題名: 複方五味子芝麻萃取物對於四氯化碳誘導大白鼠
Effects of schizandra and sesame extract mixture on CCl4-induced
liver injury in rats
中文摘要 本研究針對五味子（schizandra）及芝麻（sesame），探討其萃取物對四氯化碳 （CCl4）誘導肝損傷之大白鼠肝功能、抗氧化狀態及脂質代謝的影響。將六週 齡大的雄性 SD 大白鼠分成五組，每組十隻，包括 A 組:正常飲食組、B 組: CCl4 處理組、C 組: CCl4 處理組，並餵食 7.5mg/kg/day 之 silymarin、D 組: CCl4 處理 組，並餵食低劑量之複方五味子芝麻萃取物（43mg/kg/day）及 E 組: CCl4 處理 組，並餵食高劑量複方五味子芝麻萃取物（215mg/kg/day）。B、C、D 及 E 組皮 下注射 0.75mL/kg 之四氯化碳（40%四氯化碳/橄欖油, v/v），每星期一次，A 組 則以等量之橄欖油代替。實驗為期八週。結果顯示，八週後 B 組血漿中 AST 及 ALT 的活性顯著高於 A 組，而 C、D 及 E 組之 AST 及 ALT 活性與 B 組比較之 下，顯著降低。抗氧化能力方面，各組紅血球中之抗氧化酵素活性皆沒有顯著差 異。但是，B 組之肝臟中 glutathione peroxidase（GPX）的活性顯著低於 A 組， 而 D 組及 E 組之肝臟 GPX 活性顯著高於 B 組。肝臟中 glutathione reductase（GRD） 的活性在各組中皆沒有顯著差異。另外，B 組肝臟中 superoxidase dismutase（SOD） 的活性和 A 組沒有顯著差異，但 D 組及 E 組 SOD 的活性卻顯著高於 B 組。肝 臟 catalase（CAT）活性方面，B 組和 A 組沒有顯著差異，但 D 組的活性卻顯著 低於 B 組。相較於 B 組，D 組能夠顯著提高紅血球中 glutathione（GSH）濃度， 而 C、D 及 E 組皆較 B 組顯著提高肝臟中 GSH 的濃度。另外，C 組及 E 組與 B 組比較之下能夠顯著降低血漿中脂質過氧化物 malondialdehyde（MDA）濃度。 在脂質代謝方面，C、D 及 E 組在實驗末能夠顯著降低血漿及肝臟中總膽固醇濃 度，並且顯著增加血漿中高密度脂蛋白膽固醇（HDL-C）濃度。肝臟病理切片報 告則指出，B、C、D 及 E 各組雖然在肝臟纖維化方面沒有顯著差異，但是 D 組 及 E 組卻能顯著改善膽管增生及發炎反應的情形。綜合以上結果可以推測，複 方五味子芝麻萃取物在 CCl4 誘導肝臟損傷的模式下，可以藉由改善大白鼠之抗 氧化狀態以及脂質代謝的作用以達到保護肝臟的功能。 英文摘要
In this study, we examined the effects of schizandra and sesame extract mixture on carbon tetrachloride-induced liver injury in male Sprague-Dawley rats. Rats aged 6 weeks were assigned to five groups: A(control group), B(CCl4 injection), C(CCl4 injection combined with the supplementation of silymarin, 7.5mg/kg/day), D(CCl4 injection combined with the supplementation of schizandra and sesame extract mixture, 43 mg/kg/day) and E(CCl4 injection combined with the supplementation of
schizandra and sesame extract mixture, 215 mg/kg/day). Rats in group B, C, D and E were subcutaneously injected with 0.75ml/kg of 40% CCl4 dissolved in olive oil once a week, while rats in group A were injected with olive oil only. The experimental period was 8 weeks. The results indicated that rats in group C, D and E showed a significantly lower plasma AST and ALT activities than those of rats in group B (p<0.05) after eight weeks. Glutathione reductase(GRD), glutathione peroxidase (GPX), catalase(CAT) and superoxide dismutase(SOD) activities in erythrocytes showed no difference among all groups. However, hepatic SOD and GPX activities in group D and E were significantly increased than those of group B(p<0.05). Hepatic GRD activities showed no difference among all groups, and hepatic CAT activities in group D showed significantly higher than those of group B. In addition, the reduced glutathione(GSH) concentration of erythrocytes in group D showed significantly higher by 25% than that of group B(p<0.05). Compared to group B, the
MDA(malondialdehyde) concentration of erythrocytes in group C and E were significantly deceased by 42% and 25% respectively(p<0.05). The hepatic GSH concentrations in group C, D and E showed significantly increased by 48%, 45% and 53% respectively than those of group B(p<0.05). There was no difference in hepatic MDA concentrations in each group. In addition, groups C, D and E showed the significantly lower total cholesterol concentration than group B in plasma and liver. Rats in group C, D and E showed the significantly high HDL-C concentration in plasma than rats in group B. These results suggest the schizandra and sesame extract mixture showed the hepatoprotective effects by means of improving the antioxdative capacity and lipid metabolism, when rats were under CCl4-induced oxidative stress. Key words: schizandra extract、sesame extract、carbon tetrachloride、antioxdative capacity、lipid metabolism
