Este trabalho permite concluir que:
O tratamento com LPS e MX não induz alterações na viabilidade celular de células monocíticas U937;
O tempo de 24 h de estimulação com LPS induziu o acúmulo de maiores concentrações de citocinas inflamatórias e foi estabelecido como modelo de inflamação celular;
O tratamento com LPS por 24 h e co-incubação com MX por mais 4 h reduziu a expressão das citocinas e quimiocinas analisadas, quando comparado ao tratamento apenas com LPS;
O bloqueio do reparo pela MX induz disfunções mitocondriais e uma cascata de respostas que podem culminar na redução da bioenergética celular e atividade da CTE mitocondrial.
As células tratadas com LPS/MX apresentam parada de ciclo celular em G1;
A APE1 é retida no núcleo, enquanto a subunidade p65 tem sua localização nuclear reduzida em células tratadas com LPS/MX, quando comparadas às células apenas estimuladas com LPS. Sob situação inflamatória, MX pode induzir estresse do RE, privação
de nutrientes e/ou acúmulo de danos ao DNA.
O tratamento LPS/MX reduz os níveis de ERO nas células, quando comparado ao tratamento com LPS;
Os níveis transcricionais de APE1 são reduzidos após o tratamento LPS/MX, contudo, não foi observada alteração nos níveis proteicos;
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