nasopharyngeal carcinoma risk behind the loci identified in genome-wide association studies (GWAS), we conducted eQTL analyses using 25 tumor and 5 adjacent normal tis-sues. The cis- and trans-eQTLs were identified by inte-grating whole genome genotyping data with RNA-Seq gene expression data. After analysis, there were 18,375 SNP-mRNA eQTL pairs passed P < 1.00E-05 criteria. Then the eQTLs were combined with NPC GWAS loci and found HLA-B, HLA-DQ and TNFRSF19 were with putative NPC susceptibility eQTLs.
W. Su:None. J. Zhang: None. K. Chang: None.
E-P12.39
Investigation of the effect of theranekron on endoplasmic reticulum stress in human pancreatic cell line BXPC-3
V. B. Ucar, T. Duran, N. Kocak
Selcuk University, Department of Medical Genetics, Konya, TURKEY, KONYA, Turkey
Introduction: Tarantula cubensisvenom (Theranekron®) is used as a homeopathic medicine which has shown anti-tumor effects in veterinary medicine. The aim of this study was to assess effect of Tarantula cubensis on apoptotic cell death of human cancer cell lines. ER stress has also a key role in development of inflammation, obesity and cancer. Thus, ER stress has been suggested as a promising target for tumor cell death and cancer therapy. Effectiveness of ther-anekron as an anti-cancer chemical has been demonstrated in various human cancers. However, a study related to the effect of theranekron on pancreas cancer is not available in the literature. Therefore, in this study, we aimed to inves-tigate the effect of tunicamycin-induced ER stress on BXPC-3 cell line.
Materials and Methods:Effective doses of theranekron (2μg/ml) in the study were determined by MTT analysis. Cells were treated with 2μg/ml theranekron for 6 hours. Total RNA was isolated using TRIzol reagent. Synthesis of cDNA from the total RNA was carried out using Transcriptor High Fidelity cDNA Synthesis kit. Expression levels of genes (GRP78, CHOP, EDEM1, ATF4, ATF6, XBP1) associated with endoplasmic reticulum stress were analyzed by RT-qPCR. Fold changes were calculated by the ΔCT method. The statistical significances were analyzed applying two-tailed student’s t-test and analysis of variance (ANOVA).
Results: As a result, We found a difference in the expression levels of ER stress related genes in theranekron-treated BXPC-3 cells compared to the untheranekron-treated group. This suggests that theranekron effects the pathways associated with ER stress.
V.B. Ucar: None. T. Duran: None. N. Kocak: None.
E-P12.40
Can Theranekron be an agent for inducing apoptosis and ER stress in human pancreatic cells
N. Koçak1, T. Duran1, V. B. Ucar1, S. Nergiz1, I. Yildirim2
1Selçuk University, Selçuklu Medical Faculty,The Department
of Medical Genetics, Konya, Turkey,2Dicle University, Faculty of Veterinary Medicine, Department of Genetics, Diyarbakır, Turkey
Introduction: Tarantula cubensisvenom (Theranekron) is used as a homeopathic medicine. Many therapeutic effects of Theranekron, such as antitumor, necrotizing action and wound healing have been demonstrated in clinical studies. ER stress has a role in development of inflammation and cancer. Thus, ER stress has been suggested as a promising target for tumor cell death and cancer therapy. Effectiveness of theranekron as an anti-cancer chemical has been demonstrated in limited human cancer cells. However, a study related to the effect of theranekron on pancreas cancer is not available in the literature. Therefore, in this study, we aimed to investigate the effect of theranekron on ER stress and apoptosis on PANC-1.
Materials and Methods:Effective doses of theranekron (10 μl) in the study were determined by MTT analysis. Cells were treated with 10 μl theranekron for 4 hours. Total RNA was isolated using TRIzol reagent. Synthesis of cDNA from the total RNA was carried out using Transcriptor High Fidelity cDNA Synthesis kit. Expression levels of genes (GRP78, CHOP, EDEM1) associated with endoplasmic reticulum stress and genes (P53, BAX, BCL-2) associated with apoptosis were analyzed by RT-qPCR. Fold changes were calculated by the ΔCT method. The statistical significances were analyzed applying two-tailed student’s t-test and analysis of variance (ANOVA).
Results: As a result, we found a difference in the expression levels of genes associated with ER stress and apoptosis in theranekron-treated PANC-1 cells compared to the untreated group. This suggests that theranekron effects the pathways associated with ER stress and apoptosis.
N. Koçak:None. T. Duran: None. V.B. Ucar: None. S. Nergiz:None. I. Yildirim: None.
E-P12.41
Does Tarantula cubensis venom have antipoliferative & endoplasmic reticulum stress inhibitor activity on skin cancer cells?
T. Duran, N. Kocak, V. B. Ucar, T. Cora, S. Nergiz Selcuk University Medical Faculty, Konya, Turkey
