Abstracts / Journal of Biotechnology 305S (2019) S33–S88 S75 effect. Results showed that bacterial fractions of all selected strains
showed significant suppression of TNF-␣ production in LPS stimu-lated PBMC cells.
https://doi.org/10.1016/j.jbiotec.2019.05.259 PP-APR13-015
Isolation of Pseudomonas aeruginosa bacteriophage from municipal sewage P. Alexyuk∗, M. Alexyuk, A. Bogoyavlenskiy, Y. Moldakhanov, K. Akanova, V. Berezin Departmen of Antiviral protection, Scientific and Production Center for Microbilogy and Virology, Almaty, Kazakhstan
One of the main problems of current care of public health is the nosocomial infections. One of the most frequent causative agents of nosocomial infections is Pseudomonas aeruginosa. Resistance to antibiotics, disinfectants complicates treatment of infections caused by this pathogen. An alternative method of control can be phagotherapy.
The aim of the research was isolating bacteriophages capable of lysing clinical strains of Pseudomonas aeruginosa from municipal sewage. Bacteriophages were isolated from municipal sewage samples of the Shymkent city. A clinical strain, Pseudomonas aeruginosa 325, isolated from patients suffering from pyoinflam-matory processes was used as an indicator culture. Treated water samples were cocultivated together with Pseudomonas aeruginosa 325 during 24 hours at 37◦C. Determination of morphology of negative colonies and its selection were carried out by inoculation of the obtained phagolysates with Pseudomonas aeruginosa 325 on solid nutrient medium. Pure strains of bacteriophages were obtained by selecting single lysis plaques in a series of selective passages with the indicator culture. The level of lytic activity and titer of the bacteriophage strains were determined by the Appelman and Gracia methods.
Four bacteriophage strains were isolated capable of lysing Pseu-domonas aeruginosa 325 with a titer of 108–109 virus particles per ml.
https://doi.org/10.1016/j.jbiotec.2019.05.260 PP-APR13-016
Experimental induction of DM in rats and the role of bariatric surgery for the recovery of pancreatic cells
L.D. Hritcu∗, D. Borcea, L. Burtan
Department of clinics, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine of Iasi, Romania
The study was conducted in two stages: the first implied induc-ing diabetes mellitus in 20 young Wistar rats weighinduc-ing 150–200 g, feeding them human food high in vegetal fats, oleaginous seeds, simple and complex carbohydrates, sugars, lipids, fats and pro-teins for a period of 4 weeks, then weighing and administering them streptozotocin by intraperitoneal injection. Blood sugar lev-els were monitored for 3 days and efforts were made to keep them within the normal range by administering hypoglycemics. Since blood sugar levels were still high (250–320 mg/dl), hypoglycemics were stopped and insulin was administered for balance. After 2–3 days, blood sugar levels were close to normal. The second stage
started after establishing the insulin dose and implied the par-tial gastrectomy of a 1.5–2.0 cm wide and 6.5–7.5 cm long area on the large curvature. After 1–2 weeks, rapid improvements in body weight and blood sugar control were observed. Consequently, a week afterwards, the insulin doses were lowered and 30 days later insulin was no longer necessary. In conclusion, after feeding the rats human food and administering them STZ, the induction of type 1 diabetes was successful and partial gastrectomy lead to a better blood sugar control.
https://doi.org/10.1016/j.jbiotec.2019.05.261 PP-APR13-017
Resolvin E1 increases mineralized tissue associated genes and mineralization of the cementoblasts
S. Bozkurt1,∗, S. Hakki2, A. Kantarci3
1Research Center of Dental Faculty, Selcuk
University, Konya, Turkey
2Department of Periodontology, Faculty of Dentistry,
Selcuk University, Konya, Turkey
3The Forsyth Institute, Boston, MA, USA
Resolvin E1 (RvE1) reverses the experimental periodontitis in animal studies. However, the mechanism of RvE1 on cemento-genesis is unknown. The aim of this study was to identify the impact of RvE1 on the bioactivity of the cementoblasts (OCCM.30). Bone sialoprotein (BSP), collagen type I (COL-I), osteocalcin (OCN), runt-related transcription factor 2 (Runx2) and alkaline phos-phatase (ALP) mRNA expression were assessed via RT-PCR at 24 and 72 hours. All concentrations of RvE1 resulted in a significant increase of ALP expression after 24- and 72-hour incubations. While all concentrations of RvE1 stimulated OCN and Runx2 mRNA expressions at 24 hrs, 10, 50 and 100 ng/ml concentrations induced OCN and Runx2 transcripts at 72 hrs. BSP expression in response to RvE1 was upregulated at 50 and 100 ng/ml RvE1 concentrations after 24 hours while all RvE1 concentrations stimulated BSP expres-sion at 72 hours. Increased COL-I mRNA expresexpres-sion was noted at 10, 50 and 100 ng/ml concentrations of RVE1 at both time points. All concentrations of RvE1 resulted in increased biomineralization. The present data suggested that RvE1 induces formation of the new cementum and its mineralization, both of which are critical for periodontal tissue regeneration.
Acknowledgments
Selcuk University Scientific Research Projects BAP17401164.
https://doi.org/10.1016/j.jbiotec.2019.05.262 PP-APR13-018
Atypical outbreak of myxomatosis in domestic rabbits in Eastern Romania
F. Bocaneti Daraban∗, A.M. Dascalu, E. Velescu, O.I. Tanase
Department of Public Health, Faculty of Veterinary Medicine, University of Agriculture Sciences and Veterinary Medicine, Ion Ionescu de la Brad, Iasi, Romania
Myxomatosis is an important disease in rabbits, caused by DNA virus, myxomavirus, genus Leporipoxvirus, family Poxviridae. Two
