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atherosclerosis, glutathione peroxidase (GPX-1) is one of the defense mechanisms, protecting the vascular system against oxidative stress. This process is supported by the GPX-1 expression in the blood vessels. It has been stated that low GPX-1 activity results in a higher incidence of cardiovascular anomalies, and that increased GPX activity could have potential therapeutic effects. In association with GPX activity, the c.C599T polymorphism leading to a change from proline to leucine at protein position 198 (p.Pro198Leu), is suggested to be an early diagnostic parameter for coronary artery diseases (CAD). The purpose of this study was to investigate the c.C599T polymorphism in Turkish patients with CAD. METHODS: We have investigated 113 volunteers, composed of CAD patients and healthy control volunteers. The CAD samples were collected at the Marmara University, Department of Cardiovascular Surgery. Peripheral blood samples (10 ml) were collected from subjects with no clinical evidence of type 2-diabetes. Genomic DNA was isolated with the salt precipitation method, polymerase chain reaction, restriction fragment length polymorphism and agarose gel electrophoresis techniques were utilized. This study was approved by the Ethical Review Board of Istanbul Medical Faculty, Istanbul University.RESULTS: The CC, TT, CT, genotypes of the control and CAD groups were, 23.8%, 54.8%, 21.4% and 15.5%, 12.7%, 71.8%, respectively. A significantly increased risk for developing CAD was found for the group bearing the heterozygous CT genotype (p<0.001).
CONCLUSION: These preliminary results, provide new evidence that Turkish patients with CAD have a tendency to be heterozygous for
c.C599T/p.Pro198Leu. Suggesting that heterozygosity could be an early diagnostic parameter, in contrast to other populations where the C allele is predictive for CAD. Further research is required to determine therapeutic effects of increased GPX activity in the Turkish population. This project was supported by IU BAP (Project no: 21596).
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The Effect of Resvaratrol on Oxidative Stress in Diabetic Rat Heart Tissue
Çiğdem Özer1, Burcu Sırmatel1, Duygu Tozcu2 1Gazi University, Faculty of Medicine, Department of Physiology, Ankara, Turkey
2Ufuk University, Fculty of Medicine, Department of Physiology, Ankara, Turkey
AIM: Resveratrol (RSV), a vegetable flavonoid, is known to have vasodilator, antihyperlipidemic, antiinflammatory, antiaggregant and cardioprotective effects. RSV reduces the risk of myocardial infarction by reducing Low Density Lipoprotein (LDL)
oxidation by the action of free radical scavengers. Its vasodilator effect decreases the risk of atherosclerosis
by blocking the increase in platelet aggregation induced by a hypercholesterolemic diet. In our study, we aimed to investigate the effects of resveratrol on oxidant and antioxidant systems in heart tissue in rats with streptozotocin (STZ) diabetic rats.
METHODS: After approval of the ethics committee 30 adult male Wistar albino rats were divided into 4 groups as follows:1.Control, 2.Resveratrol,
3.Diabetes, 4.Diabetes+Resveratrol. Streptozotocin STZ(65 mg/kg 0,1M in citrate buffer) were administered to diabetes groups,citrate buffer were administered to control groups intraperitoneally as a single dose. Application of RSV(10/mg/kg/day dose (dissolved in 0.1M ethanol) through 8 weeks by using oral gavage. At the end of experiments rats were anaesthetized with Rompun+ketamine(50+60- 100mg/ kg),and sacrificed by cardiac puncture. Heart tissue oxidant (Malondialdehit/MDA) and antioxidants (glutathione/GSH) parameters were studied. Results, One Way Anova and Fisher LSD tests were compared using, p<0.05 was considered significant. RESULTS and CONCLUSIONS: Diabetes-related GSH leves were decreased while MDA levels were increased in the heart tissues. The administered RSV caused a significant decrease in tissue MDA levels (p <0.05). Despite the increase in tissue GSH levels due to RSV, but was not significant. So RSV has reduced oxidative damage in diabetic heart tissue and has caused a slight increase in antioxidant levels. We believe that RSV may be useful in preventing complications of diabetes by the contribution of new studies.
Our study was supported by Gazi University Scientific Research Projects Unit (code: 01/2011-75) and the studies were carried out at Gazi University Faculty of Medicine Physiology Laboratory.
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Efect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats
Abdullah Özer1, Faruk Metin Çomu2, Ayşegül Küçük3, Yiğit Kılıç1, Barış Mardin1, Metin Alkan4, Gürsel Levent Oktar1, Yusuf Ünal4, Mustafa Arslan4 1Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, Turkey 2Department of Physiology, Kirikkale University Medical Faculty, Kirikkale, Turkey
3Department of Physiology, Dumlupinar University Medical Faculty, Kütahya, Turkey
4Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey AIM: Ischemia-reperfusion (IR) injury is a cascade of events initiated by tissue ischemia. The cellular damage caused by reperfusion leads to an active inflammatory response. Erythrocyte deformability and plasma viscosity are of crucial importance for the
