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Reproduction of Bacteria

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Reproduction of Bacteria

 Reproduction in optimum media and environmental

conditions

 Reproduction is limited on laboratory conditions

 Chancing of otimum conditions (pH, osmotic pressure,

oxygen, surface tension, accumulation of toxic metabolites)

Escgerichia coli schizogenous in every 20 mins  Reaches 2114 cells in 48 hours

 Cocci are schizogeneses in the middle; basils and spirals

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Growing in liquid media

logarithmic growth

Generation

Time of generation

In E. coli 18-20 mins, in S. aureus’da 27-30 mins,

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Growing in liquid media

a) Latent period

b) Growth period (logaritmic period)

c) Standstill period

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Growing in agar medium

 Growing is restricted than liquid media

 Nutrients can’t pass to upper of colonies with diffusion  Decrease of the nutrients under the colonies

 Nutrients Located far from colony can’t reach to colony  Metabolic waste can’t thrown out of the colonies

 Degeneration of bacteria located upper of the colony because of malnutrition  Decreases of feeding areas due to contraction of the surrounding colonies  Cell degeneration in the bottom of the colony due to pressure

 Agar surface spread of toxic substances from under the colony  No free growth of bacteria in the colony

 It is hard to diffusion of nutrients in agar medium

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The factors on reproduction of bacteria

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The factors on reproduction of bacteria

 Physical Factors

Temperature (Hot - Cold)

- psychrophilic bacteria (-5 and 20 °C) - mesophilic bacteria (20 and 45 °C) - thermophilic bacteria (50 and 60 °C)

 Radiation (ionizing radiation - non-ionizing

radiation) (UV, infrared, Ultrasonic waves, X-rays, Gama rays)

 Surface tension

 Osmotic pressure (hypotonic-isotonic- hypertonic)  Hydrostatic pressure

 Damp

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The factors on reproduction of bacteria

 Biological Factors

 Resident microflora and bakteriocins (colicins)  Mechanic Factors

 Rinsing  Filtration

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