Ji-fed vegetable oil or fat on the liver detoxification enzyme system and its antioxidant and anti- mutagenic effects of strength

Ji-fed vegetable oil or fat on the liver detoxification enzyme system and its antioxidant and anti-

mutagenic effects of strength

The purpose of this study, animal oil or fat oxidation in the feeding pressure, Ji-food addition on animal liver detoxification enzyme systems and the impact of antioxidant status, and to Ames test of Houttuynia cordata extract anti-mutagenicity. To dietary fat as a source of soybean oil, will be the next in the 200 ± 10 ℃ oil French fries 24 hours, as the experimental shortening (Oxidized frying oil, OFO). 5 ~ 6 weeks old Sprague-Dawley strain male rats were fed diet containing 15% fat content and different Houttuynia cordata feed the following six groups: F0, F200, F500 (fresh oil + 0, 2, 5% of the dried powder Houttuynia cordata), O0, O200, O500 (oxidized oil + 0, 2, 5% of the dried powder Houttuynia cordata), experimental period of 28 days.

The results showed that frying oil for 24 hours after the acid value, conjugated diene value, total polar material content of peroxide and were significantly higher than the fresh oil, indicating the

phenomenon of oil have been cracking and corrupt. Blood in rats fed shortening GPT, GOT and TG significantly increased the liver relative weight increased significantly. Feeding oil or fat is significantly lower plasma levels of polyphenols and polyphenol content increased stool and reduce the appearance of polyphenol absorption; Houttuynia cordata will add a significant increase in plasma levels of polyphenols; and Houttuynia cordata water extracts than the methanol extracts contain more polyphenols. Frying Oil to Cytochrome P450 (CYP) content, NADPH reductase, Ethoxyresorufin O- deethylase (EROD), Pentoxyresorufin O-depentylase (PROD), Aniline hydroxylase (ANH), Aminopyrine demethylase (AMD) and Quinone reductase (QR) activity increased significantly Among them, CYP, PROD and ANH rise the most obvious; and Houttuynia cordata will add

significantly lower EROD, ANH, AMD of the activity of the significant increase in QR activity, of which 2% of the dose control effects are obvious. Feeding oxidized oil will increase significantly in rats Thiobarbituric acid reactive substances (TBARS) concentration and protein oxidative damage occurs, and reduce the total antioxidant capacity,

and its low-density lipoprotein oxidation lag time was shorter than the fresh oil group; and Houttuynia cordata Add vegetables to extend the rats will lag time of LDL oxidation and may increase the total antioxidant capacity. Houttuynia cordata water phase and methanol extracts of the Benzo (a) pyrene, aflatoxin B1, and the

shortening of three mutagens induced suppression of both mutant power effects, and a dose relationship; and aqueous extracts

suppressed the methanol extracts better. Comprehensive results of this experiment, the oil or fat will increase oxidative stress in vivo activity of liver detoxification enzymes, and Ji-food in the animal subjected to oxidative stress of the situation, have antioxidant and anti-mutagenic effect, and have adjusted the liver the effects of detoxification enzyme activity.

English Abstract