋白的過程精製 b-chitin 。精製時的濃度、溫度越高

，會因為主鏈斷鍵以及分子結構改變造成分子量降低。利用 5 % LiCl / DMAC 製成殼糖溶液並紡絲成膜，以 4 % 之殼糖溶液所製成之成形物張力強度最強。

在鹼性環境下，利用高溫將殼糖轉變為聚葡萄胺醣，可應用在組織工程學上

，作為人工材料使用。利用 X-ray 繞射儀，可依結晶度比較兩種 Type 之差異。

細胞毒性測試，利用其他材料與殼糖膜相比較，殼糖膜之 O.D 值分別為 115

% 及 104 % ，與其他材料相比，有高度的免疫相容性，適合應用在動物體。

材料熱性質測試，比較殼糖 CM 化前後之差異， CM 化殼糖在 100°C 、 217

°C 左右產生明顯波峰，可依此特性與未 CM 化殼糖相比較。 FT-IR 光譜判定

，精製殼糖在 IR 光譜上產生其特徵吸收峰； CM 化之殼糖膜，利用 FT-IR 之 ATR 照射，可觀察 1700cm-1 有 -CO 官能基產生。經由酵素固定過程，製成 酵素殼糖膜作為生物感測器之基質，且進行活性測試，利用分光光度計，可 測得 1cm2 之殼糖膜表面，殘存酵素約 0.2 — 0.3 units 。未來並希望酵素殼糖 膜，在生物感測器有更好的發展。

beta-chitin 作為生物感測器之 應用及其相關研究

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Chitin, one of the most abundant polymers in Nature, is a biocompatible and biodegr able polymer. In the body, chitin can be digest through by enzyme and is a low immu nogenetic poly-saccharide. Chitin can be separated in three types by its structure, wit h a-and b- types well known. To investigate a-chitin used, in this proposed research, we will apply the technique of wet spinning or casting to develop a facile type of chit in fiber and membrane from the chitin dope, which prepared with high molecular wei ght shrimp-resource chitin dissolved in metal salt / amine-containing solvent. At high ly temperature and concentration, have low molecular weight .The stress is highly at 4% chitin solution. The chitosan scaffolds derived from chitin in the strong base and high temperature bs used for tissue engineering use. In the X-ray diffraction pattern o f chitin, some differences were observed between a- and b- types. In the MTT assay, we can find chitin is biocompatible and bioabsorbable. In the thermal analysis, rH of carboxymethylchitin found at temperature 217°C different from other types. We also find the characteric absorbant peak by FT-IR . After enzyme immobolization of chiti n membranes, found the residue activity about 0.2-0.3 units at 1 cm2 membrane by t he spectrumeter. We hope that the uses of carboxymethylchitin for enzyme immobliz ation, in the future, can have a good application at the biosensor.

Application of beta-chitin for biosensor matrix use