Plasma micronutrient antioxidant enzyme gene polymorphisms and arsenic metabolism and the urinary tract epithelial cancers of the research

Plasma micronutrient antioxidant enzyme gene

polymorphisms and arsenic metabolism and the urinary tract epithelial cancers of the research

Abstract

Of antioxidant enzyme manganese superoxide dismutase?

(MnSOD) and touch? (Catalase) polymorphisms and plasma micronutrients, arsenic metabolism, cigarettes and products of oxidative damage measured in urine (8-OHdG) and urinary tract risk of epithelial carcinoma of the correlation. This case-control study at National Taiwan University Hospital Department of Urology out- patient urinary tract carcinoma received 152 patients in the control group at National Taiwan University Hospital, Department of

Urology, Taipei Medical University Hospital and Taipei Municipal Wan Fang Hospital, public health examination to exclude urinary tract cancer by by age-sex matched control group received a total of 158 cases. Study after obtaining consent to a standardized

questionnaire study visits to collect demographic data, living habits, occupational exposure and personal and family medical history and collect their blood and urine samples. Using high performance liquid chromatography tandem hydride atomic absorption

spectrometry determination of urinary trivalent arsenic, pentavalent arsenic, MMA and DMA concentrations double. Extraction of blood DNA, using polymerase chain reaction (polymorphism chain

reaction; PCR) to amplify fragments. Re-use restriction fragment length polymorphisms (RFLP) analysis of manganese superoxide dismutase? And contact? Genotypes. By high performance liquid chromatography analysis of plasma levels of micronutrients. The results showed that the disease group total arsenic, inorganic arsenic and MMA significantly higher percentage, double the percentage of MMA and two methylation index significantly lower than the control group, disease group showed arsenic methylation capacity worse than the control group. The higher the

concentration of vitamin E urothelial cancer risk significantly

decreased. The lower the concentration of vitamin E and lower

secondary methylation index makes a significant increase in urinary tract carcinoma. The higher the percentage of double-MMA and the smokers had significantly increased urinary tract carcinoma. In the manganese superoxide dismutase? Genotype and touch? Genotype did not have a significant relationship with the disease. Show

arsenic metabolism is poor, the lower the concentration of vitamin E and smoking is an important urinary tract epithelial cancer risk factors. Of total arsenic, for example, if both a higher concentration of total arsenic, vitamin E concentration was low and had to smoke than those without these three risk factors, any one risk factor for urinary tract epithelial cancer odds ratio significantly increased to 16.67 (95% confidence interval 4.78-58.21).

English Abstract