Effects of curcumin supplementation on serum fetuin-a levels in rats fed a high-fat diet: Is curcumin useful for treatment of fatty liver disease?

M367

RoLE oF V|TAM|N D AND

FoLlc AclD

lN

cANcER

PREVENTloN

M. Oliveira Rodrfguez(1), P. Garcia Teijido(2), R. Venta Obaya(rI

1Depaıtment _{of Biochemistry, San Agustin Hospitat, Aviles,}

Asturias

2Oncoüogy _{Service, San Agustin Hospitat, Avil6s, Asturias}

Background: Experimental and epidemiological evidences have shown that some components of Western diets are

important factors in the development of colorectal cancer (CRC). ln this way, vitamin D (VitD) and folate have emerged as promising chemopreventive agents. The purpose of this

study was to evaluate the prevalence of VitD and folate deficiency

in

patients with initial diagnosis

of

CRC

and

compare the results with patients with other gastrointestinai

diseases.

Methods: Between November 2010 and September 2012,

individuals diagnosed of

CRC

and patients with colorectal adenomas, both diagnosed by colonoscopy and histologically confirmed, and controls that received negative colonoscopy results were enrolled

in

the study. Subjects consuming

supplements of vitamins were excluded. 75 patients with CRC (median 69, range from 45 to g0 years), 45 individuals with

adenomas (median 71, range from 53 to 89 years) and 52

controls (median 73, range from

31 to 92

years) were collected. Serum VitD and folate levels were measured by a

Roche Diagnostics automatic immunoassay for the Cobas e601 platform.

Results: Data analysis showed that 90% of the study subjects were VitD insufficient whereas incidence of folate deficiency was low since more than 85% of patients had concentrations

higher than 3 ng/ml. Serum VitD levels in patients with CRC

did not differ significantly from those of adenoma patients and

contro|s (median (lOR), 11.0 (,11.7)

vs

13.4 (14.0) vs 10.6 (12.6) ng/mL). Serum folate levels were lower in patients with

CRC

compared to those of patients with adenomas and controls (median (lOR), 4.5 (2.4) vs 5.5 (4.9) vs 6.0 (4.6)

ng/ml, respectively; P <0.05).

Conclusions: VitD deficiency was highly prevalent in patients

undergoing diagnostic colonoscopy but no differences in serum levels were observed among groups of patients. On the contrary, serum folate concentrations were significantly lower in patients with cancer but more studies are needed to relate

these results with the chemopreventive effect of the folic acid in this disease.

EuROMEDLAB 2013 - POSTERS M368

EFFECTS OF CURCUMlN SUPPLEMENTAT|ON ON SERUM FETUIN-A LEVELS lN RATS FED A HIGH-FAT D|ET lS CURCUMlN USEFUL FOR TREATMENT OF FATTY LIVER D|SEASE?

Y. Oner-l)ıidogan(ı), g. 6o""1(z), M. Seyidhanoglu(]), p. Gurdol(1), A. Gulcubuk(3), F. yildirim(3), A. cevifa), ıııı. Uys21(1)

1Depaıtment _{of Biochemistry, Facutty of Medicine, lstanbul}

University, Çapa, lstanbul, Turkey

2Depaıtment

of Biochemistry, Faculty of Medicine, tstanbut

Bilim University. Esentepe. lstanbul. Turkey

'Department of Pathology, Faculty of Veterinary Medicine,

I sta n b u l U n ive rsity. Avc ı l a r. l stan b u l. Tu rkey

qDepaı7ment

_of

_Laboratory

Animal Biology and Applied Biomedical Techniques, Institute for Experimental Research and Applied Medical Research, lstanbul University, Çapa, lstanbul, Turkey

Background: Fetuin-A (o,2 _{-Heremans-Schmid} glycoprotein)

is

mainly synthesized in the liver and secreted into the bloodstream. The clinical studies suggest the involvement of

fetuin-A in metabolic disorders such as visceral obesity, insulin

resistance,

diabetes

and fatty liver.

Curcumin

(diferuloylmethane) is extracted from tiıe rhizome Curcuma longa, and has been shown to possess potent antioxidant,

anticarcinogenic, anti-inflammatory

and

hypoglycemic

properties. ln this study, we investigated the effect of curcumin treatment on serum fetuin-A levels as well as hepatic lipids

and prooxidant-antioxidant status in rats fed high-fat diet

(HFD)

-Methods: Male Sprague-Dawley rats were divided into six groups. Group 1 was fed control diet (10ok of total calories

from fat). Group 2 and 3 were given curcumin ('100 and 400

mg/kg/b.w/day ) by gavage for B weeks, respectively. Group 4

was fed HFD (60% of total calories from fat). Group 5 and 6 received

HFD

together

with two

doses

of

curcumin.

Biochemical parameters and lipid profile were measured using

commercial

ELlSA

kits.

Prooxidant-antioxidant status parameters

were

determined

in

liver

homogenates by spectrophotometrical analysis. Protein IeveIs were determined

using bicinchoninic acid.

Results: lt was found that HFD caused triglycerides and cholesterol to accumulate in the liver but these increases were

not

associated with significant changes

in

prooxidant-antioxidant status. serum fetuin-A levels were increased in the

HFD group as compared to control and to Cur400 groups. On

the other hand, serum fetuin-A |evels were found to be

significantly reduced in both HFD+Curl00 and HFD+Cur400 groups, the reduction being 24.5% and 22.9o/o respectively. High fetuin-A levels in HFD rats did not induce any difference in insu{in resistance and dyslipidemia.

Conclusions:

All

these findings suggest that, curcumin

treatment may reduce liver fat accumulation, in correlation with fetuin-A levels. To the best of our knowledge, this is the first study demonstrating the effect of curcumin on serum fetuin-A

levels in an experimental obesity model. The reduction of fetuin-A may be considered to play an important role in the beneficial effects of curcumin treatment in obesity.