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A hybrid nanofiber matrix to control the survival and maturation of brain neurons

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Fig. 1. PA and collagen molecules interact when two solutions are mixed. (A) Structure of branched PA molecules presenting bioactive peptide sequence IKVAV (left), or IKVAV and YIGSR (right)
Fig. 3. Attachment and survival of cerebellar neurons on the hybrid matrix. (A) Dissociated cerebellar cells were seeded on the surface of various gel substrates, and examined after 2.5 h under phase contrast microscopy to assess cell attachment
Fig. 4. Effects of matrix composition on Purkinje cell (PC) morphology. (A) SFP (simulated fluorescence process) volume rendered images of PCs (Calbindinþ, green) at 16 DIV, cultured on either collagen (upper, side view) or collagen/IKVAV-PA (“IK-PA”, 1.0 m
Fig. 5. Mature PCs on a hybrid matrix form synaptic connections and show electrical activity

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