BIO 440
MICROBIAL
BIOTECHNOLOGY
B- GENETIC ENGINEERING B- GENETIC ENGINEERING
and RECOMBINANT and RECOMBINANT
PRODUCTS
PRODUCTS
• Genetic engineering: The use of laboratory techniques in DNA isolation, manipulation and expression, as well as in the development of genetically modified organisms (GMOs).
• Biotechnology: The use of GMO organisms to carry out certain chemical processes for use in industrial applications.
Genetic engineering techniques
• Restriction and modification systems and usage areas of restriction endonucleases
• Nucleic acid hybridization
• Southern blot
• Finding the right clone with DNA probe
• Region-specific mutagenesis
• Cassette Mutagenesis
• Polymerase chain reaction
• DNA sequence analysis
• Complementary DNA (cDNA) synthesis
• Observing gene expression with green fluorescent protein (GFP)
• DNA gun for transfection of eukaryotic cells
• Genetic engineering techniques
• Hosts for Cloning Vectors
• Finding the Right Clone
• Specialized Vectors
• Expression of Mammalian Genes in Bacteria
• Practical applications of genetic engineering
• Insulin Production: The Beginning of Commercial Biotechnology
• Other Mammalian Proteins and Products
• Genetically Engineered Vaccines
• Practical applications of genetic engineering
• Genetic Engineering in Animal and Human Genetics
• Genetic Engineering in Herbal Agriculture:
• Transgenic Plants
