beta-chitin 作為生物感測器之應用及其相關研究
Application of beta-chitin for biosensor matrix use
中文摘要殼糖是一種身體可吸收性材料，具有生體可相容性及生體可降解性，並且經由一 些體內的酵素分解產生中間產物具有免疫賦活性。依造殼糖的結構分為三種，其 中a-及 b-兩種最為人所知。以往以 a-chitin 為對象，本研究利用 squid bone 經由脫鈣脫蛋白的過程精製b-chitin。精製時的濃度、溫度越高，會因為主鏈斷 鍵以及分子結構改變造成分子量降低。利用5 % LiCl / DMAC 製成殼糖溶液並 紡絲成膜，以4 %之殼糖溶液所製成之成形物張力強度最強。在鹼性環境下， 利用高溫將殼糖轉變為聚葡萄胺醣，可應用在組織工程學上，作為人工材料使 用。利用X-ray 繞射儀，可依結晶度比較兩種 Type 之差異。細胞毒性測試，利 用其他材料與殼糖膜相比較，殼糖膜之O.D 值分別為 115 %及 104 %，與其 他材料相比，有高度的免疫相容性，適合應用在動物體。材料熱性質測試，比較 殼糖CM 化前後之差異，CM 化殼糖在 100°C、 217°C 左右產生明顯波峰，可 依此特性與未CM 化殼糖相比較。FT-IR 光譜判定，精製殼糖在 IR 光譜上產生 其特徵吸收峰；CM 化之殼糖膜，利用 FT-IR 之 ATR 照射，可觀察 1700cm-1 有-CO 官能基產生。經由酵素固定過程，製成酵素殼糖膜作為生物感測器之基 質，且進行活性測試，利用分光光度計，可測得1cm2 之殼糖膜表面，殘存酵 素約0.2 — 0.3 units。未來並希望酵素殼糖膜，在生物感測器有更好的發展。 英文摘要
Chitin, one of the most abundant polymers in Nature, is a biocompatible and
biodegrable polymer. In the body, chitin can be digest through by enzyme and is a low immunogenetic poly-saccharide. Chitin can be separated in three types by its structure, with a-and b- types well known. To investigate a-chitin used, in this proposed research, we will apply the technique of wet spinning or casting to develop a facile type of chitin fiber and membrane from the chitin dope, which prepared with high molecular weight shrimp-resource chitin dissolved in metal salt / amine-containing solvent. At highly temperature and concentration, have low molecular weight .The stress is highly at 4% chitin solution. The chitosan scaffolds derived from chitin in the strong base and high temperature bs used for tissue engineering use. In the X-ray diffraction pattern of chitin, some differences were observed between a- and b- types. In the MTT assay, we can find chitin is biocompatible and bioabsorbable. In the thermal analysis, rH of carboxymethylchitin found at temperature 217°C different from other types. We also find the characteric absorbant peak by FT-IR . After enzyme
1 cm2 membrane by the spectrumeter. We hope that the uses of carboxymethylchitin for enzyme immoblization, in the future, can have a good application at the biosensor.
