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WATER QUALITY IN AQUACULTURE

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Chlorophyll

The green pigment chlorophyll (which exists in three forms: chlorophyll a, b and c) is present in most

photosynthetic organisms and provides an indirect measure of algal biomass and an indication of the trophic status of a water body.

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Chlorophyll

It is usually included in assessment programmes for lakes and reservoirs

It is important for the management of water abstracted for drinking water supply, since excessive algal growth makes water unpalatable or more difficult to treat.

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CHLOROPHYLL

The growth of planktonic algae in a water body is

related to the presence of nutrients (principally nitrates and phosphates), temperature and light.

Therefore, concentrations of chlorophyll fluctuate seasonally and even daily, or with water depth, depending on environmental conditions.

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CHLOROPHYLL

Water bodies with low levels of nutrients (e.g.

oligotrophic lakes) have low levels of chlorophyll (< 2.5 µg l-1) whereas waters with high nutrient contents

(especially those classed as eutrophic) have high levels of chlorophyll (5-140 µg l-1), although levels in excess of 300 µg l-1 also occur.

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CHLOROPHYLL

Chlorophyll fluoresces red when excited by blue light and this property can be used to measure chlorophyll levels and

indicate algal biomass.

Direct, and continuous, measurement of chlorophyll

fluorescence can be made with a fluorimeter which can be used in situ by pumping water through it or, for some specially designed instruments, by lowering it into the water.

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CHLOROPHYLL

Samples taken for chlorophyll analysis in the laboratory should be collected in polythene bottles and 0.1 to 0.2 ml of

magnesium carbonate suspension added immediately as a preservative.

Samples should also be filtered immediately although they can be stored in a cool dark place for up to 8 hours.

The chlorophyll pigments are solvent-extracted and measured spectrophotometrically

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NUTRIENTS

NITROGEN COMPOUNDS

Nitrogen is essential for living organisms as an important constituent of proteins, including genetic material.

Plants and micro-organisms convert inorganic nitrogen to organic forms.

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NUTRIENTS

NITROGEN COMPOUNDS

In the environment, inorganic nitrogen occurs in a range of oxidation states as nitrate (NO3 ) and nitrite (NO2 ), the

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