Entender os padrões de modificações pós-traducionais (PTMs) possíveis em isoformas de Mo-CBP3, empregando técnicas de biologia molecular e proteômicas (ESI-MS). Objetivos específicos
- Isolar e purificar gDNA a partir de folhas jovens de Moringa oleifera; - Amplificar a região codificadora de isoformas de Mo-CBP3;
- Clonar e sequenciar a região codificadora de isoformas Mo-CBP3;
- Identificar todas as sequências que codificam isoformas de Mo-CBP3 no genoma de M.
oleifera;
- Comparar e analisar as sequências de nucleotídeos obtidas e as sequências aminoácidos deduzidas para as isoformas de Mo-CBP3 com sequências disponíveis em bancos de dados públicos;
- Reduzir e alquilar amostras de Mo-CBP3 e submeter a análises por espectometria de massas (ESI-MS);
- Identificar todas as massas obtidas as para ambas as cadeias de Mo-CBP3;
- Calcular e associar as massas monoisotópicas (massa obtida a partir de sequência de aminoácidos) e massas calculadas (massa monoisotópica mais massa de possíveis MPTs); - Comparar, classificar todas as isoformas com base na massa calculada e a massa experimental.
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