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Chromatography using for: Analyze – examine a mixture, its components, and their relations to one another

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Academic year: 2021

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(1)

Chromatography using for:

Analyze – examine a mixture, its

components, and their relations to one another

(2)

•Identify – determine the identity of a mixture or components based on known components

(3)

•Purify – separate

components in order to isolate one of interest for further study

(4)

•Quantify – determine the amount of the a

mixture and/or the

components present in the sample

(5)

Chromatogram:

It is the visual output

of the

chromatograph.

(6)

Chromatograph:

It is equipment that enables a sophisticated

Separation.

(7)

Stationary phase:

It is a phase that is covalently bonded to the support particles or to the inside wall of the column tubing.

(8)

Eluent:

It is the solvent that will carry the analyte.

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