Biogenic amine formations in wet and spray-dried fish (Equulites klunzingeri) silage prepared with lactic acid bacteria strains

f*togemir:

amline

formatioms

[n

lvet

amd

spnay-dried

tish

_{Hquutites

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sitrage

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rryith

lactflc

acid

hacteria

stnatns

Gulsun Ozyurtu, Ali Serhat Ozkutuk"., Esmeray Kuley Boga", Mustafa Durmusu, Fatih Ozogul" "Cukurova University

*aliserhat@cu.edu.tr Abstract:

Fish silage is an effective method

to

produce high quality animal food from discard fish. Lactic acid bacteria utilization for the production of fish silages have considerable advantages than the chemical silages because of their positive properties such as organic acids, bacteriocins productions and the

enhancement

of

the flavour. However, fish products may contain biogenic amines because of the action

of

endogenous and bacterial decarboxylase enzymes activities.

lt

was reported

that

some lactic acid bacteria (LAB) were able to degrade biogenic amines by means of amino oxidases. ln this

study, the effects of lactic acid bacteria on biogenic amines in wet and spray-dried fish silages for the duration of the fermentation process were investigated.

The

fish

(Equulites klunzingeri) silages were prepared

with

LAB strains (Lactobacillus plantarum, Lactobacillus brevis, Pediococcus acidilactici, Enterococcus gallinarum and Streptococcus spp.) and

formic

acid as

the

control

group.

Acid and

fermented silage groups

were stored

at

room

temperature (27-28oC\ for three weeks. After three weeks, all silage groups were spray-drieri L;siiig

mini spray dryer (Buchi-290,Switzerland). Biogenic amine analysis in wet and dried fish silages was

performed using a rapid HPLC method. This project was supported by Scientific and Technological Resea rch Council of Turkey (TOVAG-2 13O166).

Putrescine, cadaverine, seratonine, tyramine, dopamine and agmatine were

the

main biogenic

amines in all silage groups. The initial putrescine and cadaverine content were found as 1.11 and

1.99 mg/100g, respectively. There were considerable increase

in the

contents

of

these biogenic amines at the end of the fermentation process for all groups (4.15

to

L3.15mg/100g for putrescine

and 8.91

to

4L.37mghOOg

for

cadaverine).

After

spray-drying,

the

main biogenic amines were putrescine (1.7L-4.06mc/100s), cadaverine (3.45-1L.30mg/fOOg) and tyramine (0.07-L.99mg/100g).

The results show

that

biogenic amine concentrations in wet and spray-dried fish silages prepared

with acid and LAB strains had no adverse effects to animal health.

Keywords: Biogenic amines, lactic acid bacteria, Equulites klunzingeri, discard fish, fish silage