Costus active ingredient of anti-breast cancer
The Antitumor Constituents of Costus speciosus in Human Breast Adenocarcinoma
Abstract
Female cancer incidence of cancer in Taiwan first, how to suppress the growth of cancer cells is an important issue. In this study, 20 kinds of Taiwan to take 50%
methanol plant extracts back to two breast cancer cells (MDA-MB-231 and MCF-7 cell line) as target cells in vitro cytotoxicity test, and with the separation column, be expected to inhibit the growth of cancer cells of initial natural product, results showed Zingiberaceae Costus (Costus speciosus (Koenig) Smith)of the
Department of root growth of cancer cells were more significant, then water and ethyl acetate as Division analysis, via Diaion HP-20 column, with different proportions of water and methanol, the elution, separated by five division, of which D80Mand D100M has a strong cytotoxicity, and D100M meaningful to extend the P-388D1 fear of CDF1Cancer survival time of mice. Therefore, re-use VersaPak ® Flash, Silica gel column various separation and purification of D80M and D100M be six major components, namely (25R)-neospirost-4en-3-one (1), 25- hydroxyspirost-4-en- 3-one (2), β-D-Glucopyranoside, (3β, 25R)-17-hydroxyspirost- en-3-yl 2-Ο-(6-deoxy-α-L-mannopyranosyl) (3), Prosaponin A of dioscin (4), dioscin (5) and gracillin (6). Among them Prosaponin A ofdioscin, dioscin, gracillin human breast cancer cell cytotoxicity, IC50 were on the MDA-MB-231cells were 4.88, 3.43, 5.37 μM, on cell line MCF-7 were 4.6, 3.39,3.36 μM, and PARP
degradation makes breast cancer cells, cause apoptosis. In addition, dioscin also meaningful to extend the P-388D1of CDF1 fear cancer survival time of mice.
The above, Costus of diosgenin compounds in vivo and in vitro, all showed activity that can cause breast cancer cells, while dioscin can be used as anti-cancer activity of Costus index components or as the initial development of anticancer drugs structure, for a cancer worthy of development of cash crops.
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